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Large-scale efficient high-yield production and cultivation method for silkworm cordyceps militaris

A cultivation method and technology of silkworm chrysalis, applied in the field of large-scale, high-efficiency and high-yield production and cultivation of silkworm chrysalis Cordyceps militaris, can solve the problems of low efficiency, easy chemical existence, cumbersome operation and other problems of the pupal body disinfection method, and achieve increased added value and economic benefits, and strong packaging , the effect of simple operation

Active Publication Date: 2020-10-13
江西菌草生物科技有限公司
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  • Abstract
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Problems solved by technology

[0006] The present invention aims at the technical defects of the prior art, and provides a large-scale, high-efficiency and high-yield production and cultivation method of silkworm Cordyceps militaris, so as to solve the problems of low efficiency, cumbersome operation, and easy existence of chemical residues in conventional cultivation techniques. technical problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A large-scale, high-efficiency and high-yield production and cultivation method of silkworm Cordyceps militaris, comprising:

[0029] (1) Strain preparation:

[0030] Low-temperature preservation of Cordyceps militaris strains: conventional Cordyceps militaris strains.

[0031] Cordyceps militaris liquid strain: the cultivation steps are as follows:

[0032] (1) Activation of bacteria on test tube slant

[0033] Medium preparation: 200g of potatoes (peeled and boiled juice), 20g of glucose, 5g of peptone, 1g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 20g of agar powder, and 1000ml of water. Dispense into test tubes, sterilize at 121°C for 20 minutes, and place on a slope. Then the preserved Cordyceps militaris strains are inserted into the inclined plane of the test tube under aseptic conditions, and cultivated in the dark at 18-20° C. for 7-10 days.

[0034] (2) Sanjiao liquid seed culture:

[0035] Medium preparation: 200g of potatoes (peeled a...

Embodiment 2

[0049] A large-scale, high-efficiency and high-yield production and cultivation method of silkworm Cordyceps militaris, comprising:

[0050] 1. Disinfection of silkworm chrysalis: Silkworm chrysalis should be used and peeled according to the inoculation amount to ensure the quality of silkworm chrysalis, and the chrysalis is of moderate size and fresh. All dead pupae, diseased pupae, insect pupae, deformed pupae, and soft pupae should all be rejected and not used. Silkworm chrysalis disinfection is, the ozone concentration is 30-40mg / m3, and the treatment time is 10min.

[0051] 2. Inoculation: Under aseptic conditions, use a continuous syringe to inoculate 0.2-0.3ml of liquid bacteria into each silkworm chrysalis.

[0052]3. Bacteria cultivation: the inoculated silkworm chrysalis are placed in the culture tray, which can be stacked, and the number of stacks in a single group does not exceed four layers. The bacteria culture tray does not need to be covered, it can be direct...

Embodiment 3

[0061] A large-scale high-efficiency and high-yield production and cultivation method of silkworm Cordyceps militaris, comprising the following steps:

[0062] 1) Take live silkworm chrysalis and place them at 30-40 mg / m 3 In the ozone environment, keep it for 10 minutes;

[0063] 2) Take the silkworm chrysalis treated in step 1), and inject 0.2-0.3 mL of liquid bacteria into each silkworm chrysalis under aseptic conditions;

[0064] 3) placing the silkworm chrysalis inoculated in step 2) in a disc-shaped container, and cultured for 5 days at an ambient temperature of 15-17° C. and a relative air humidity of 50%-70% RH in the dark without covering the upper end of the container;

[0065] 4) Adjust the culture temperature to 20-22°C and the relative air humidity above 80% RH, continue the culture, ventilate every day, and light for 10-12 hours a day;

[0066] 5) Harvest when the fruiting bodies reach 5-7cm in length;

[0067] 6) 55 ~ 60 ℃ hot air drying.

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Abstract

The invention provides a large-scale efficient high-yield production and cultivation method for silkworm cordyceps militaris. According to the technical scheme, silkworm chrysalis disinfection is improved into ozone disinfection, and centralized open type fungus cultivation is adopted after inoculation. Specifically, silkworm chrysalis is placed in an ozone environment with the concentration of 30-40 mg / m<3>, and disinfection is conducted for 10 min; after inoculation, a disc-shaped container is used for centralized open type fungus cultivation, the environment temperature is 15-17 DEG C, therelative air humidity is 50%-60% RH, light-proof cultivation is conducted, and the period is 5d. Ozone disinfection is adopted, disinfection dead corners do not exist, a large number of silkworm chrysalis can be treated at a time, and operation is easy; no reagent needs to be prepared, and the problems of chemical residues and environmental pollution are avoided. Meanwhile, centralized managementopen type fungus cultivation is adopted, hyphae can obtain sufficient oxygen in the growth process and dissipate heat generated during breathing in time, the rigidity time can be effectively shortened, and the rigidity rate is increased. Compared with the prior art, the method can reduce the cost investment by 80%.

Description

technical field [0001] The invention relates to the technical field of Cordyceps militaris cultivation, in particular to a large-scale, high-efficiency and high-yield production and cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris. The scientific name is Cordyceps militaris (L.) link. The genus Ascomycota, Sclerotinia, Spheroides, Ergotaceae, Cordyceps. It is a rare edible and medicinal fungus. Modern pharmaceutical research shows that Cordyceps militaris contains nucleosides, polysaccharides, cordycepin, sterols and other components that are the same as Cordyceps sinensis. It has therapeutic effects on diseases such as the immune system, nervous system and cardiovascular system, and has anti-tumor and anti-cancer effects, and has broad application prospects. At present, the cultivation of Cordyceps militaris mostly uses grains such as wheat and rice as the cultivation sub...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A01G18/50
CPCA01G18/00A01G18/20A01G18/50
Inventor 姚丽华陈帅刘新平常军
Owner 江西菌草生物科技有限公司
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