Method for simultaneously detecting acetamiprid and malathion
A technology for malathion and acetamiprid, which is applied in the field of simultaneous detection of acetamiprid and malathion, can solve the problems of time-consuming and high detection costs, and achieves improved stability, efficient immobilization, and good binding ability. Effect
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Embodiment 1
[0030] Example 1 Preparation of hollow copper-cobalt-MOF:
[0031] (1) Take 1.83 g Co(NO 3 ) 2 The solid was mixed with 20 mL methanol solution (0.5 mol / L), and 1.88 g Cu(NO 3 ) 2 Solid with 20 mL of methanol solution (0.5 mol / L), take 1.3456 g of tetradecyltrimethylammonium bromide solid with 40 mL of aqueous solution (0.1 mol / L), take 1.7995 g of dimethylimidazole solid with 20 mL Methanol solution (1.096 mol / L);
[0032] (2) Add 375 μL 0.5 mol / L Co(NO 3 ) 2 methanol solution, 250 μL 0.5 mol / L Cu(NO 3 ) 2 Methanol solution and 10 mL of 0.1 mol / L tetradecyltrimethylammonium bromide aqueous solution; 63.75 mL of deionized water was added, and the mixture was magnetically stirred for 5 minutes, followed by the immediate addition of 6.5 mL of 1.096 mol / L dimethylimidazole Methanol solution; the reaction solution was continuously stirred for 90 minutes, and then the product was collected, washed with N-N-dimethylformamide and centrifuged twice, then washed with methanol a...
Embodiment 2
[0033] Example 2 g-C composited with gold nanoparticles 3 N 4 Preparation of:
[0034] (1) Weigh 10 g of urea into a crucible with an electronic balance, then put it into a tube furnace, heat up to 550 °C at a rate of 5 °C / min, and keep it warm for 4 h to obtain a light yellow powder C 3 N 4 ; Weigh the obtained C 3 N 4 100 mg in a conical flask, add 100 mL of ultrapure water, then sonicate for 16 h, the resulting solution is centrifuged at 3000 r / s to remove undispersed C 3 N 4 , collect the supernatant, and centrifuge at 10000r / s to obtain a precipitate, which is dissolved in 5 mL of ethanol. get a C 3 N 4 weak;
[0035] (2) Use a pipette to pipette 5 mL of C 3 N 4 Add 60 mL of AuNPs solution to the solution and test tube, put it on a magnetic stirrer and stir for 12 hours, centrifuge at 8500 r / s for 5 minutes in a centrifuge, and dissolve the obtained precipitate in 5 mL of ethanol solution to obtain g-C 3 N 4 -AuNPs solution.
Embodiment 3
[0036] Example 3 Preparation of complementary chain of acetamiprid aptamer loaded on hollow copper-cobalt-MOF:
[0037] (1) Take 1 mL of AuNPs in a beaker, add 20 mg of hydroxysuccinimide and 10 mg of carbodiimide, stir well, put it in an oven and incubate at 37 °C for 2 h, then add 1 mL of 0.01 mol / L The luminol solution was placed in an oven and incubated at 37 °C for 1 h to prepare the Luminol-AuNPs solution;
[0038] (2) Add 1 mL of Luminol-AuNPs to 1 mL of hollow copper-cobalt-MOF and stir on a magnetic stirrer for 12 h to prepare hollow copper-cobalt-MOF-Luminol-AuNPs;
[0039] (3) Use a pipette gun to pipette 20 μL of 0.1 μmol / L acetamiprid aptamer chain and slowly add it to 200 μL of hollow copper-cobalt-MOF-Luminol-AuNPs solution, shake it on the shaker for 30 minutes to shake homogeneously, and then placed in the refrigerator and incubated at 4 °C for 8 h to prepare hollow copper-cobalt-MOF-Luminol-AuNPs-cDNA1.
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