Preparation method of asiaticoside

A technology of asiaticoside and glucosinolate, which is applied in the field of preparation of asiaticoside, can solve the problem of no asiatic acid or hydroxyasiatic acid, etc., and achieve high product yield and purity, and few by-products , the effect of little pollution

Pending Publication Date: 2020-08-11
LUNAN PHARMA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the preparation of asiatic acid or hydroxyasiatic acid by enzymatic hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0028] (1) Enzymolysis reaction Add 10 g of asiaticoside into a buffer solution composed of water, acetic acid and sodium acetate, so that the final concentration of the substrate is 10 g / L, and the pH of the buffer solution is 5.0. Add helicase to the reaction system at a mass ratio of substrate to enzyme of 1:4, and react at 45°C for 84 hours;

[0029] (2) Suction filtration Suction filtration of the reaction solution in step (1), discarding the filtrate, dissolving the filter cake with dehydrated ethanol, the weight-to-volume ratio of asiaticoside and dehydrated alcohol is 1:25, and the solution is extracted Filtration, the filtrate is retained, the resulting filter cake repeats the above operation once, and merges the filtrate twice;

[0030] (3) Crystallization Concentrate the filtrate obtained in step (2) to saturation, add water to crystallize until the alcohol content is below 20%, carry out suction filtration to the crystallization liquid, discard the filtrate, dry th...

specific Embodiment 2

[0032] (1) Enzymolysis reaction Add 10 g of asiaticoside into a buffer solution composed of water, acetic acid and sodium acetate, so that the final concentration of the substrate is 10 g / L, and the pH of the buffer solution is 5.0. Add naringinase to the reaction system at a mass ratio of substrate to enzyme of 1:4, and react at 45°C for 84 hours;

[0033] (2) Suction filtration Suction filtration of the reaction solution in step (1), discarding the filtrate, dissolving the filter cake with dehydrated ethanol, the weight-to-volume ratio of asiaticoside and dehydrated alcohol is 1:25, and the solution is extracted Filtration, the filtrate is retained, the resulting filter cake repeats the above operation once, and merges the filtrate twice;

[0034] (3) Crystallization Concentrate the filtrate obtained in step (2) to saturation, add water to crystallize until the alcohol content is below 10%, carry out suction filtration to the crystallization liquid, discard the filtrate, dry...

specific Embodiment 3

[0036] (1) Enzymolysis reaction Add 10 g of asiaticoside into a buffer solution composed of water, acetic acid and sodium acetate, so that the final concentration of the substrate is 10 g / L, and the pH of the buffer solution is 5.0. Add cellulase to the reaction system at a mass ratio of substrate to enzyme of 1:4, and react at 45°C for 84 hours;

[0037] (2) suction filtration The reaction solution in step (1) is suction filtered, the filtrate is discarded, and the filter cake is dissolved in absolute ethanol. The weight-to-volume ratio of asiaticoside and absolute alcohol is: 1:25. Suction filtration, the filtrate is retained, the obtained filter cake repeats the above operation once, and the filtrate is combined twice;

[0038] (3) Crystallization Concentrate the filtrate obtained in step (2) to saturation, add water to crystallize until the alcohol content is below 30%, carry out suction filtration to the crystallization liquid, discard the filtrate, dry the filter cake at...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method of asiaticoside. An enzymolysis method of asiaticoside provided by the invention has obviously higher yield and purity than those in an acid-base hydrolysis method, has low requirements on equipment, has the advantages of small environmental pollution, no environmental pressure and the like, and provides an effective method for researching and developing active substances of traditional Chinese medicine centella asiatica.

Description

Background technique [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of asiaticoside. Background technique [0002] Centella asiatica (Centella asiatica) is a plant of the genus Centella asiatica. As a traditional medicinal plant, it has a long history and is widely used in my country. Its components are mostly triterpenoids, of which Asiaticoside (AT) Because of its rich pharmacological activity and wide range of clinical effects, it has become the main active ingredient in Centella asiatica, with great application prospects and great development value. In recent years, a large number of studies have confirmed that AT has various pharmacological activities such as anti-tumor, anti-venous insufficiency, anti-ulcer, promoting wound healing, anti-depression, and recovery of nerve function. Pharmacological effects of viruses, etc. [0003] Madecassoside, madecassoside, asiaticoside B, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00
CPCC12P33/00
Inventor 张贵民陈小荣韩廷伟
Owner LUNAN PHARMA GROUP CORPORATION
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