Detection kit for individualized medication-related gene polymorphism and application thereof
A gene polymorphism and detection kit technology, applied in the field of genetic diagnosis, can solve the problems of time-consuming and laborious, expensive equipment and consumables, and inability to characterize
Pending Publication Date: 2020-10-20
广西医大睿谷医学检验有限公司
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Problems solved by technology
These traditional methods are not qualitative, or time-consuming and labor-intensive, requiring expensive equipment and consumables. More importantly, these methods are difficult to detect multiple mutation sites of different genes at the same time
So far, there is no kit for rapid detection of genes for individualized antihypertensive drugs on the market. In summary, the development of a fast, efficient and accurate genetic detection kit for guiding the safe use of antihypertensive drugs has great significance. clinical and social significance
Method used
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Embodiment 1
[0052] 1. DNA extraction from samples to be tested
[0053] One peripheral venous blood sample (EDTA anticoagulant) was collected, and 200 μl of blood was taken from the sample, which was extracted with Tiangen Blood Genomic DNA Extraction Kit (spin column type). For details, please refer to the kit instruction manual.
[0054] 2. Gene Amplification Reagent Preparation
[0055] Take out 4 tubes of PCR mixture solution from the kit and thaw at room temperature in the dark, vortex and mix well, and then centrifuge at 8000rpm for 10s. Take out the DNA polymerase from the kit and centrifuge at 8000rpm for 10s. Add 0.5 μl of DNA polymerase to 36.5 μl of PCR mixture per reaction and mix. Each PCR mixture is prepared for 3 reactions, and a total of 12 reaction solutions are prepared. Mix the prepared amplification reagents thoroughly and centrifuge at 8000rpm for 10s , add 37 μl of the prepared amplification reagents to the 12 set PCR reaction tubes, mark them and transfer them to ...
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The invention relates to a specific primer probe combination for guiding individual medication related gene SNP. The primer probe combination comprises a specific primer probe combination aiming at SNP of rs16960228, rs1801253, rs12750834 and rs776746 genes. The invention further provides a kit comprising the primer combination and application of the kit. The combination has the beneficial effectsthat: the invention provides a method for simultaneously detecting the polymorphism of four genes: rs16960228, rs1801253, rs12750834 and rs776746 and accurately distinguishing heterozygotes and homozygotes, and provides a typing kit for simultaneously guiding individualized medication related gene SNP of metoprolol, hydrochlorothiazide, valsartan and amlodipine and application. The kit is short in detection time, simple and convenient to operate, accurate and reliable in result and suitable for clinical popularization and application.
Description
technical field [0001] The invention relates to the technical field of gene diagnosis, in particular to a combination of primers and probes for the detection of gene polymorphisms for individualized medication guidance of antihypertensive drugs, a kit and applications thereof. Background technique [0002] As the most important risk factor for cardiovascular and cerebrovascular diseases, high blood pressure has a serious epidemic situation. Its main complications, such as stroke, myocardial infarction, heart failure and chronic kidney disease, have a high disability and mortality rate, which seriously consumes medical and social resources and brings great harm to families. It has become an important public health problem in my country and has caused a heavy burden on society and society. [0003] Genetic background is an important influencing factor for the large variability in drug response among different individuals. Drugs enter the human body and play a role through the ...
Claims
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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/106C12Q2600/156C12Q2600/16C12Q2600/172C12Q2531/113C12Q2537/143C12Q2561/101C12Q2563/107
Inventor 廖南清梁浩袁志兵张林峰
Owner 广西医大睿谷医学检验有限公司