Method for detecting new coronavirus SARS-CoV-2 N protein by adopting nucleic acid aptamer
A nucleic acid aptamer, sars-cov-2 technology, applied in the field of detection of the new coronavirus SARS-CoV-2 N protein, it can not solve the problem, false negative and false positive results are likely to be produced, and clinical laboratories are widely carried out Difficulties, technical and facility requirements, etc., to achieve the effect of strong affinity, easy preparation, low immunogenicity and toxicity
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[0031] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.
[0032] 1. The homology analysis of the N protein of SARS and SARS-CoV-2
[0033] Using bioinformatics methods and bioinformatics comparisons, it was found that the N protein of the 2003 SARS virus and the 2019 new coronavirus SARS-CoV-2 had 91% protein homology. Molecular structure simulation also speculated that the N protein of SARS-CoV-2 has a similar 3D molecular structure to the N protein of SARS. The homology comparison results of the N protein of SARS-CoV and SARS-CoV-2 are as follows figure 1 Shown.
[0034] 2. Design a...
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