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Aerobic denitrifying pseudomonas and culture method and application thereof

A technology of aerobic denitrification and pseudomonas, applied in the field of microorganisms, can solve problems such as limitations, and achieve the effect of strong tolerance and degradation ability

Active Publication Date: 2020-08-14
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This limits the application of such strains to treat wastewater containing high concentrations of organic matter and nitrogen pollution

Method used

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  • Aerobic denitrifying pseudomonas and culture method and application thereof
  • Aerobic denitrifying pseudomonas and culture method and application thereof
  • Aerobic denitrifying pseudomonas and culture method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] The present invention will be further described below in conjunction with specific preferred embodiment, but does not limit protection scope of the present invention thereby.

[0050] Sampling source: Activated sludge from pig farm wastewater treatment in Longjin Xiudong, Longyan City, Fujian Province.

[0051] Table 1 Medium name and formula

[0052]

[0053] Note: Before use, the medium needs to be sterilized at 121°C for 20 minutes.

[0054] Table 2 Experimental Instruments

[0055]

[0056]

[0057] Table 3 Water quality testing indicators and analysis methods

[0058] index Analytical method NH 4 + -N

Nessler's reagent spectrophotometry [16]

NO 3 - -N

Thymol Spectrophotometry [17]

NO 2 - -N

Ethylenediamine Spectrophotometry [18]

TN Alkaline potassium persulfate digestion spectrophotometry [19]

COD COD instrument digestion method [20]

pH UB-7 Precision pH Meter ...

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Abstract

The invention discloses an aerobic denitrifying pseudomonas which is pseudomonas XD9 (Pseudomonas alcaliphila XD9) and is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) for thepreservation time of December 25, 2018, and the preservation number is GDMCCNo:60534. The pseudomonas has strong heterotrophic nitrification and aerobic denitrification characteristics, and also hasthe capability of tolerating high-concentration organic matters, NH4<+>-N, NO3<->-N and NO2<->-N. Therefore, the pseudomonas has obvious application advantages in the aspect of treating NH4<+>-N wastewater with high organic matter content, and has application potential in the aspect of treating actual high-concentration NO3<->-N and NO2<->-N wastewater.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an aerobic denitrifying pseudomonas and a culture method and application thereof. Background technique [0002] In recent years, with the rapid development of science and technology and the continuous progress of society, human needs are constantly increasing. Large-scale production in industries such as mariculture, industry and agriculture, seafood processing, and petroleum production has led to a large amount of inorganic nitrogen (mainly ammonia nitrogen, nitric acid, etc.) Salt nitrogen, nitrite nitrogen) discharged into the water body, not only causes eutrophication of the water body, pollutes the water ecological environment, seriously affects the water quality, but also causes aquatic animals to lose their balance, anorexia, slow movement, and even death due to excessive nitrogen content . [0003] Traditional biological denitrification includes aerobic nitrificat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC12N1/20C02F3/34C02F2101/16C02F2101/163C02F2101/166C12R2001/38C12N1/205Y02W10/10
Inventor 孙巍夏春雨阙才英苏全榕韦明肯魏登枭林彩琴
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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