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Method for increasing starch yield of duckweed mutants through induced mutation by heavy ion radiation

A technology of heavy ion radiation and mutant plants, which is applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of needing to salvage regularly, high cost of large-scale cultivation of duckweed, and restricting the development of duckweed, etc. The effect of high genetic stability

Inactive Publication Date: 2020-09-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Duckweed has high starch content and is a great potential raw material for bioenergy production. However, the high cost of large-scale cultivation of duckweed and the need for regular salvage restrict the further development of duckweed into bioenergy.

Method used

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  • Method for increasing starch yield of duckweed mutants through induced mutation by heavy ion radiation
  • Method for increasing starch yield of duckweed mutants through induced mutation by heavy ion radiation
  • Method for increasing starch yield of duckweed mutants through induced mutation by heavy ion radiation

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Effect test

Embodiment 1

[0042] In the present embodiment, a method for improving the starch production of duckweed mutant strain induced by heavy ion radiation is provided, the method is as follows: the duckweed fronds (Lemna aequinoctialis 6002) in the exponential growth phase are tiled on the MS induction medium Above, cultured in the dark for 7 weeks, transferred to a new MS induction medium, continued to culture in the dark for 5 weeks, and obtained light yellow spherical callus as the starting material for radiation mutagenesis.

[0043] Put the duckweed callus under the vertical irradiation terminal device of the heavy ion accelerator to carry out carbon heavy ion radiation mutagenesis, and the mutagenic energy heavy ion beam current is 12 C 6+ , the energy is 80MeV / u. Dose ranges were set at 10 Gy, 25 Gy, 50 Gy, and 75 Gy.

[0044] The callus after the radiation mutagenesis is differentiated and cultured into regenerated plants, and the regenerated plants are obtained and continuously cultur...

Embodiment 2

[0074] In this embodiment, a method for mutagenizing duckweed mutants with high starch yield based on heavy ion radiation is provided, the method is as follows: the duckweed fronds in the exponential growth phase are spread on the MS induction medium, and cultured in the dark for 6 week, transferred to a new MS induction medium, and continued to culture in the dark for 6 weeks to obtain light yellow spherical callus as the starting material for radiation mutagenesis.

[0075] Put the duckweed callus under the vertical irradiation terminal device of the heavy ion accelerator to carry out carbon heavy ion radiation mutagenesis, and the mutagenic energy heavy ion beam current is 12 C 6+ , the energy is 80MeV / u. Dose ranges were set at 10 Gy, 25 Gy, 50 Gy, and 75 Gy.

[0076] The callus after the radiation mutagenesis is differentiated and cultured into regenerated plants, and the regenerated plants are obtained and continuously cultured for several generations and the high-star...

Embodiment 3

[0083] In this embodiment, a method for mutagenizing duckweed mutants with high starch yield based on heavy ion radiation is provided, the method is as follows: the duckweed fronds in the exponential growth phase are spread on the MS induction medium, and cultured in the dark for 7 week, transferred to a new MS induction medium, and continued to culture in the dark for 6 weeks to obtain light yellow spherical callus as the starting material for radiation mutagenesis.

[0084] Put the duckweed callus under the vertical irradiation terminal device of the heavy ion accelerator to carry out carbon heavy ion radiation mutagenesis, and the mutagenic energy heavy ion beam current is 12 C 6+ , the energy is 80MeV / u. Dose ranges were set at 10 Gy, 25 Gy, 50 Gy, and 75 Gy.

[0085] The callus after the radiation mutagenesis is differentiated and cultured into regenerated plants, and the regenerated plants are obtained and continuously cultured for several generations and the high-star...

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Abstract

The invention specifically relates to a method for increasing the starch yield of duckweed mutants through induced mutation by heavy ion radiation. Duckweed is an ideal plant material for starch production. In order to further improve the starch production efficiency of duckweed, the invention provides a method for increasing the starch yield of duckweed mutants through induced mutation by heavy ion radiation. The method comprises the specific steps: performing induced mutation on duckweed callus through heavy ion radiation, performing differentiation culture so as to obtain regenerated plants, screening plants with high-yield starch, and performing combination with certain culture conditions so as to achieve further increase of the starch yield. The starch yield of one mutant with a goodeffect is significantly increased compared to the starch yield of a control group. As an energy plant, the accumulation amount of biomass is crucial for production of bioethanol, the mass per mutant is increased by 13.5% compared with the mass of a wild type, and the biomass per unit area is increased by 74.0% compared with that of the wild type; and the mutants which are obtained by using the method have good genetic stability of traits, and are ideal engineering strains for production of bio-energy.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for increasing the starch yield of duckweed mutant strain induced by heavy ion radiation. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] With the continuous progress of modern society, human demand for fossil fuels such as coal, oil and natural gas is increasing day by day. The combustion of these fossil fuels will release a large amount of harmful gases and solid particles, which seriously threaten human health. Biomass energy is a promising clean energy, including bioethanol, biogas and hydrogen, etc. Due to its renewable and environmentally friendly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00A01H1/04
CPCA01H1/04A01H1/06A01H4/001A01H4/008
Inventor 周功克刘宇孔英珍于昌江
Owner QINGDAO AGRI UNIV
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