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A new process of extracting alginate oligosaccharides from kelp by enzymatic hydrolysis

A technology of alginate oligosaccharide and enzymatic hydrolysis method is applied in the field of extraction technology of alginate oligosaccharide, which can solve problems such as reducing the use of chemical reagents, and achieve the effects of improving product yield, mild reaction conditions and ensuring food safety.

Active Publication Date: 2015-12-09
青岛聚大洋藻业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the existing problems in the extraction of algin oligosaccharides, and provides a production process for extracting algin oligosaccharides from kelp. The process steps are simplified, enzymatic hydrolysis is adopted, the use of chemical reagents is reduced, and the appearance of the product is more beautiful

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  • A new process of extracting alginate oligosaccharides from kelp by enzymatic hydrolysis

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Experimental program
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Effect test

Embodiment 1

[0025] (1) Prepare sea-tangle powder: cut the washed sea-tangle into pieces, pulverize and sieve to obtain sea-tangle powder, re-grind and sieve the unsifted sea-tangle, and use a 100-mesh sieve for sieving;

[0026] (2) Soaking: put the kelp powder into the enzymolysis tank, add water 8 times the weight of the kelp powder, and stir until fully soaked;

[0027] (3) Cellulase enzymolysis: put 1600U / g cellulase preparation into the enzymolysis tank, the addition is 8% of the weight of kelp powder, stir well, add 10% hydrochloric acid solution to adjust the pH to 4-5 , enzymatic hydrolysis at a temperature of 55° C. for 3 hours to obtain a cellulase hydrolysis product;

[0028] (4) Digestion: Add 8% sodium carbonate by weight of kelp powder and hot water 10 times the weight of kelp powder to the cellulase hydrolyzate, and digest for 2 hours at a temperature of 50°C to obtain sodium alginate gel thing;

[0029] (5) Centrifugal filtration: the obtained sodium alginate jelly is se...

Embodiment 2

[0036] (1) Prepare sea-tangle powder: cut the cleaned sea-tangle into pieces, pulverize and sieve to obtain sea-tangle powder, re-grind and sieve the unsifted sea-tangle; use a 60-mesh sieve for sieving;

[0037] (2) Soaking: put the kelp powder into the enzymolysis tank, add water 12 times the weight of the kelp powder, and stir until fully soaked;

[0038] (3) Cellulase enzymolysis: put 1200U / g cellulase preparation into the enzymolysis tank, the addition amount is 12% of the weight of kelp powder, stir evenly, add 10% hydrochloric acid solution to adjust the pH to 4-5 , enzymatic hydrolysis at a temperature of 65° C. for 4 hours to obtain a cellulase hydrolysis product;

[0039] (4) Digestion: Add 13% sodium carbonate of kelp powder weight and 15 times hot water of kelp powder weight to the cellulase hydrolyzate, and digest for 3 hours at a temperature of 80° C. to obtain sodium alginate gel thing;

[0040] (5) Centrifugal filtration: the obtained sodium alginate jelly is...

Embodiment 3

[0047] (1) Prepare sea-tangle powder: cut the cleaned sea-tangle into pieces, pulverize and sieve to obtain sea-tangle powder, and re-grind and sieve the unsifted sea-tangle; sieve with a 90-mesh sieve;

[0048] (2) Soaking: Put the kelp powder into the enzymolysis tank, add water 10 times the weight of the kelp powder, and stir until fully soaked;

[0049] (3) Cellulase enzymolysis: put 1400U / g cellulase preparation into the enzymolysis tank, the addition is 10% of the weight of kelp powder, stir well, add 10% hydrochloric acid solution to adjust the pH to 4-5 , enzymolysis at 60°C for 3.5 hours to obtain a cellulase hydrolysis product;

[0050] (4) Digestion: Add 11% sodium carbonate of kelp powder weight and 12 times hot water of kelp powder weight to the cellulase enzymolysis product, and digest for 150 minutes at a temperature of 60°C to obtain sodium alginate gel thing;

[0051] (5) Centrifugal filtration: the obtained sodium alginate jelly is separated from the residu...

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Abstract

By aiming at the problems in the existing alginate-derived oligosaccharide extraction, the invention provides a production process for extracting alginate-derived oligosaccharide from sea tangles. A novel process for extracting alginate-derived oligosaccharide from sea tangles by an enzyme hydrolysis method comprises the nine steps of sea tangle powder preparation, soaking, cellulose enzymolysis, digestion, centrifugal filtration, bleaching, sodium alginate lyase enzymolysis, high-speed centrifugal fine filtration and alcohol dehydration. Compared with the existing alginate-derived oligosaccharide extraction process with the yield being 14-15 percent, the novel process has the advantages that the yield is 17-18 percent and is improved by 3 to 4 percent, meanwhile, the process steps are simplified, the use of chemical reagents is reduced, and the product appearance is more attractive.

Description

technical field [0001] The invention relates to a process for extracting algin oligosaccharides, in particular to a production process for extracting algin oligosaccharides from kelp. Background technique [0002] Alginate is the collective name of alginic acid, alginate and organic derivatives of alginic acid. Since its inception, alginate has been widely used in printing and spinning, food, medicine, chemical industry and agriculture. Domestic manufacturers mostly use dried kelp as raw material, use traditional techniques to extract alginate, and prepare alginate oligosaccharides. The traditional process generally includes steps such as soaking, digesting, diluting, centrifuging to remove slag, calcium precipitation, decalcification, solid-phase conversion and drying. This traditional process has the following deficiencies: (1) the block kelp is digested with lye, The lye cannot completely destroy the cell wall, resulting in a relatively low extraction rate of alginate in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/00
Inventor 程跃谟吴仕鹏郭华玉于云霞孙梅殷聪
Owner 青岛聚大洋藻业集团有限公司
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