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Preparation method of proanthocyanidin polymer and proanthocyanidin polymer

A technology of proanthocyanidins and polymers, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of lack of preparation methods for proanthocyanidins polymers, achieve high degree of polymerization and mild process conditions , The effect of simple preparation process

Pending Publication Date: 2020-09-18
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects or deficiencies of the prior art, the object of the present invention is to provide a method for preparing proanthocyanidin multimers and proanthocyanidin multimers, so as to solve the problem of lack of preparation of proanthocyanidin multimers in existing methods. technical issues of the method

Method used

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  • Preparation method of proanthocyanidin polymer and proanthocyanidin polymer
  • Preparation method of proanthocyanidin polymer and proanthocyanidin polymer
  • Preparation method of proanthocyanidin polymer and proanthocyanidin polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step S1, prepare bayberry leaf (water chestnut) raw material;

[0030] Step S2, drying the bayberry leaf in an oven at 25-40°C for 12 hours, taking it out, crushing it with a pulverizer to pass through a 60-80 mesh sieve, and then freezing the bayberry leaf powder in a -20°C refrigerator.

[0031] Step S3, weighing 10g red bayberry leaf powder and adding it into a brown silk bottle with 400mL 70% acetone aqueous solution to obtain a mixed solution, adding 0.4g ascorbic acid to the obtained mixed solution to prevent oxidation during the extraction process, and shaking the obtained material solution Mix well and seal, then shake the brown silk bottle intermittently in the dark at room temperature to extract proanthocyanidin polymers, and the shaking extraction time is 12 hours.

[0032] Step S4, suction-filter the obtained mixed material liquid to obtain a filtrate, extract the filtrate with 400 mL of analytically pure chloroform at a ratio of 1:1, shake well and let stan...

Embodiment 2

[0040] Change the 10g bayberry leaf powder in embodiment 1 into 20g bayberry leaf powder; change the 70% acetone of 400mL in embodiment 1 step 3 into the 70% acetone of 800mL; the ascorbic acid that adds is 0.8g (0.1%w / v) ; The filtrate extraction in step 4 is changed into 1:1 equivolume extraction with the analytical pure chloroform of 800mL; All the other steps are with embodiment 1.

[0041] Finally, 1.090 g of myrica rubra leaf proanthocyanidin multimer was obtained, and the yield was 5.45%.

[0042] After being degraded by phloroglucinol-sulfuric acid method, it is detected by RP-HPLC-DAD, and the detection results are as follows: figure 2 As shown, the average degree of polymerization of bayberry leaf proanthocyanidin multimer is 29.8.

Embodiment 3

[0044] Change the 10g bayberry leaf powder in embodiment 1 into 20g bayberry leaf powder; change the 70% acetone of 400mL in embodiment 1 step 3 into the 70% acetone of 800mL; the ascorbic acid that adds is 0.8g (0.1%w / v) ; The filtrate extraction in step 4 was changed to 1:1 equal-volume extraction with 800mL of analytically pure chloroform; extracted twice during the shaking extraction time of 12h; the remaining steps were the same as in Example 1.

[0045] The final proanthocyanidin multimer of bayberry leaf was 1.104g, and the yield was 5.52%.

[0046] After being degraded by the phloroglucinol-sulfuric acid method, the average degree of polymerization of the above-mentioned bayberry leaf proanthocyanidin multimer was 23.8 as detected by RP-HPLC-DAD.

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Abstract

The invention discloses a preparation method of a proanthocyanidin polymer. The preparation method comprises the following steps: preparing a raw material, which is waxberry leaves or grape seeds; preprocessing the raw material to obtain raw material powder; mixing the raw material powder with an acetone water solution to obtain a mixed solution, then adding ascorbic acid into the mixed solution,and carrying out lightproof intermittent oscillation extraction to obtain a mixed feed liquid; performing suction filtration on the obtained mixed feed liquid to obtain filtrate, mixing the filtrate with trichloromethane, and collecting supernatant liquid; adding sodium chloride into the collected supernatant liquid, centrifuging to collect a coarse precipitate; washing the coarse precipitate withwater, and carrying out freeze drying to obtain the proanthocyanidin polymer. The preparation process is simple, the process conditions are mild, the polymer yield is high, the polymerization degreeis high, and the prepared proanthocyanidin polymer can be used for proanthocyanidin structure and biological activity analysis and application, and can also be used for preparing proanthocyanidin oligomers or metal chelating agents.

Description

technical field [0001] The invention belongs to the technical field of natural product preparation and relates to food phytochemical components, in particular to a preparation method of a proanthocyanidin multimer and the proanthocyanidin multimer. Background technique [0002] Proanthocyanidins are the most abundant expression form of polyphenols in the plant kingdom except lignin, and are also the most abundant and sufficient flavonoids in human diet. They exist in large quantities in plants, mainly in plant epidermis, fruit peels, seeds, etc. place. [0003] Both bayberry fruit and grapes have extremely high added value. Its economic cultivation in China has a history of many years and its industrialization has been relatively mature. However, compared with bayberry fruit and grape pulp, the utilization of bayberry leaves and grape seeds is extremely low. Research has found that the content of proanthocyanidins in bayberry leaves is very high, and its structure is mainly...

Claims

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Application Information

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IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 杨海花姜坤叶焕峰武永杰
Owner NORTHWEST A & F UNIV
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