Regeneration and propagation method for optimized fresh ginger virus-free seedlings and development of industrialized flow of optimized fresh ginger virus-free seedlings

A technology for detoxifying seedlings and ginger, applied in the field of plant tissue culture, can solve the problems of easy-to-proliferate bacteria, low reproduction efficiency, poor growth after multiple subcultures, etc., and achieves the effects of short cycle, high reproduction efficiency and good state.

Pending Publication Date: 2020-10-16
潍坊峡兴农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing ginger tissue culture technology still has the disadvantages of easy bacteria growth, low reproduction efficiency, and poor growth after multiple subcultures, so it cannot be applied in a large area.

Method used

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  • Regeneration and propagation method for optimized fresh ginger virus-free seedlings and development of industrialized flow of optimized fresh ginger virus-free seedlings
  • Regeneration and propagation method for optimized fresh ginger virus-free seedlings and development of industrialized flow of optimized fresh ginger virus-free seedlings
  • Regeneration and propagation method for optimized fresh ginger virus-free seedlings and development of industrialized flow of optimized fresh ginger virus-free seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The selection of embodiment 1, ginger explant

[0030] Select ginger pieces that are fat, plump, bright in skin color, fresh in flesh, not dry or shrink, not rotten, not frozen, and hard in texture, and accelerate germination at 28°C and 45% humidity for about 30 days, until the ginger buds grow to 1 ~2cm shoots are cut off for later use.

[0031] The shape of ginger explants directly affects the germination rate and growth strength. The traditional method does not strictly select the explants, which will cause the germination rate and growth of offspring ginger seedlings to be uneven. In this study, the selection of ginger explants was explored. The specific method is as follows:

[0032] (1) Select the ginger buds cultivated above, process with 75% alcohol for 1min, 0.1% mercuric chloride for 10min, wash with sterile water for 3-5 times, peel off the leaf primordia, and obtain 20 ginger bud explants in different states (such as figure 1 shown);

[0033] (2) Place...

Embodiment 2

[0037] Embodiment 2, ginger explant disinfection condition optimization

[0038] Ginger explants will carry a variety of bacteria and fungi, and incomplete disinfection will reduce the overall germination rate of ginger explants and affect the growth of offspring. In the traditional method, the use of less disinfectant dose will cause high pollution rate, and high disinfectant dose will cause low germination rate. In this study, different disinfectant combinations were used, different disinfection conditions were optimized, and a disinfection method with low contamination rate and high germination rate was identified.

[0039] (1) Clean the ginger buds with tap water after peeling them off, then remove the outermost skin, and carry out disinfection treatment in the ultra-clean workbench;

[0040] (2) Different disinfection conditions are as follows:

[0041] Disinfection method 1: 75% alcohol treatment for 1 minute, 0.1% mercury chloride treatment for 10 minutes, and sterile...

Embodiment 3

[0049] Embodiment 3, the selection of induction medium hormone kind

[0050] The germination rate of ginger explants and the differentiation rate of offspring are affected by the types and concentrations of hormones. In this study, the types of cytokinins were screened.

[0051] (1) Cleaning with tap water after peeling off the ginger buds, then removing the outermost epidermis, carrying out disinfection treatment (disinfection method 2) in the ultra-clean workbench, and then peeling off the leaf primordia to obtain the C state explants (see figure 1 ) 60 in total, divided into 3 groups on average;

[0052] (2) Prepare the induction medium of the following different cytokinins and carry out the induction treatment on the explants;

[0053] Medium 1: MS medium + 2mg / LTDZ + 0.5mg / LNAA + 8g agar;

[0054] Medium 2: MS medium + 2mg / LKT + 0.5mg / LNAA + 8g agar;

[0055] Medium three: MS medium + 2mg / L6-BA + 0.5mg / LNAA + 8g agar;

[0056] (3) The culture condition was 26°C and li...

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Abstract

The invention discloses a regeneration and propagation method for optimized fresh ginger virus-free seedlings and development of the industrialized flow of the optimized fresh ginger virus-free seedlings. The regeneration and propagation method is characterized in that an optimal explant type, disinfection method, cytokinin, and induction medium formula, seedling hardening condition and cultivation condition are determined. It requires 3.5-4.5 months for the optimized fresh ginger virus-free seedlings from explants to TC0 rooting seedlings, and it takes about 1.5 months for each time of subsequent subculture; the bacteria pollution rate in the whole process is less than 5%; the budding percentage of TC0 can reach up to 800%; the differentiation efficiency can reach up to 300% for each subsequent subculture; the rooting rate can reach 100%; the survival rate of transplanting of tissue cultured seedlings can reach 80% or more, and the rooting seedlings can be applied to industrializationas a ginger seed after being propagated in a field for 2-3 generations; and the virus-free seedlings in the method have high propagation efficiency, a good condition and a short cycle.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for multiplying virus-free ginger seedlings and its industrialization process. Background technique [0002] Ginger is the rhizome of Zingiberaceae. It is a perennial herb. It should grow in a mild, shady and humid environment. Due to different varieties, it has various colors such as white, beige, brown, yellow and golden yellow. Xin, has high nutritional value and medicinal value. Ginger has a long history as a food and medicinal material. Traditional Chinese medicine believes that ginger is a good product in medicine. It has the effects of relieving exterior cold, anti-inflammatory and antibacterial, and can be used to treat symptoms such as colds, colds, and vomiting. Aging and anti-tumor have a certain effect, and can inhibit the growth of cancer cells to a certain extent; in addition, ginger has the effect of dispelling cold, promoting blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 公小君刘凤丽林爱华王喜萍
Owner 潍坊峡兴农业科技有限公司
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