Mixed nanostructure substrate, preparation method and application thereof

Abstract

The invention relates to a mixed nanostructure substrate, a preparation method and application thereof. The mixed nanostructure substrate comprises a substrate layer; and nanospheres which are distributed at intervals and are formed above the substrate layer; the upper surface of each nanosphere is provided with a plurality of concave parts; and each concave part contains a metal nanoparticle aggregate. By combining the Raman enhancement effect of the metal nanoparticle aggregate in each concave part and the plurality of nanospheres, the enhancement factor is up to 108, and the sensitivity andthe detection precision are relatively high; meanwhile, the sizes and the intervals of the nanospheres are controllable, the positions and the intervals of the concave parts on each nanosphere are controllable, the morphology and the distribution uniformity of nano particle aggregation can be controlled by controlling reaction conditions, uniform distribution and controllable distribution of hotspots are achieved, and high stability is guaranteed. The substrate has the advantages of high enhanced Raman activity, high uniformity, excellent stability, batch reproducibility and the like.

Description

Technical field [0001] The present disclosure belongs to surface-enhanced Raman technology, and relates to a hybrid nanostructure substrate, a preparation method and an application thereof. Background technique [0002] Surface enhanced Raman technology uses the plasmon resonance between the metal nanostructure surface and the metal surface to induce enhanced scattering. It has the advantages of high sensitivity, high selectivity and loose detection conditions, and can be widely used in single molecule detection, biomedical detection and other fields. [0003] In the early days, there were many techniques to enhance Raman signals, such as the use of electrochemically rough metal electrodes or metal nanosols to form nanoparticle agglomerates after being dried. Due to the random nature of nanostructures, the Raman signals were uneven. At present, a variety of enhanced Raman scattering structures and preparation methods have been studied, including nanowire and nanoparticle structures...

AI Technical Summary

Problems solved by technology

However, due to the coating method, it is difficult to control the distribution of silver nanowires and silver nanoparticles at each hot spot, which makes the distribution of hot spots uneven and the measured Raman enhanced signal is unstable.

[0006] Raman enhancement technology with ultra-high sensitivity has been developed for decades. Due to the limitation of high-cost Raman signal enhancement chips, the Raman enhancement substrate realized at the same time cannot have both high sensitivity and high stability. It is widely used in the detection of trace amounts of substances, so it is urgent to provide a structure that enhances Raman signals with low cost, simple manufacturing process, mass production, high detection accuracy and high stability.

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