Tortoise small peptide as well as preparation method and application thereof

A preparation-type, tortoise shell technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems that cannot be artificially synthesized, complex components, low purity, etc., and achieve improved sleep health food and stable physical and chemical properties , the effect of reducing production costs

Active Publication Date: 2020-09-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of tortoise peptide often adopts proteolysis method. Although the yield of the obtained peptide product is high, the purity is not high, and due to the complex composition, it cannot be artificially synthesized.

Method used

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  • Tortoise small peptide as well as preparation method and application thereof
  • Tortoise small peptide as well as preparation method and application thereof
  • Tortoise small peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Extraction of peptides

[0033] (1) After the stone turtle shell is broken, soak it with 4% volume concentration of acetic acid for 1 hour, then decoct it with water, filter it, collect the decoction solution and concentrate it, and then extract the concentrated solution with petroleum ether to remove fat-soluble impurities, and then The aqueous phase was lyophilized to obtain protein extracts.

[0034] (3) Add the protein extract freeze-dried powder to the NaOH solution with pH 7.5 according to the solid-to-liquid ratio of 1:25 (g / mL), then add alkaline protease according to 1.2% of the weight of the NaOH solution, under the condition of 55°C water bath Enzymolysis was carried out for 6 hours, and then boiled to inactivate the enzyme, filtered, the filtrate was concentrated and freeze-dried to obtain the polypeptide.

[0035] The main components of the final product were determined according to national standards, and the results are shown in Table 1.

[0036] Tab...

Embodiment 2

[0053] Example 2 Animal experiments evaluating the sleep-promoting effect of different polypeptide fragment monomers

[0054] SPF grade Kunming male mice with a body weight of 18±2g were selected. The animal laboratory is SPF clean grade, and the feeding conditions are ambient temperature 25±1°C, humidity 60±10%, light / darkness 12 / 12, free access to food and water. After 3 days of adaptive feeding, the experimental animals were divided into: blank control group, W-G-X-B group, W-G-P-G-B group, W-W-Y-W-R-Hex group, W-R-W-X-H-T-H-N-W group, W-N-A-R-W-P-G-J group, with 10 mice in each group. Under the condition of ensuring normal diet and drinking water, all mice were deprived of food at 8 o'clock every morning, and gavage began at 9 o'clock. The blank group was fed with 0.9% normal saline, and the mice in the four polypeptide segment monomer groups were fed with 50 mg / kg·d corresponding polypeptide monomer sample solution for 14 consecutive days. Two hours after the end of gav...

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Abstract

The invention discloses a tortoise small peptide. The tortoise small peptide has any one of the following amino acid sequences: W-W-Y-W-R-Hex; W-G-P-G-B; W-R-W-X-H-T-H-N-W. The invention further discloses a preparation method and application of the tortoise small peptide. The tortoise small peptide can significantly shorten the sleep latency period of mice and prolong the sleep time, has a good sleep promoting effect, is clear in structural composition, stable in physical and chemical properties, more controllable in quality and easier to guarantee in efficacy compared with existing polypeptides, can be prepared by a synthesis method, and is beneficial to reducing resource waste and lowering the production cost.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to a tortoise small peptide with sleep-promoting effect and a preparation method thereof. Background technique [0002] Insomnia symptoms are becoming more and more common in the population, and lack of sleep can lead to very serious health problems, such as depression, heart disease, high blood pressure and obesity. Although benzodiazepine receptor sedative-hypnotic drugs are widely used to induce sleep, the side effects of these drugs are very obvious, such as cognitive decline, memory loss, and long-term use can lead to tolerance and addiction. Over the past few decades, sleep regulation has become a worldwide problem. People urgently need safer and more effective hypnotic and sedative drugs. [0003] Tortoise shell has always been regarded as a high-value Chinese herbal medicine in my country. "Compendium of Materia Medica" records that tortoise shell has detoxification, ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/16C07K1/14A61K38/08A61P25/20
CPCA61K38/00A61P25/20C07K7/06C12P21/06
Inventor 张久亮王箴言吕彦帛闫佳兴
Owner HUAZHONG AGRI UNIV
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