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A kind of method for efficiently extracting full-component centipede algae extract by double-enzyme method

A technology of centipede algae and full ingredients, which is applied in the field of efficient extraction of full-component centipede algae extract, can solve the problems of difficult control of addition conditions, loss of active ingredients, cumbersome extraction methods, etc., to save labor and material resources, reduce addition amount, and enhance immunity force effect

Inactive Publication Date: 2016-03-30
GUANGDONG SHAOHE PEARL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, existing methods are difficult to extract components such as cysteine, histidine, proline, tryptophan, zinc, iron, etc., resulting in the loss of extracted active ingredients.
In addition, in the prior art, hydrochloric acid and sulfuric acid are often used to adjust the pH. Hydrochloric acid and sulfuric acid are highly acidic, highly corrosive, difficult to control the addition conditions, difficult to operate during the implementation process, and the extraction method is cumbersome, and the safety of the extracts for food and cosmetics is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Weigh 2 kg of Centipede Algae, add 70 kg of deionized water, stir well, add 7.2 g of anhydrous sodium carbonate, and adjust the pH to 7. Add 200g of agarase, stir well, and heat in the reaction pot at 25°C for 8h. Add 144g of potassium sorbate and 23mL of lactic acid, stir well, and adjust the pH to 5.0. Add 122g of cellulase, stir well, and heat in the reaction pot at 50°C for 12h. After the reaction, the enzymolysis solution was centrifuged (the residue was 576g). Add 22g of anhydrous sodium carbonate to the centrifuged supernatant, adjust the pH to 7, heat to 100°C to sterilize and eliminate enzymes, and the obtained solution is the extract of the whole centipede algae, which is left to cool for later use. The extraction rate of the whole composition centipede algae extract is 27.74%, and the yield rate is 92.47%.

Embodiment 2

[0034] Example 2: Weigh 2 kg of Centipede Algae, add 70 kg of deionized water, stir well, add 8.4 g of anhydrous sodium carbonate, and adjust the pH to 7.5. Add 240g of agarase, stir well, and heat in the reaction pot at 25°C for 8h. Add 144g of potassium sorbate and 25mL of lactic acid, stir well, and adjust the pH to 5.1. Add 140g of cellulase, stir well, and heat in the reaction pot at 52°C for 12h. After the reaction, the enzymolysis solution was centrifuged (the residue was 920g). Add 20 g of anhydrous sodium carbonate to the centrifuged supernatant, adjust the pH to 7, heat to 100° C. to sterilize and eliminate enzymes, and the obtained solution is the whole-component centipede algae extract, which is left to cool for later use. The extraction rate of the whole composition centipede algae extract is 26.83%, and the yield rate is 89.43%.

Embodiment 3

[0035] Example 3: Weigh 2 kg of Centipede algae, add 70 kg of deionized water, stir well, add 7.2 g of anhydrous sodium carbonate, and adjust the pH to 7. Add 144g of agarase, stir well, and heat in the reaction pot at 25°C for 8h. Add 144mL potassium sorbate and 23mL lactic acid, stir well, and adjust the pH to 5.0. Add 72g of cellulase, stir well, and heat in the reaction pot at 50°C for 12h. After the reaction, the enzymolysis solution was centrifuged (the residue was 1728g). Add 22g of anhydrous sodium carbonate to the centrifuged supernatant, adjust the pH to 7, heat to 100°C to sterilize and eliminate enzymes, and the obtained solution is the extract of the whole centipede algae, which is left to cool for later use. The extraction rate of the whole composition centipede algae extract was 24.53%, and the yield rate was 81.77%.

[0036] In this example, due to the reduction in the addition of agarase and cellulase, the cell wall was not completely destroyed, the insolub...

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Abstract

The method for efficiently extracting the full-component centipede algae extract by the double-enzyme method of the present invention comprises the following steps: adding water and stirring the centipede algae to form a base material; adding anhydrous sodium carbonate to adjust the pH to 6.5-7.5; and then weighing 0.25%- Agarase with 0.39% base material weight is dissolved in water and added to the above base material with adjusted pH value for enzymolysis. The enzymolysis temperature is 20-35°C and the enzymolysis time is 7-9 hours; add lactic acid, Potassium sorbate, stir evenly, and control the pH at 4.8-5.2; then weigh 0.17%-0.22% of the base material weight cellulase, dissolve it in water, add it to the previous solution for enzymolysis, and the enzymatic hydrolysis temperature is 45-55°C , the enzymolysis time is 11-14 hours; after the reaction, centrifuge or filter to obtain the clear liquid; add anhydrous sodium carbonate to the clear liquid to adjust the pH to 6.5-7.5, then heat to sterilize the enzyme, and the obtained solution is Full ingredients centipede algae extract. The invention ensures the purity of the whole component centipede algae extract and effectively improves the extraction rate of the effective components of centipede algae.

Description

technical field [0001] The invention relates to a method for extracting seaweed, in particular to a method for efficiently extracting the whole component centipede algae extract by a double-enzyme method. Background technique [0002] At present, my country is the main seaweed producing country in the world, harvesting more than 1.5 million tons of various seaweeds (according to dry products) every year. These seaweeds are used to produce alginates and esters, agar, carrageenan, and are widely used in processed food, food additives, pharmaceuticals, chemical products and other industries. Studies have shown that the active substances of seaweed include seaweed polysaccharides, seaweed oligosaccharides, small molecular peptides and various vitamins and trace elements, which can improve blood circulation in the brain, enhance myocardial function, and prevent cardiovascular diseases. It has special effects on cerebral thrombosis, etc., and at the same time can enhance human im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/04
Inventor 庄铠燕林展新谢绍河
Owner GUANGDONG SHAOHE PEARL
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