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Antibacterial peptide MS-CATH from Miniopterus fuliginosus and application thereof

A technology of MS-CATH and antimicrobial peptides, applied in the field of biomedicine, can solve problems such as cell death, damage to cell membrane integrity, dissolution of cell contents, etc., and achieve low hemolytic activity, broad-spectrum high-efficiency antibacterial effect, and rapid bactericidal effect

Active Publication Date: 2021-10-08
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bactericidal mechanism of most antimicrobial peptides is by acting on the phospholipid bilayer on the bacterial cell membrane, destroying the integrity of the cell membrane and forming a transmembrane channel on the cell membrane, resulting in the dissolution of the cell content and cell death

Method used

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  • Antibacterial peptide MS-CATH from Miniopterus fuliginosus and application thereof
  • Antibacterial peptide MS-CATH from Miniopterus fuliginosus and application thereof
  • Antibacterial peptide MS-CATH from Miniopterus fuliginosus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Discovery of Natural Antimicrobial Peptide MS-CATH

[0018] 1) Extraction of total RNA from long-winged bat lung tissue:

[0019] ① Take 100 mg of long-winged bat lung tissue, put it into a mortar and add liquid nitrogen to grind it into powder, transfer it to an EP tube, add 1 ml of total RNA extraction buffer (Trizol, product of Life, USA), mix well, and then place in Centrifuge at 12000rpm for 10min at 4°C.

[0020] ② Centrifuge to take the supernatant, add 0.2ml chloroform solution, mix vigorously, let stand at room temperature for 10 minutes, then centrifuge at 4°C, 12000rpm for 10 minutes, and discard the precipitate.

[0021] ③ Add an equal volume of isopropanol to the supernatant, leave it at room temperature for 10 minutes, centrifuge at 4°C, 12,000 rpm for 10 minutes, collect the precipitate, wash it once with 75% (V / V) ethanol, and dry it. The precipitate at the bottom of the tube is Wuyi Turbulence Total RNA from frog skin.

[0022] 2) Second-strand cDNA ...

Embodiment 2

[0037] Chemical Synthesis of Antimicrobial Peptide MS-CATH

[0038] (1) Synthesize the full sequence of MS-CATH with an automatic peptide synthesizer (433A, Applied Biosystems), and pass HPLC C 18 Desalting and purification by reverse phase column chromatography. (2) The molecular weight was determined by conventional matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF). (3) The purity of the purified MS-CATH was identified by high performance liquid chromatography (HPLC)>95%, and the isoelectric point determined by isoelectric focusing electrophoresis was 10.16. The amino acid sequence structure was determined to be consistent with natural MS-CATH by an automatic amino acid sequencer.

Embodiment 3

[0040] Detection of antibacterial activity of antimicrobial peptide MS-CATH

[0041] (1) Pick the test strains stored on the slope and evenly spread them on the MH solid medium (purchased from Qingdao Haibo Biotechnology Co., Ltd.) plate, and place the sterilized 0.5cm diameter filter paper on the medium On the surface, add 10 μl of 2 mg / ml MS-CATH sample solution dissolved in sterilized deionized water dropwise, incubate upside down at 37°C for 18-20 hours, and observe whether the inhibition zone is formed or not. If the sample has antibacterial activity, a clear and transparent bacteriostatic zone will be formed around the filter paper, and the larger the bacteriostatic zone, the stronger the antibacterial activity of the sample. The results showed that the antimicrobial peptide MS-CATH had antibacterial activity against the strains listed in Table 1.

[0042] (2) Determination of the Minimum Inhibitory Concentration (Minimum Inhibitory Concentration) by the 2-fold dilution...

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Abstract

The invention discloses an antibacterial peptide MS-CATH from Miniopterus fuliginosus and an application thereof in the antibacterial aspect. The antibacterial peptide MS-CATH disclosed by the invention is derived from the Miniopterus fuliginosus. A mature peptide sequence of the antibacterial peptide is obtained by searching a gene database of the Miniopterus fuliginosus and screening and analyzing, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1 in a sequence table. Through contrastive analysis, the natural antibacterial peptide MS-CATH is obviously different from the amino acid sequences of all known antibacterial peptides at present, and belongs to a novel antibacterial peptide. An antibacterial experiment result shows that the antibacterial peptide MS-CATH disclosed by the invention has extremely strong antibacterial activity on gram-positive bacteria, gram-negative bacteria and fungi, is rapid in bactericidal effect, has the characteristic of low hemolytic activity, and can be applied to preparation of drugs for resisting bacteria and inhibiting bacterial growth, preservatives, veterinary drugs, animal feeds and cosmetics.

Description

technical field [0001] The invention relates to a natural antimicrobial peptide and its application, more specifically, to a natural antimicrobial peptide MS-CATH derived from long-winged bats and its application, which belongs to the field of biomedical technology. Background technique [0002] Traditional antibiotics have achieved remarkable results in the treatment of bacterial infections. However, in recent years, with the abuse of traditional antibiotics in the fields of medicine and aquaculture, microorganisms have become more and more resistant to traditional antibiotics. Some super bacteria with strong drug resistance have been eliminated, and the problem of microbial drug resistance has become a problem that seriously threatens human health. The response to microbial resistance has always been to use new or alternative antimicrobial agents that have not been used against drug-resistant microorganisms. Drug resistance, thus requiring the continuous development of ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K38/17A61K8/64A61Q17/00A61P31/04A23L3/3526A23K20/147
CPCC07K14/47A61K8/64A61Q17/005A61P31/04A23L3/3526A23K20/147A61K38/00A23V2002/00A23V2200/10A23V2250/55
Inventor 周江罗暄巾王义鹏
Owner GUIZHOU NORMAL UNIVERSITY
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