Preparation method of asiaticoside
A technology of asiaticoside and glucosinolate, which is applied in the field of preparation of asiaticoside, can solve the problem of no asiatic acid or hydroxyasiatic acid, etc., and achieve high product yield and purity, and few by-products , the effect of little pollution
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specific Embodiment 1
[0028] (1) Enzymolysis reaction Add 10 g of asiaticoside into a buffer solution composed of water, acetic acid and sodium acetate, so that the final concentration of the substrate is 10 g / L, and the pH of the buffer solution is 5.0. Add helicase to the reaction system at a mass ratio of substrate to enzyme of 1:4, and react at 45°C for 84 hours;
[0029] (2) Suction filtration Suction filtration of the reaction solution in step (1), discarding the filtrate, dissolving the filter cake with dehydrated ethanol, the weight-to-volume ratio of asiaticoside and dehydrated alcohol is 1:25, and the solution is extracted Filtration, the filtrate is retained, the resulting filter cake repeats the above operation once, and merges the filtrate twice;
[0030] (3) Crystallization Concentrate the filtrate obtained in step (2) to saturation, add water to crystallize until the alcohol content is below 20%, carry out suction filtration to the crystallization liquid, discard the filtrate, dry th...
specific Embodiment 2
[0032] (1) Enzymolysis reaction Add 10 g of asiaticoside into a buffer solution composed of water, acetic acid and sodium acetate, so that the final concentration of the substrate is 10 g / L, and the pH of the buffer solution is 5.0. Add naringinase to the reaction system at a mass ratio of substrate to enzyme of 1:4, and react at 45°C for 84 hours;
[0033] (2) Suction filtration Suction filtration of the reaction solution in step (1), discarding the filtrate, dissolving the filter cake with dehydrated ethanol, the weight-to-volume ratio of asiaticoside and dehydrated alcohol is 1:25, and the solution is extracted Filtration, the filtrate is retained, the resulting filter cake repeats the above operation once, and merges the filtrate twice;
[0034] (3) Crystallization Concentrate the filtrate obtained in step (2) to saturation, add water to crystallize until the alcohol content is below 10%, carry out suction filtration to the crystallization liquid, discard the filtrate, dry...
specific Embodiment 3
[0036] (1) Enzymolysis reaction Add 10 g of asiaticoside into a buffer solution composed of water, acetic acid and sodium acetate, so that the final concentration of the substrate is 10 g / L, and the pH of the buffer solution is 5.0. Add cellulase to the reaction system at a mass ratio of substrate to enzyme of 1:4, and react at 45°C for 84 hours;
[0037] (2) suction filtration The reaction solution in step (1) is suction filtered, the filtrate is discarded, and the filter cake is dissolved in absolute ethanol. The weight-to-volume ratio of asiaticoside and absolute alcohol is: 1:25. Suction filtration, the filtrate is retained, the obtained filter cake repeats the above operation once, and the filtrate is combined twice;
[0038] (3) Crystallization Concentrate the filtrate obtained in step (2) to saturation, add water to crystallize until the alcohol content is below 30%, carry out suction filtration to the crystallization liquid, discard the filtrate, dry the filter cake at...
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