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Peripheral blood TCR marker of lung cancer, and detection kit and application thereof

A marker and peripheral blood technology, applied in the field of genetic engineering, can solve the problems of large workload, high detection cost, and large space occupied by detection, achieve high specificity and accuracy, reduce detection cost, and low labor cost. Effect

Pending Publication Date: 2020-09-15
CHENGDU EXAB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] Exfoliated cells are detected on patients' sputum, bronchial secretions, and pleural effusion, but there are some problems: the specificity of tumor detection is high, but the sensitivity is not ideal; cancer cells are detected, and the tumor cannot be located; in order to ensure Reliability, the workload required for detection is large; extraction of pleural effusion is more painful and harmful to patients, etc.
However, nuclear magnetic resonance detection has the disadvantages of expensive equipment, large volume, large space occupation, and high detection cost.

Method used

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  • Peripheral blood TCR marker of lung cancer, and detection kit and application thereof
  • Peripheral blood TCR marker of lung cancer, and detection kit and application thereof
  • Peripheral blood TCR marker of lung cancer, and detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Through immunographic analysis, the sequence set of lung cancer TCR marker CDR3 was obtained:

[0064] 1. Sampling and immune map analysis

[0065] Collect 1301 control groups (including healthy people and patients with non-tumor diseases, 1300 people for model building, 1 healthy person for verification), 41 lung cancer patients (40 people for model building, 1 person for verification) and Peripheral blood (10mL per person) of a subject with unknown health status, through high-throughput sequencing to obtain the amino acid sequence of the epitope 3 (CDR3) of the TCR of the subject and the control group, to ensure the functional TCR of each sample The total number of CDR3 sequences should not be less than 30,000;

[0066] 2. The CDR3 sequence of the TCR of each sample is randomly sampled without replacement, so that the total number of CDR3 sequences of each sample is 30,000. For any specific CDR3 sequence X, the number of repeated occurrences in the single-sample seq...

Embodiment 2

[0073] Validate the specificity of the lung cancer TCR marker CDR3 sequence set:

[0074] 1. Sampling and immune map analysis

[0075] Peripheral blood (10 mL per person) was collected from 304 non-lung cancer tumor patients and 6 subjects with unknown health status, and the amino acid sequence of the TCR epitope 3 (CDR3) of the subjects and the control group was obtained by high-throughput sequencing , to ensure that the total number of functional TCR CDR3 sequences of each sample is not less than 30,000; random non-replacement sampling is performed on the TCR CDR3 sequences of each sample, so that the total number of CDR3 sequences of each sample is 30,000.

[0076] 2. Randomly select 100 healthy people and 45 non-tumor disease patients from the control group in Example 1.

[0077] 3. According to the immune profiles of 100 healthy people, 45 non-tumor disease patients, and 40 lung cancer patients from Example 1, and 304 non-lung cancer tumor patients and 6 subjects with un...

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Abstract

The invention discloses a peripheral blood TCR marker of a lung cancer, and a detection kit and an application thereof. The marker comprises at least one of proteins as shown in a sequence SpecSeq1-100. Based on a high-throughput sequencing method, only a small amount of peripheral blood needs to be adopted, RNA needs to be extracted, an immune map library is established by processing a sample, acharacteristic TCR sequence in the lung cancer peripheral blood is determined firstly through high-throughput sequencing and TCR data analysis, and then a test result of a sample to be tested is compared with the characteristic TCR sequence so that whether a lung cancer exists or not is determined. The marker, the kit and the application can be used to simultaneously compare a huge number of lungcancer specific TCR sequences, have higher specificity and accuracy compared with single detection of one or more markers, and improve diagnosis efficiency.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a peripheral blood TCR marker of lung cancer, a detection kit and application thereof. Background technique [0002] According to the latest statistics from the World Health Organization in 2018, lung cancer is the most common malignant tumor in the world, ranking first in the incidence of various cancers (11.6%, tied with female breast cancer), and ranking first in the proportion of cancer deaths (18.4%). East Asia is one of the regions with the highest incidence of male lung cancer. The incidence of lung cancer in China is about 40 / 100,000, which is higher than the world average (31.5 / 100,000). In China, lung cancer is the malignant tumor with the highest incidence rate among men, and it is also the malignant tumor with the highest death rate (both men and women). Although there are many treatments for lung cancer, the actual benefits for patients are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A61K39/00A61P35/00
CPCG01N33/57423G01N33/57488A61K39/001111A61P35/00G01N2333/7051A61K2039/86
Inventor 张志新杨鑫卓越钟雪梅治想林
Owner CHENGDU EXAB BIOTECH CO LTD
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