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Antibacterial composition and application thereof to preparation of staphylococcus epidermidis quorum sensing inhibitor

A technology of staphylococcus epidermidis and antibacterial composition, applied in the field of medicine, can solve the problems that cannot satisfy consumers' pursuit of health, greenness and sustainability, troubled antibacterial treatment, and reduced ability to treat skin and soft tissue infections, achieving important development value and significance , Significant preventive effect, good inhibitory effect

Inactive Publication Date: 2020-09-18
广东华夏友美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the problem of antibiotic resistance has been one of the important factors that plague antibacterial treatment, and drug-resistant Staphylococcus epidermidis infection may lead to more serious diseases and consequences
[0007] With the widespread clinical application of broad-spectrum antibacterial drugs, the problem of drug resistance of opportunistic pathogenic bacteria is becoming more and more serious, which greatly reduces the ability of commonly used antibacterial drugs to treat skin and soft tissue infections caused by bacteria, which can no longer satisfy consumers in pursuit of health. , green and sustainable aspirations

Method used

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  • Antibacterial composition and application thereof to preparation of staphylococcus epidermidis quorum sensing inhibitor
  • Antibacterial composition and application thereof to preparation of staphylococcus epidermidis quorum sensing inhibitor
  • Antibacterial composition and application thereof to preparation of staphylococcus epidermidis quorum sensing inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 antibacterial composition in vitro antibacterial activity screening

[0048] 1. Experimental materials and instruments

[0049] ① Reagents: alkaloid (Chengdu Puruifa Technology Development Co., Ltd., CAS No.: 28808-62-0, purity ≥ 98.0%, batch number: PRF8041042), catacrine (Chengdu Puruifa Technology Development Co., Ltd., CAS No.: 484-29-7, purity ≥98.0%, batch number: 15032311), Phellodendron (Chengdu Prefa Technology Development Co., Ltd., CAS No.: 751-03-1, purity ≥98.0%, batch number: 15032605); tryptone soybean Broth granules (Guangdong Huankai Microbial Technology Co., Ltd., batch number: 1063863); Fusidic acid (Shanghai Yuanye Biotechnology Co., Ltd., batch number: Z19J6Q1, purity > 99%); PBS buffer solution (U.S. Hyclone Company, batch number: A20181004).

[0050] ②Instruments: SW-CJ-1F ultra-clean bench (Sujing Group Antai Company); Memmert constant temperature incubator (Shanghai Yiheng Scientific Instrument Co., Ltd.); YSQ-LS-18SI portable pre...

Embodiment 2

[0070]Example 2 Different ratios of antibacterial compositions screened for antibacterial activity against drug-resistant Staphylococcus epidermidis

[0071] 1. Experimental materials and instruments

[0072] ① Reagents: alkaloid (Chengdu Puruifa Technology Development Co., Ltd., CAS No.: 28808-62-0, purity ≥ 98.0%, batch number: PRF8041042), catacrine (Chengdu Puruifa Technology Development Co., Ltd., CAS No.: 484-29-7, purity ≥98.0%, batch number: 15032311), Phellodendron (Chengdu Prefa Technology Development Co., Ltd., CAS No.: 751-03-1, purity ≥98.0%, batch number: 15032605); tryptone soybean Broth granules (Guangdong Huankai Microbial Technology Co., Ltd., batch number: 1063863); Fusidic acid (Shanghai Yuanye Biotechnology Co., Ltd., batch number: Z19J6Q1, purity > 99%); PBS buffer solution (U.S. Hyclone Company, batch number: A20181004).

[0073] ②Instruments: SW-CJ-1F ultra-clean bench (Sujing Group Antai Company); Memmert constant temperature incubator (Shanghai Yihe...

Embodiment 3

[0092] Embodiment 3: antibacterial composition is to the inhibitory action of drug-resistant Staphylococcus epidermidis biofilm

[0093] 1. Experimental materials and instruments

[0094] ① Reagents: alkaloid (Chengdu Puruifa Technology Development Co., Ltd., CAS No.: 28808-62-0, purity ≥ 98.0%, batch number: PRF8041042), catacrine (Chengdu Puruifa Technology Development Co., Ltd., CAS No.: 484-29-7, purity ≥98.0%, batch number: 15032311), Phellodendron (Chengdu Prefa Technology Development Co., Ltd., CAS No.: 751-03-1, purity ≥98.0%, batch number: 15032605); tryptone soybean Broth granules (Guangdong Huankai Microbial Technology Co., Ltd., batch number: 1063863); CCK-8 kit (New Saimei Biological Co., Ltd., batch number: C6055); Gluta fixative (special for electron microscopy, 2.5%) (US Solarbio company, Batch number: 20181201); absolute ethanol (Tianjin Zhiyuan Chemical Reagent Co., Ltd., batch number: 2016120149); butanol (Tianjin Zhiyuan Chemical Reagent Co., Ltd., batch n...

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Abstract

The invention discloses an antibacterial composition which comprises fraxinellone, dictamnine and obakunone. Meanwhile, the invention provides application of the antibacterial composition to preparation of a staphylococcus epidermidis inhibitor, application of the antibacterial composition to preparation of a medicine for treating and / or preventing related skin soft-tissue infection diseases caused by excessive proliferation of staphylococcus epidermidis, and application of the antibacterial composition to preparation of a flora quorum sensing system inhibitor. The antibacterial composition has an excellent antibacterial effect on staphylococcus epidermidis and particularly drug-resistant staphylococcus epidermidis; and the mechanism of the antibacterial composition is that by inhibiting expression of a quorum sensing system key gene sarA mediated by autoinducing peptides (AIP) and a quorum sensing system key gene luxS mediated by LuxS / AI-2, a pathogenic flora quorum sensing system isinhibited, so as to inhibit formation of bacterial biofilms on the surfaces of thalli. Components of the antibacterial composition are from natural traditional Chinese medicine plants; and the antibacterial composition has the advantages of no toxicity, no stimulation and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to an antibacterial composition and its application in preparing staphylococcus epidermidis quorum sensing inhibitors. Background technique [0002] Antibacterial drugs are antibacterial drugs generally refer to drugs with bactericidal or bacteriostatic activity, including various antibiotics, sulfonamides, imidazoles, nitroimidazoles, quinolones and other chemically synthesized drugs. Antibacterial drugs can inhibit and kill pathogens at a certain concentration. Due to the different antibacterial spectrum of different antibacterial drugs, and with the extensive use of antibiotics, antibiotic resistance in clinical applications is gradually emerging. In recent years, domestic and foreign countries have gradually turned their attention to the combined application of antibiotics and quorum sensing inhibitors, using quorum sensing inhibitors to destroy the quorum sensi...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61K31/4355A61K31/366A61P31/04A61P17/02
CPCA61K31/365A61K31/366A61K31/4355A61P17/02A61P31/04A61K2300/00
Inventor 沈志滨胡锡昌张衍湖邓榕榕
Owner 广东华夏友美生物科技有限公司
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