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Applications of natural host defense peptide CATHPb1

A host defense peptide, a natural technology, applied in the field of peptide applications, can solve the problems of bacterial drug resistance drug residues, unscientific and other problems, and achieve the effects of broad-spectrum and high-efficiency antibacterial effects, improving resistance, and wide application prospects.

Pending Publication Date: 2020-08-25
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, with the extensive use of antibiotics in the aquaculture industry, especially the unscientific abuse, problems such as bacterial resistance and drug residues have become increasingly prominent

Method used

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  • Applications of natural host defense peptide CATHPb1
  • Applications of natural host defense peptide CATHPb1
  • Applications of natural host defense peptide CATHPb1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of CATHPb1

[0026] (1) Using an automatic peptide synthesizer (433A, Applied Biosystems) to synthesize the complete sequence of CATHPb1 derived from the reptile Burmese python, and desalting and purifying it by HPLC reverse-phase column chromatography.

[0027] (2) The molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF).

[0028] (3), the purity of the purified CATHPb1 is identified by high performance liquid chromatography (HPLC), the molecular weight is determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF), the isoelectric point is determined by isoelectric focusing electrophoresis, and it is determined by an automatic amino acid sequencer Amino acid sequence structure.

[0029] The amino acid sequence of the natural host defense peptide CATHPb1 of the present invention is shown in SEQ ID No.1. It consists of 31 amino acids,...

Embodiment 2

[0030] Example 2 Detection of antibacterial activity of CATHPb1 on common aquatic pathogens

[0031] (1), respectively pick the test strains (aquatic pathogenic bacteria) preserved on the slant and smear them evenly on the nutrient broth solid medium (nutrient broth medium, British OXOID company) flat plate, the 0.5cm diameter through sterilization Place the filter paper on the surface of the culture medium, drop 10 μL of 2 mg / mL CATHPb1 sample solution dissolved in sterilized deionized water, incubate upside down at 37°C for 18-20 hours, and observe whether the inhibition zone is formed or not. If the sample has antibacterial activity, a clear and transparent bacteriostatic zone will be formed around the filter paper, and the larger the bacteriostatic zone, the stronger the antibacterial activity of the sample.

[0032] (2), CATHPb1 minimum inhibitory concentration (Minimum Inhibitory Concentration) determination (2-fold dilution method):

[0033] Select the strains with the...

Embodiment 3

[0042] Example 3 Determination of CATHPb1 sterilization speed

[0043] Vibrio parahaemolyticus was cultured at 37°C for 12 hours with NB liquid medium (OXOID, UK), and then diluted to 10 with fresh NB liquid medium. 6 CFU / mL bacterial suspension. The CATHPb1 sample dissolved in sterilized deionized water was added to the bacterial suspension so that the final concentration was 5×MIC. Place the bacterial solution added with the CATHPb1 sample in a 37°C incubator for shaking culture, take 50 μL of the bacterial solution to dilute 1000 times at 0, 15, 30, 60, 120 and 180 minutes respectively, and then take 50 μL of the diluted bacterial solution and spread it on the NB Count the colonies on the solid medium plate after culturing overnight in a 37°C incubator. In this experiment, neomycin sulfate was used as a positive control, and sterilized deionized water was used as a negative control.

[0044] The results are shown in Table 3, CATHPb1 has a rapid bactericidal speed to Vibr...

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Abstract

The present invention relates to applications of a natural host defense peptide CATHPb1, particularly relates to the application of the natural host defense peptide CATHPb1 in preparation of medicinesagainst aquatic pathogenic bacteria and the application in preparation of aquaculture animal immunomodulators, and further discloses the application of the natural host defense peptide CATHPb1 in preparation of aquaculture animal feeds. Micropterus salmoides is used as experimental animals and study results show that the CATHPb1 can kill a variety of common aquatic pathogenic bacteria, regulatesimmune functions of the micropterus salmoides, improves resistance of the micropterus salmoides to bacterial infection, improves a survival rate of the micropterus salmoides, and has broad applicationprospects in the field of aquaculture.

Description

technical field [0001] The invention relates to the field of polypeptide applications, in particular to the application of a natural host defense peptide CATHPb1. Background technique [0002] Antibiotics are chemicals produced by microorganisms that, at low concentrations, inhibit or kill other microorganisms. Antibiotics have played an important role in the development of aquaculture because of their functions of promoting animal growth, improving feed utilization, and preventing and treating animal diseases. [0003] However, with the extensive use of antibiotics in the aquaculture industry, especially the unscientific abuse, problems such as bacterial resistance and drug residues have become increasingly prominent. Due to problems such as excessive antibiotic residues, the export volume of my country's aquaculture products only accounts for 0.9%-1.2% of production, which seriously affects the development of my country's aquaculture economy. Therefore, it is increasingl...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P31/04A61P37/02A23K50/80A23K20/147
CPCA61K38/1703A61P31/04A61P37/02A23K50/80A23K20/147
Inventor 王义鹏于海宁杨怀欣章铭辉欧阳建红
Owner SUZHOU UNIV
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