Preparation special for root irrigation application and capable of increasing rice chlorophyll a content
A technology for chlorophyll and rice is applied in the field of preparations for increasing the content of chlorophyll a in plants, and can solve the problems of unpredictable results, unpredictable effects and the like, and achieve the effects of convenient agricultural production, significant effects and stable effects.
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Embodiment 1
[0031] Embodiment 1, experimental design
[0032] Screening plant growth regulating substances for root application and effectively increasing the content of plant chlorophyll a has been the research direction of the inventors for many years. During this period, the inventors conducted root application experiments on six kinds of plants including rice, and screened more than 20 plant growth regulating substances and their combinations. It was found that the combination of S-absorbin and fulvic acid showed stable and good effects in many experiments, especially, the compound preparation of S-abactin and fulvic acid was used in the experiment of increasing the content of chlorophyll a in rice. showed a very significant advantage. Therefore, in order to highlight the superiority of the compound preparation of the present invention, this example will specifically demonstrate: the effect of applying common nutrients, the effect of applying S-abenatin or fulvic acid alone, the effe...
Embodiment 2
[0042] Embodiment 2, chlorophyll a measuring method and synergistic effect
[0043] Three pieces of leaves at the stage of milk maturity were taken from the experimental rice from top to bottom, and cut into pieces with scissors (less than 1 cm in length), and the pieces of each leaf were weighed, each with a fresh weight of 2 g, put into a stoppered Erlenmeyer flask, and mixed with 95 % ethanol is used as the extraction solution, the extraction time is 8h, and the triangular flask is placed in the dark, the extraction solution is carefully poured into a 100ml volumetric flask, and the volume is adjusted to 100ml. After shaking well, draw 5ml of this solution into a 50ml volumetric flask, dilute to 50ml with 95% ethanol, then measure the absorbance at 649nm and 665nm respectively with a spectrophotometer and calculate the concentration of chlorophyll a, and then convert it into fresh weight Chlorophyll a content (mg / gFW).
[0044] The calculation formula is: Ca=13.95D 665 -6...
Embodiment 3
[0049] Embodiment 3, the measurement of other index of plant
[0050] Because the content of chlorophyll a is beneficial to the photosynthesis of plants, it can produce more nutrients and promote plant development. Therefore, in order to corroborate the above-mentioned results, the present invention has further measured the indexes such as plant height, leaf height, leaf width, rate of ear formation and morphology of plants after adopting common nutrient and the compound preparation of the present invention.
[0051] The measurement method of plant height is: measure from the rhizome to the top growth point with a ruler.
[0052] The method of measuring the leaf height is: measure with a ruler from the root of the leaf to the top of the leaf.
[0053] The method of measuring leaf width is: take the middle position of the leaf, and measure the positions at both ends with a ruler.
[0054] The measuring method of earing rate is: counting the number of plants with earing in the...
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