Method for enriching EPA and DHA in deep sea fish oil

A deep-sea fish oil and enrichment technology, which is applied in the direction of oil/fat refining, fat production, fermentation, etc., can solve the problems of difficult enrichment, out-of-sync enrichment, and failure to reach the maximum concentration of DHA, and achieve enrichment improvement Effect

Active Publication Date: 2020-09-11
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above two reasons make the enrichment of EPA more difficult than DHA, making the enrichment of EPA and DHA out of sync. The content

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  • Method for enriching EPA and DHA in deep sea fish oil
  • Method for enriching EPA and DHA in deep sea fish oil
  • Method for enriching EPA and DHA in deep sea fish oil

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Embodiment 1

[0020] Embodiment one: the method for EPA and DHA in a kind of enrichment deep-sea fish oil provided by the present invention, the Rhizopus oryzae lipase of 3 weight parts is dissolved in the Tris-HCl damping fluid (0.2mol / L, pH= 7.0), add 5 parts by weight of silicon dioxide particles with a particle size of 45 μm, and prepare a dispersion containing lipase and silicon dioxide after magnetic stirring for 5 minutes. Add 100 parts by weight of deep-sea fish oil to the dispersion, fill the reaction vessel with nitrogen and seal it, the reaction temperature is 40°C, and magnetic stirring is continued during the reaction process, the reaction time is 1-10h, after the reaction is completed, add 400 parts by weight of hydroxide Potassium solution (2mol / L) and 300 parts by weight of n-hexane, fully shaken, centrifuged at 5000g for 30min, transferred the upper layer solution to a new container, blown with nitrogen to remove n-hexane, and the remaining lipid is the product enriched in E...

Embodiment 2

[0022] Embodiment two: the method for EPA and DHA in a kind of enrichment deep sea fish oil provided by the present invention, the Rhizopus oryzae lipase of 3 weight parts is dissolved in the Tris-HCl damping fluid (0.2mol / L, pH= 7.0), add 5 parts by weight of silicon dioxide particles, the particle size of which is 10-20nm, and stir magnetically for 5 minutes to prepare a dispersion containing lipase and silicon dioxide. Add 100 parts by weight of deep-sea fish oil to the dispersion, fill the reaction vessel with nitrogen and seal it, the reaction temperature is 40°C, and magnetic stirring is continued during the reaction process, the reaction time is 1-10h, after the reaction is completed, add 400 parts by weight of hydroxide Potassium solution (2mol / L) and 300 parts by weight of n-hexane, fully shaken, centrifuged at 5000g for 30min, transferred the upper layer solution to a new container, blown with nitrogen to remove n-hexane, and the remaining lipid is the product enriche...

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Abstract

The invention provides a method for enriching EPA and DHA in deep sea fish oil. The method comprises the following steps: (1) dissolving sn-1,3 selective lipase in a Tris-HCl buffer solution, and adding acyl transfer accelerant silicon dioxide particles into the mixture; (2) adding deep sea fish oil into the mixture, and performing constant magnetic stirring in the reacting process; and (3) addinga potassium hydroxide solution and n-hexane, performing sufficient oscillation and centrifuge, transferring and collecting the upper solution, blowing away the n-hexane by utilizing nitrogen to obtain a residual solution, namely lipids rich in EPA and DHA. The method has the advantages that enrichment of the EPA in fish oil can be remarkably improved, even the enrichment of EPA does not reduce any more and enters a platform stage after reaching the highest content, after synthetic synthesis of lipase and acyl transfer accelerant silicon dioxide is utilized; and DHA is hardly hydrolyzed by lipase, and enzyme-catalyzed enriched DHA generally has a platform stage, so that a proper reaction time can be easily selected after the time for keeping high content of EPA is prolonged, and synchronous enrichment of the EPA and DHA can be realized.

Description

technical field [0001] The invention belongs to the technical field of enrichment methods for specific fatty acids in fats and oils, in particular to a method for enrichment of EPA and DHA in deep-sea fish oil. Background technique [0002] Many studies have confirmed that two kinds of omega-3 long-chain polyunsaturated fatty acids EPA and DHA have many benefits to human health. demand is increasing. However, the quality and quantity of deep-sea fish oil, which is the main source of EPA and DHA, have declined sharply, so the enrichment of EPA and DHA in deep-sea fish oil has attracted attention. Among the enrichment methods, lipase catalysis is the most promising method because of its mild conditions and environmental friendliness. Lipase usually has fatty acid selectivity, and it is not easy to hydrolyze long-chain polyunsaturated fatty acids EPA and DHA to keep them on the glycerol backbone, while other fatty acids (shorter chains and less unsaturated bonds) are hydrolyz...

Claims

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Application Information

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IPC IPC(8): C12P7/64C11B3/06
CPCC12P7/6472C12P7/6454C11B3/06
Inventor 曹茜廖琳萌包清彬袁永俊刘庆庆潘亚瑜
Owner XIHUA UNIV
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