A method for photoelectrochemical detection of miRNA-21 content based on bismuth vanadate
A miRNA-21 and photoelectrochemical technology is applied in the field of photoelectrochemical detection of miRNA-21 content based on bismuth vanadate, which can solve the problems of high experimental cost, limited practical application, complicated experimental process, etc., and achieves low cost and low detection limit. , the effect of broad application prospects
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Embodiment 1
[0041] a. BiVO 4 Preparation: Weigh 2.0g of bismuth nitrate and dissolve it in 20mL of ethylene glycol, stir magnetically until dissolved, as solution A; weigh 0.5g of ammonium metavanadate in 20mL 80°C deionized water, stir magnetically until dissolved, as solution B; Add solution B dropwise to solution A, stir for 30 minutes, transfer to an autoclave, and heat at 100°C for 15 hours; after naturally cooling to room temperature, wash with absolute ethanol and distilled water alternately until the supernatant is neutral, and dry Dry overnight at 60°C in an oven;
[0042] b. BiVO 4 Preparation of modified ITO electrode: Weigh the above-prepared BiVO 4 The powder was added to deionized water, and ultrasonically obtained a suspension of 1 mg / mL; drop-coated 30 μL BiVO on the surface of the pre-cleaned ITO electrode 4 The suspension was dried at 40°C for 2 hours for later use;
[0043] c. Biorecognition and signal amplification reaction: Mix 20 μL, 1 μM padlock probe with 10 μL...
Embodiment 2
[0048] a. BiVO 4 Preparation: Weigh 2.0g bismuth nitrate and dissolve it in 20mL glycerin aqueous solution (50v / v%), stir magnetically until it dissolves, and count it as solution A; weigh 0.8g potassium vanadate in 20mL deionized water, stir magnetically until it dissolves, and calculate Make solution B; add solution B dropwise to solution A, adjust the pH of the mixed solution to 3.0 with nitric acid, stir for 30 minutes, transfer to an autoclave, and heat at 180°C for 8 hours; after natural cooling to room temperature, absolute ethanol and Alternately wash with distilled water until the supernatant is neutral, and dry in a drying oven at 80°C overnight;
[0049] b. BiVO 4 Preparation of modified ITO electrode: Weigh the above-prepared BiVO 4 The powder was added to deionized water, and ultrasonically obtained a suspension of 1 mg / mL; drop-coated 30 μL BiVO on the surface of the pre-cleaned ITO electrode 4 The suspension was dried at 40°C for 2 hours for later use;
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