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Novel coronavirus igm/igg antibody joint detection immunochromatographic test strip

An immunochromatography test paper and coronavirus technology, applied in the field of immunochromatography, can solve the problems of long detection time for new coronavirus, inability to carry out large-scale screening, high technical requirements, etc., and achieve short detection time, easy operation, and technical less demanding effect

Active Publication Date: 2021-12-28
NANTONG EGENS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The detection of the new coronavirus 2019-nCoV is mainly PCR-based viral nucleic acid detection, which has the advantages of high specificity and sensitivity. However, this detection method has the following defects: high technical requirements, prone to false negatives, and special specimen requirements Processing requires professional equipment such as PCR amplification instrument and gel electrophoresis, and professional technicians are required to operate and judge the test results. The detection of new coronavirus takes a long time, and large-scale screening cannot be carried out.

Method used

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  • Novel coronavirus igm/igg antibody joint detection immunochromatographic test strip

Examples

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Effect test

Embodiment 1

[0040] A novel coronavirus IgM / IgG antibody joint detection immunochromatographic test strip, such as figure 1 As shown, a sample pad 2, a colloidal gold adsorption pad 3, an antibody carrying membrane 4 and a water-absorbing pad 5 are overlapped successively on the substrate 1;

[0041]Colloidal gold-labeled novel coronavirus recombinant antigen and colloidal gold-labeled mouse IgG are coated on the colloidal gold adsorption pad 3; the sequence of the novel coronavirus recombinant antigen is as shown in SEQ ID NO.1;

[0042] The antibody carrying membrane 4 is provided with a detection line T1, a detection line T2 and a quality control line C at intervals, the detection line is arranged on the side close to the colloidal gold adsorption pad 3, and the quality control line C is arranged on the side near the water absorption pad 5. One side: the detection line T1 is coated with anti-human IgMμ chain monoclonal antibody, the detection line T2 is coated with anti-human IgG antibo...

Embodiment 2

[0045] This embodiment provides a method for preparing the novel coronavirus IgM / IgG antibody joint detection immunochromatography test strip in embodiment 1, comprising the following steps:

[0046] 1. Preparation of antibody-carrying membrane 4:

[0047] (1) Select a nitrocellulose membrane with a pore size of 3 μm to 10 μm, and cut the membrane into specifications with a width of 2.0 cm and a length of 30.5 cm as required, for later use.

[0048] (2) Use 0.1M, pH8.0 Tris-HCL buffer solution to prepare the anti-human IgG monoclonal antibody used for the detection line coating, the antibody concentration is (0.3~0.5) mg / ml, in this embodiment, it is 0.3 mg / ml, anti-human IgM μ chain monoclonal antibody, the antibody concentration is (0.3~0.5) mg / ml, in this embodiment, it is 0.3 mg / ml, and it is prepared for quality control with 0.85% sodium chloride buffer solution with a mass percentage content of 0.85% The anti-mouse IgG polyclonal antibody used for thread coating was ad...

Embodiment 3

[0063] This embodiment provides a novel coronavirus IgM / IgG antibody joint detection immunochromatographic reagent card, including the novel coronavirus IgM / IgG antibody joint detection immunochromatographic test strip in Example 1.

[0064] Further, such as figure 2 As shown, it also includes a card case 7, and the new coronavirus IgM / IgG antibody joint detection immunochromatographic test strip in the embodiment 1 is installed inside the card case 7.

[0065] The clamping case 7 includes an upper cover 8 and a lower cover;

[0066] The upper cover 8 is provided with a sampling hole 9 and a viewing window 10;

[0067] The sample loading hole 9 corresponds to the loading pad 2 of the novel coronavirus IgM / IgG antibody joint detection immunochromatography test strip;

[0068] The window 10 corresponds to the antibody-carrying membrane 4 of the novel coronavirus IgM / IgG antibody joint detection immunochromatography test strip.

[0069] Further, such as image 3 As shown, th...

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Abstract

The present invention relates to the technical field of immunochromatography, in particular to a novel coronavirus IgM / IgG antibody joint detection immunochromatography test strip, comprising: a colloidal gold adsorption pad and an antibody carrying film; the colloidal gold adsorption pad is coated with a colloidal gold marker The new coronavirus recombinant antigen and colloidal gold-labeled mouse IgG; the antibody carrying membrane is provided with a detection line T1, a detection line T2 and a quality control line C at intervals, and the first detection line is coated with an anti-human IgMμ chain Monoclonal antibody, the second detection line is coated with anti-human IgG antibody, and the quality control line is coated with anti-mouse IgG antibody; the above-mentioned immunochromatographic test strip can detect novel coronavirus IgM and IgG antibodies at the same time, and the detection time is as short as only It takes 10 minutes, low cost, easy to operate, low technical requirements, and convenient for on-site sample collection (especially fingertip blood collection), suitable for on-site, instant, and rapid detection.

Description

technical field [0001] The invention relates to the technical field of immunochromatography, in particular to an immunochromatographic test strip, a reagent card, a kit and a joint inspection method for joint detection of novel coronavirus IgM / IgG antibodies. Background technique [0002] On January 12, the World Health Organization (WHO) named the novel coronavirus "2019 novel coronavirus" (2019-nCoV). On February 11, the World Health Organization (WHO) announced that the disease caused by the new coronavirus infection was officially named "COVID-19". On the same day, on February 11, 2020, the International Committee Taxonomy of Viruses (ICTV) announced that the official name of the new coronavirus was severe acute respiratory syndrome coronavirus 2 (severe acute respiratory syndrome coronavirus 2, referred to as SARS-CoV) -2). The virus is a new type of coronavirus strain, which is highly contagious and has a high mortality rate without specific drug treatment. The trans...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577G01N33/569G01N33/558G01N33/531
CPCG01N33/6854G01N33/587G01N33/577G01N33/56983G01N33/558G01N33/531G01N2333/165G01N2469/20
Inventor 欧卫军孙一品张娟顾飞陈兰兰
Owner NANTONG EGENS BIOTECH CO LTD
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