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Application of corosolic acid and derivatives thereof in preparation of medicines for resisting eimeria tenella

A technology for Eimeria and tenderness, which is applied in the field of biomedicine, can solve the problem of no corosolic acid anti-Eimeria tenenia, and achieve the effect of promoting development and utilization

Inactive Publication Date: 2020-09-18
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the anti-Eimeria tenella of corosolic acid, and the known properties of corosolic acid cannot be used to infer whether it has an anti-Eimeria tenella effect

Method used

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  • Application of corosolic acid and derivatives thereof in preparation of medicines for resisting eimeria tenella
  • Application of corosolic acid and derivatives thereof in preparation of medicines for resisting eimeria tenella
  • Application of corosolic acid and derivatives thereof in preparation of medicines for resisting eimeria tenella

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Experimental program
Comparison scheme
Effect test

Embodiment 1E

[0039] Anticoccidial effect evaluation in embodiment 1E.tenella MDBK cell culture model

[0040] The E.tenella MDBK cell culture model was used to evaluate the anti-coccidian effect, and the specific test steps were as follows:

[0041] Digest the MDBK cells grown until the fusion state reaches more than 80% with 0.25% trypsin digestion solution, and use 4×10 5 Plate 12-well cell culture plates at the concentration of cells per well and place at 37°C with 5% CO 2 cultured in an incubator for 24 h.

[0042] When the cells in the 12-well culture plate grow to reach 80% or above, inoculate 10×10 4 Sporozoites / ml, discard the medium after 4 hours, wash 3 times with PBS, wash off the suspended sporozoites, add MEM medium with 5% fetal bovine serum to each well, add the drug to be tested, and set 3 repetitions, at the same time Set up blank and positive controls. Place at 37°C with 5% CO 2 continue to grow in the incubator.

[0043] After culturing for 48 h, the culture medium...

Embodiment 2

[0075] Embodiment 2 animal experiments

[0076] Experimental Materials:

[0077] Preparation of corosolic acid powder: take 10 g of corosolic acid (content not less than 99%) and mix with 90 g of starch.

[0078] Control drugs: conventional anticoccidial drug Coccidian powder (30% sodium sulfachlorpyrazine), produced by Shanghai Novartis Animal Health Co., Ltd.; diclazuril, produced by Zhejiang Huineng Animal Pharmaceutical Co., Ltd.

[0079] Coccidia oocysts: sporulated oocysts of Eimeria tenella (E. tenella) Guangdong strain, preserved by the Institute of Animal Health, Guangdong Academy of Agricultural Sciences, and rejuvenated in coccidia-free chicks before use.

[0080] Chicks: Lingnan yellow broiler chickens, provided by the Institute of Animal Science, Guangdong Academy of Agricultural Sciences, were raised in a sterilized special animal room; the chicken cages and utensils were strictly sterilized, free to eat and drink pure water; before the experiment, the chicks we...

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Abstract

The invention discloses application of corosolic acid and derivatives thereof in the preparation of medicines for resisting eimeria tenella. Cell level tests and animal tests are carried out to test the effect of corosolic acid on resisting eimeria tenella infection, and results show that corosolic acid has a remarkable eimeria tenella inhibiting effect, the insect resisting index of corosolic acid reaches 177.5, and the insect resisting effect of corosolic acid is remarkably superior to that of conventional insect resisting medicines including sulfachloropyrazine sodium and diclazuril. The invention discloses new application of corosolic acid in treatment of eimeria tenella infection, development and application of corosolic acid are promoted, and development and utilization of new drugsfor resisting eimeria tenella are also promoted.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of corosolic acid and its derivatives in the preparation of drugs against Eimeria tenella. Background technique [0002] Coccidiosis is a parasitic disease caused by infection with one or more species of Eimeria spp. At present, coccidiosis has become one of the most common, most prone to and serious diseases in intensive farms, with an incidence rate as high as 50% to 70% and a mortality rate of 20% to 30%. Under the circumstances that medical conditions do not allow, its prevalence rate can be as high as 80%. Coccidiosis will affect the commercial production performance of the host, such as a decrease in weight gain rate, a decrease in feed remuneration, and a decrease in egg production of laying hens. Eimeria tenella (E. tenella) is one of the most pathogenic pathogens in Eimeria coccidiosis, which parasitizes in chicken cecum, also known as cecal coccidia. Suffer...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/56A61P33/02
CPCA61K31/56A61P33/02C07J63/008
Inventor 廖申权孙铭飞戚南山吕敏娜吴彩艳李娟林栩慧胡俊菁蔡海明肖文婉于林增张健騑谢明权
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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