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Whey protein isolate nanofiber/carbon nanotube composite material and preparation method thereof

A whey protein isolate and carbon nanotube technology, which is applied in the field of whey protein isolate nanofiber/carbon nanotube composite materials and their preparation, and can solve the problems of in vivo research or in vitro research that have not been reported.

Active Publication Date: 2020-10-27
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, neither in vivo nor in vitro studies of carbon nanomaterials in the study of loading metformin hydrochloride and cutting fibers have been reported.

Method used

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  • Whey protein isolate nanofiber/carbon nanotube composite material and preparation method thereof
  • Whey protein isolate nanofiber/carbon nanotube composite material and preparation method thereof
  • Whey protein isolate nanofiber/carbon nanotube composite material and preparation method thereof

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Embodiment 1

[0030] A preparation method of whey protein isolate nanofiber / carbon nanotube composite material, comprising the following steps:

[0031] (1) Preparation of whey protein isolate nanofiber solution:

[0032] Stir and dissolve whey protein isolate with a protein content of 97.80% and no lecithin in deionized water to prepare a stock solution with a mass concentration of 6 wt%;

[0033] Add 1M hydrochloric acid solution to adjust the pH value of the stock solution to 4.75;

[0034] Then the stock solution with a pH value of 4.75 was put into a centrifuge at 10000rpm and centrifuged for 60min;

[0035] Filter the supernatant with a supply syringe filter with a pore size of 0.45 μm;

[0036] The pH value of the filtered supernatant was adjusted to 2 with 6M hydrochloric acid solution;

[0037] Determine the protein concentration of the supernatant using a UV spectrophotometer and a calibration curve of known whey protein isolate concentrations at a wavelength of 278 nm;

[003...

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Abstract

The invention relates to a whey protein isolate nanofiber / carbon nanotube composite material and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a whey protein isolate nanofiber solution: dissolving a whey protein isolate solution into deionized water through stirring, so as to prepare a stock solution with a mass concentration of 6wt%; adding a hydrochloric acid solution, so as to regulate the pH value of the stock solution to be 4.75; carrying out centrifugal treatment; filtering supernate; regulating the pH value of the supernate to be 2 byvirtue of the hydrochloric acid solution; diluting the supernate by virtue of the hydrochloric acid solution until the protein concentration is 2wt%; carrying out water-bath heating; and carrying outstirring at a speed of 290rpm; and (2) carrying out ultrasonic treatment on a carbon nanotube solution with a mass concentration of 0.05wt%-0.15wt%; adding the whey protein isolate nanofiber solutionwith the mass concentration of 1%, and carrying out magnetic stirring; carrying out hydrothermal reaction; putting the mixed solution into a drying tank for drying; and carrying out grinding so as toobtain powder. The whey protein isolate nanofiber / carbon nanotube composite material is capable of bearing metformin hydrochloride and cutting whey protein isolate nanofibers. The whey protein isolatenanofiber / carbon nanotube composite material belongs to the technical field of carbon nanocomposite materials.

Description

technical field [0001] The invention belongs to the technical field of carbon nanocomposite materials, and in particular relates to a whey protein isolate nanofiber / carbon nanotube composite material and a preparation method thereof. Background technique [0002] Whey protein, known as the king of protein, is a protein extracted from milk. It has the characteristics of high nutritional value, easy digestion and absorption, and contains a variety of active ingredients. It is recognized as one of the high-quality protein supplements for the human body. With the With the continuous development of product technology and the increasingly fierce competition of protein products, whey protein products are also gradually diversifying. Common whey protein materials are whey protein isolate and so on. When Wang used whey protein concentrate to prepare fiber solution, he found that under the heating condition of 0-5h (pH1.8), the nanofiber solution gradually changed from transparent lig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/04A61K31/155A61P3/10B82Y40/00B82Y5/00D01F4/00
CPCA61K47/42A61K47/02A61K31/155A61P3/10B82Y40/00B82Y5/00D01F4/00
Inventor 康宁高利珍
Owner TAIYUAN UNIV OF TECH
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