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Application of taxifolin and kaempferol in preparation of livestock cryopreservation agent

A cryopreservative, taxifolin technology, applied in the application, preservation of human or animal bodies, medical preparations containing active ingredients, etc., can solve the problems of low conception rate, abnormal sperm, low sperm recovery rate, etc. Achieve the effect of maintaining fertilization ability, easy promotion and reliable effect

Active Publication Date: 2020-09-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after freezing and thawing, 40%-50% of the sperm are still inactive, which seriously reduces the utilization rate of the bulls.
However, pig sperm and sheep sperm are more sensitive to freezing stimulation. After thawing, the sperm recovery rate is low, there are many abnormal sperm, and the conception rate is lower than the normal conception rate. Therefore, the freezing of pig sperm and sheep sperm is still in the experimental stage.

Method used

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  • Application of taxifolin and kaempferol in preparation of livestock cryopreservation agent
  • Application of taxifolin and kaempferol in preparation of livestock cryopreservation agent
  • Application of taxifolin and kaempferol in preparation of livestock cryopreservation agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of pig semen cryoprotectant of the present invention comprises the steps:

[0022] Step 1, prepare the frozen base solution: accurately measure 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate, dissolve them in 100ml double distilled water, adjust the pH to 6.21, and adjust the osmotic pressure 286mosm / L, prepared as frozen base fluid;

[0023] Step 2, accurately measure 80ml of freezing base liquid, add fresh egg yolk 20ml, taxifolin 0.16mmol, kaempferol 10mg, and mix to obtain a kind of livestock semen cryopreservative (I liquid) of the present invention;

[0024] Get the above-mentioned livestock semen cryoprotectant (I liquid) of 94ml and add 6ml glycerin, filter sterilization, cool to room temperature, can obtain another kind of livestock semen cryopreservation agent (II liquid) of the present invention and be used for preserving pig semen, put Put it in a refrigerator at 4°C ...

Embodiment 2

[0045] The preparation method of goat semen cryopreservation agent of the present invention, comprises the steps:

[0046] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0047] Step 2, accurately measure 80ml of frozen base liquid, add 20ml of fresh egg yolk, taxifolin 0.08mmol, and kaempferol 30mg, and mix well to prepare a cryopreservative for livestock semen (I liquid) of the present invention;

[0048] Get the above-mentioned livestock semen cryopreservation agent (I liquid) of 97ml and add 3ml glycerin to be mixed with the mixed solution of 100ml, filter sterilize, cool to room temperature, namely obtain another kind of livestock semen cryopreservation agent (II liquid) of the present invention for use in Save the goat semen and put it in a refrigerator at 4°C for the following experiments.

[0049] Control group 21-23:

[0050] The difference from Example 2 is that taxifolin and kaempferol were not added to control group 21; taxifolin w...

Embodiment 3

[0070] The preparation method of bovine semen cryopreservation agent of the present invention, comprises the steps:

[0071] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0072] Step 2: Accurately measure 80ml of frozen base solution, add 20ml of fresh egg yolk, taxifolin 0.14mmol, and 25mg of kaempferol to obtain a livestock semen cryopreservative (I liquid) of the present invention.

[0073] Get 96ml of the above-mentioned livestock semen cryopreservative and add 4ml glycerin to be mixed with 100ml of mixed solution, mix well, filter and sterilize, and cool to room temperature to obtain another livestock semen cryopreservative (II liquid) of the present invention for preservation The bovine semen was placed in a refrigerator at 4°C for the following tests.

[0074] Control group 31-33:

[0075] Different from Example 3, the control group 31 did not add taxifolin and kaempferol; the control group 32 did not add taxifolin; the control group ...

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Abstract

The invention discloses application of taxifolin and kaempferol in preparation of a livestock cryopreservation agent. Meanwhile, the invention discloses a livestock cryopreservation agent containing taxifolin, kaempferol and a freezing base solution. According to the invention, taxifolin and kaempferol are combined for cryopreservation of semen of pigs, sheep and cattle for the first time; the application is reliable in effect, easy to popularize and suitable for artificial insemination of pig, sheep and cattle tubule frozen semen, long-term storage of the semen after ultralow-temperature freezing and semen deposition after long-distance transportation, the sperm motility rate, acrosome integrity rate and plasma membrane integrity rate of the frozen-thawed pig, sheep and cattle can be remarkably increased, and the fertilization capacity of sperms is kept.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation of mammalian semen, and specifically relates to the application of taxifolin and kaempferol as an antifreeze protective agent in cryopreservation of livestock semen. Background technique [0002] Sperm freezing technology is of great significance. In theory, it can store semen permanently, realize the establishment of semen gene bank, exchange germplasm resources globally, improve the utilization rate of high-quality breeding pigs, and effectively prevent the spread of diseases. The sustainable development of animal husbandry and the sufficient supply of animal products in my country and the world have important practical significance. [0003] Sperm are sensitive to temperature changes. In the process of freezing and cooling, sperm are susceptible to freezing damage, mainly including physical damage, chemical damage and oxidative damage. Compared with fresh semen, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A61K31/352A61P39/06
CPCA01N1/0221A01N1/0226A61K31/352A61P39/06
Inventor 李青旺刘定帮杨丽吴琳
Owner NORTHWEST A & F UNIV
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