Cardiomyocyte isolation reagent and isolation method

A technology of cardiomyocytes and separation methods, applied in the direction of cell dissociation methods, biochemical equipment and methods, animal cells, etc., can solve the problems of human cardiomyopathy research and experiment limitations, can not fully simulate human physiological and pathological conditions, and achieve Improvement of cell viability and quality, shortening of operation time
CN111748518BActive Publication Date: 2021-12-14深圳百洋智心医学研究有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
深圳百洋智心医学研究有限公司
Publication Date
2021-12-14

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Abstract

The present invention relates to separation reagents and separation methods for mammalian cardiomyocytes, particularly human cardiomyocytes. Specifically, the present invention relates to a method for separating cardiomyocytes, which includes the following steps: a pretreatment step, a cleaning and decalcification step, a digestion step, an equilibration step, and a drip recalcification step, wherein at least An operating fluid contains BLEB and / or PAB or a physiologically acceptable salt thereof. Therefore, the present invention can significantly reduce the operation time of cardiomyocyte separation, and at the same time improve the viability and quality of the separated cells.
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Description

[0001] The scientific research project involved in this application is a medical and health science and technology innovation project of the Chinese Academy of Medical Sciences, and the project number is 2017-I2M-1-003. technical field

[0002] The present invention relates to separation reagents and separation methods for mammalian cardiomyocytes, particularly human cardiomyocytes. Background technique

[0003] In order to study the physiological characteristics of cells and the influence of various factors (including drug action) on cells, so as to meet the needs of scientific research and new drug development, it is often necessary to extract and separate individual cells from living tissues. Generally speaking, cell separation techniques include mechanical dispersion and digestion separation. Among them, the mechanical separation method is suitable for tissues with less fibrous components, such as brain tissue or some embryonic tissues; the digestion and separation metho...

Claims

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