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Semiconductor nano biosensor and preparation method thereof

A biosensor and semiconductor technology, applied in the fields of instruments, scientific instruments, electrochemical variables of materials, etc., can solve the problems of unseen detection of Staphylococcus aureus, and achieve fast detection, low cost, improved accuracy and specificity. Effect

Active Publication Date: 2020-09-22
DALIAN POLYTECHNIC UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

There are patents and literature reports that detect Staphylococcus aureus with electrochemical immunosensing, optical fiber biosensors, fluorescent sensors, DNA sensors, etc., but there is no use of photoelectrochemical aptamer sensors based on semiconductor nanomaterials to detect Staphylococcus aureus to report

Method used

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  • Semiconductor nano biosensor and preparation method thereof
  • Semiconductor nano biosensor and preparation method thereof
  • Semiconductor nano biosensor and preparation method thereof

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. like figure 1 As shown, the construction process of the working electrode of the semiconductor nano-biosensor of the present invention includes sequentially modifying the rare earth-doped Bi on the surface of the ITO electrode. 2 MoO 6 Nanoparticles, Ag 2 S, Au, Aptamer, BSA, FPB (this specific embodiment is example with Staphylococcus aureus), obtain semiconductor nano biosensor working electrode, concrete steps are as follows:

[0034] Step 1, preparation of Er / Tm / Yb co-doped Bi 2 MoO 6 Nanoparticles

[0035] Will have weighed Er 2 o 3 、Tm 2 o 3 , Yb 2 o 3 Dissolve it in 50mL of dilute nitric acid solution (1mol / L) under the condition of heating in a water bath at 90°C to obtain a nitrate solution. After it is completely dissolved and cooled to room temperature, it is recorded as solution A, Er 3+ 、Tm 3+ , Yb 3+ The doping concent...

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Abstract

The invention discloses a preparation method of a semiconductor nano biosensor. The method comprises the steps: putting the prepared Er / Tm / Yb-Bi2MoO6 nanoparticles into water and carrying out ultrasonic treatment to obtain uniformly mixed turbid liquid to be modified on on ITO conductive glass, calcining in a muffle furnace, cooling to room temperature, depositing Ag2S on the BMO-1 / ITO electrode through a continuous ion layer adsorption reaction, and modifying nano gold ions; then dropwise coating a food-borne pathogenic bacteria aptamer solution, and incubating overnight at 4 DEG C; continuously dropwise adding BSA to block non-specificity, and finally dropwise adding food-borne pathogenic bacteria to successfully construct a working electrode; respectively putting a platinum wire electrode serving as a counter electrode and a saturated calomel electrode serving as an auxiliary electrode into a PBS solution to construct a three-electrode system, AA serving as an electron donor and a xenon lamp serving as a light source to successfully construct the semiconductor nano biosensor for detecting the food-borne pathogenic bacteria. The sensor has the advantages of short detection time,high specificity, low cost, potential portability and the like.

Description

technical field [0001] The invention belongs to the field of food safety detection, and refers to a semiconductor nanometer biosensor for detecting food-borne pathogenic bacteria and a preparation method thereof. Background technique [0002] Worldwide, as people's dependence on processed foods continues to increase, the number of global foodborne diseases is increasing year by year. According to statistics, hundreds of millions of people in the world get sick every year due to food contamination. Among them, the number of patients caused by microbial pollution ranks first, and it is the most prominent health and hygiene problem in the world at present. In recent years, the proportion of foodborne diseases in my country has been on the rise, and about one sixth of the population suffers from foodborne diseases. Therefore, food poisoning and food-borne diseases caused by food-borne pathogens have become the primary problems facing food safety in China. [0003] There are d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/26
CPCG01N27/30G01N27/3278G01N27/26
Inventor 郝洪顺李雪莲朱良良丁超侯红漫张公亮毕景然闫爽
Owner DALIAN POLYTECHNIC UNIVERSITY
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