Peripheral blood TCR marker sequence of colorectal cancer as well as detection kit and application of peripheral blood TCR marker sequence

A marker and colorectal cancer technology, applied in the field of genetic engineering, can solve the problems that cannot be used as screening and early diagnosis criteria, cannot obtain lesion histopathological examination, colorectal cancer lacks sensitivity and specificity, etc., to reduce detection costs, The effect of low labor cost and simple sampling

Pending Publication Date: 2020-09-22
CHENGDU EXAB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 3) CT virtual colonoscopy: non-invasive, relatively simple and safe, suitable for patients who cannot undergo colonoscopy, especially those with intestinal stenosis caused by malignant tumors, but cannot obtain lesion tissue for pathological examination
On the one hand, because colorectal cancer has no obvious symptoms in the early stage, or the symptoms are similar to lower gastrointestinal diseases such as hemorrhoids and gastroenteritis, many patients will miss the opportunity of early detection of cancer due to reasons such as resistance to digital rectal examination and colonoscopy requiring anesthesia
On the other hand, according to research in the United States, known tumor markers lack sensitivity and specificity for colorectal cancer and cannot be used as screening and early diagnosis criteria

Method used

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  • Peripheral blood TCR marker sequence of colorectal cancer as well as detection kit and application of peripheral blood TCR marker sequence
  • Peripheral blood TCR marker sequence of colorectal cancer as well as detection kit and application of peripheral blood TCR marker sequence
  • Peripheral blood TCR marker sequence of colorectal cancer as well as detection kit and application of peripheral blood TCR marker sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Through immunographic analysis, the sequence set of colorectal cancer TCR marker CDR3 was obtained:

[0059] 1. Sampling and immune map analysis

[0060] Collect 1301 control groups (including healthy people and patients with non-tumor diseases, 1300 people for model building, 1 healthy person for verification), 101 patients with colorectal cancer (100 people for model building, 1 person for verification) and the peripheral blood of a subject with unknown health status (10mL per person), and the amino acid sequence of the TCR epitope 3 (CDR3) of the subject and the control group was obtained by high-throughput sequencing to ensure the functionality of each sample The total number of CDR3 sequences of TCR should not be less than 30,000;

[0061] 2. The CDR3 sequence of the TCR of each sample is randomly sampled without replacement, so that the total number of CDR3 sequences of each sample is 30,000. For any specific CDR3 sequence X, the number of repeated occurrences i...

Embodiment 2

[0068] Verify the specificity of the colorectal cancer TCR marker CDR3 sequence set:

[0069] 1. Sampling and immune map analysis

[0070] Peripheral blood (10 mL per person) was collected from 358 tumor patients without colorectal cancer and 3 subjects with unknown health status, and the epitope 3 (CDR3) amino acid of TCR in the subjects and the control group was obtained by high-throughput sequencing Sequences, to ensure that the total number of CDR3 sequences of functional TCRs in each sample is not less than 30,000; random non-replacement sampling is performed on the CDR3 sequences of TCRs in each sample, so that the total number of CDR3 sequences in each sample is 30,000.

[0071] 2. Randomly select 100 healthy people and 45 non-tumor disease patients from the control group in Example 1.

[0072] 3. According to 100 healthy people, 45 non-tumor disease patients, and 100 colorectal cancer patients from Example 1, and 358 non-colorectal cancer tumor patients and 3 unknown ...

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Abstract

The invention discloses a peripheral blood TCR marker for colorectal cancer as well as a detection kit and application of the peripheral blood TCR marker. The marker comprises at least one of proteinsas shown in a sequence SpecSeq1-100. The sequence is based on a high-throughput sequencing method; only a small amount of peripheral blood needs to be collected; RNA is extracted, a sample is treatedto establish an immune map library, high-throughput sequencing and TCR data analysis are carried out, firstly, a characteristic TCR sequence in colorectal cancer peripheral blood is determined, and then a test result of a sample to be tested is compared with the characteristic TCR sequence so as to determine whether a patient suffers from colorectal cancer or not. The kit can simultaneously compare a huge number of colorectal cancer specific TCR sequences, has higher specificity and accuracy compared with single detection of one or more markers, and improves the diagnosis efficiency.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a peripheral blood TCR marker of colorectal cancer, a detection kit and application thereof. Background technique [0002] Colorectal cancer, also known as colorectal cancer (Carcinoma of Colon and Rectum), is a common malignant tumor in the gastrointestinal tract and the third most common malignant tumor in the world, with an annual incidence of about 1 million and an annual death rate of about 500,000 people. Colorectal cancer is also one of the most common malignant tumors in my country, and its morbidity and mortality are second only to gastric cancer, esophageal cancer and primary liver cancer among digestive system malignant tumors. In my country, the incidence of malignant tumors ranks third (29 / 100,000), and the death rate ranks fifth (14 / 100,000). In recent years, both the incidence and mortality are on the rise. Among the 1.36 million cases of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A61K39/00A61P35/00
CPCG01N33/57446G01N33/57488A61K39/001111A61P35/00G01N2333/7051
Inventor 张志新杨鑫卓越文学平陈思璇
Owner CHENGDU EXAB BIOTECH CO LTD
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