Preparation method of enzymatic soybean meal and application of enzymatic soybean meal in largemouth black bass feed
An enzymatic hydrolysis of soybean meal, enzymatic hydrolysis technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of anti-nutritional factors and poor palatability, and achieve the effect of improving feed value, improving feed utilization rate, and improving immune function.
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[0024] The invention provides a method for preparing enzymatically hydrolyzed soybean meal and the application of the enzymatically hydrolyzed soybean meal in largemouth bass feed.
[0025]
[0026] Preparation of enzymatically hydrolyzed soybean meal: the peeled soybean meal is firstly hydrolyzed by enzyme preparations such as non-starch polysaccharase and phytase, and then enzymatically hydrolyzed by neutral protease. specifically,
[0027] The preparation method of enzymolysis soybean meal of the present invention comprises the following steps:
[0028] (1), the enzyme preparation is dissolved in acetic acid-sodium acetate buffer and mixed uniformly to obtain a mixture, which is added to the dehulled soybean meal so that the content of the dehulled soybean meal is 50wt% and reacted at 45-55°C for 40- After 50 minutes, the reaction is completed and the temperature is raised to 90-100°C to inactivate the enzyme to terminate the enzymatic hydrolysis reaction;
[0029] (2),...
Embodiment 1
[0042] Preparation of enzymatic soybean meal:
[0043] (1), dissolving phytase, pectinase, cellulase, xylanase and α-galactosidase in a 0.2mol / L acetic acid-sodium acetate buffer solution with a pH of 5.0 and mixing uniformly to obtain a mixture , the addition amounts of phytase, pectinase, cellulase, xylanase and α-galactosidase were 10.5U / g, 16.0U / g, 50.0U / g, 80.0U / g and 0.7 U / g, weigh a certain mass of dehulled soybean meal, add the mixture to the dehulled soybean meal, make the content of the dehulled soybean meal 50wt%, and react at 50°C for 45min, immediately raise the temperature to 90°C to inactivate the enzyme to terminate the enzymatic reaction.
[0044] (2) Continue adding distilled water to the reaction system in step (1), so that the content of dehulled soybean meal is 15 wt%, and adjust the pH value of the reaction system to 7.5.
[0045] (3) Add neutral protease to the reaction system of step (2) and enzymatically hydrolyze it at 50°C for 6 hours. The amount o...
Embodiment 2
[0049]Application of enzymatically hydrolyzed soybean meal in compound feed for largemouth bass. In the largemouth bass feed, the protein was added to the dehulled soybean meal (15wt%), the commercial soybean meal fermented with complex strains (lactic acid bacteria, bacillus and yeast complex fermentation), the commercial yeast fermented soybean meal and the above-mentioned self-made enzymatic soybean meal, Configure four kinds of feed such as protein and fat. The feed formulation is shown in Table 2. All low-fat raw materials are evenly mixed according to the feed formula after being crushed and passed through an 80-mesh sieve. Thereafter, the mixed feed fat source (ie basal feed) was uniformly mixed with the above mixture and passed through a 40-mesh sieve. Afterwards, add water and knead evenly and pass through a 40-mesh sieve, and granulate with a 2.5mm die hole of a granulator. Subsequently, the above-mentioned feed was gelatinized in an oven at 105° C. for 15 minutes...
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