Application of solanum lycopersicum SlTRXz gene as restraining target to defence of meloidogyne incognita

A root-knot nematode and gene technology, applied in the fields of application, genetic engineering, and botanical equipment and methods, can solve problems such as affecting plant growth and damage, and achieve the effects of increasing yield, reducing root-knot index and improving resistance.

Inactive Publication Date: 2020-09-29
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Root-knot nematodes mainly infect the fibrous roots and lateral roots of tomatoes. After dissecting the root-knot, it can be observed that there are many small milky-white nematodes buried in the diseased tissue, and the root-knot grows thin new roots, which will be infected again and form Fat, swollen, deformed and tumor-like knots, which cause a series of damage to the root system of tomato, and then affect the growth of the whole plant

Method used

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  • Application of solanum lycopersicum SlTRXz gene as restraining target to defence of meloidogyne incognita
  • Application of solanum lycopersicum SlTRXz gene as restraining target to defence of meloidogyne incognita
  • Application of solanum lycopersicum SlTRXz gene as restraining target to defence of meloidogyne incognita

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Embodiment 1

[0047] Embodiment 1, silencing tomato SlTRXz gene

[0048] 1. Construction of VIGS recombinant vector

[0049] Insert the double-stranded DNA molecule shown in the 109th-407th nucleotides in Sequence 2 of the sequence listing between the XbaI and SmaI restriction sites of the pTRV2 vector to obtain the recombinant plasmid pTRV2-SlTRXz. Recombinant plasmids have been verified by sequencing.

[0050] Insert the double-stranded DNA molecule shown in Sequence 4 of the sequence listing between the XbaI and SmaI restriction sites of the pTRV2 vector to obtain the recombinant plasmid pTRV2-PDS. Recombinant plasmids have been verified by sequencing. The double-stranded DNA molecule shown in Sequence 4 of the sequence listing is a PDS gene fragment and serves as a quality control indicator. PDS is phytoene dehydrogenase, whose expression is inhibited, which will lead to a large accumulation of colorless phytoene, which will lead to a significant decrease in the synthesis of chloroph...

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Abstract

The invention discloses an application of a solanum lycopersicum SlTRXz gene as a restraining target to defence of meloidogyne incognita. The invention claims protection of a method for cultivating plants capable of improving resistance to root-knot nematode. The method comprises the following steps of restraining expression of an SlTRXz gene in plants to obtain the plants capable of improving resistance to root-knot nematode. The content and/or activity of SlTRXz proteins in the plants is reduced, and the resistance of the plants to the root-knot nematode is improved. The expression quantityof the SlTRXz gene in the plants is reduced, and the resistance of the plants to the root-knot nematode is increased. The abundance of the SlTRXz gene in transcription products in the plants is reduced, and the resistance of the plants to the root-knot nematode is improved. A solanum lycopersicum SlTRXz instantaneous silencing strain is obtained through a virus induced gene silencing technique, after the root-knot nematode is inoculated, the inventor finds that the root knot index is significantly, that is to say, the resistance of the plants to the root-knot nematode is improved. Related genes of solanum lycopersicum for adjusting and controlling the resistance to the root-knot nematode are enriched, and reference is provided for solanum lycopersicum breeding and increase of yield in biological adverse circumstances.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of tomato S1TRXz gene as an inhibition target in defense against root-knot nematode incognita. Background technique [0002] Root-knot nematode is a highly destructive plant parasitic nematode. It is one of the most difficult vegetable diseases to control in the world. It is widely distributed all over the world and infects more than 3,000 species of plants. After the disease occurs, it will generally cause a 10% reduction in yield. %-20%, more than 75% in severe cases. [0003] Tomato is one of the most commonly cultivated fruits and vegetables in the world. In my country, it is mainly cultivated on a large scale in greenhouses, plastic greenhouses and other protected facilities. However, in recent years, with the gradual expansion of protected cultivation area and the increase of multiple cropping index, the harm of root-knot nematode has become increasingly serious....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/82
CPCC12N15/8285C12N15/8218C12N9/0051C12Y108/01008
Inventor 赵文超魏婧薇王绍辉黄煌杨瑞高英健
Owner BEIJING UNIV OF AGRI
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