Method for degrading triphenyl phosphate by using pycnoporus sanguineus, and application thereof
A technology of Microporosa haemorrhoids and triphenyl phosphate, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of poor environmental adaptability, low efficiency, weak resistance, etc. Achieve strong environmental adaptability, fast and efficient degradation, and good degradation effect
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Embodiment 1
[0033] Example 1 Degradation analysis of Miporosaus sanguineus to triphenyl phosphate
[0034] (1) Transfer the blood-red dense pore strains stored on the slant at 4°C to the PDA plate medium by streaking, and place it in a constant temperature incubator at 37°C for static culture for 5 days (days), until the surface of the plate grows A large number of white powdery spore layers were produced, and the plate was washed with sterile physiological saline, and the spores on the surface of the plate were gently scraped off with a sterile inoculation loop, and filtered through double-layer sterile gauze to obtain the spore liquid of Microporia haemorrhoids. Use a spectrophotometer to measure the absorbance value of the spore liquid at 600nm, and dilute the spore liquid with sterile saline to adjust the absorbance value to OD 600 =0.85, the concentration of the spore liquid is about 2.0×10 6 CFU / mL; where:
[0035] The composition of the PDA plate medium is: glucose 20g, KH 2 PO ...
Embodiment 2
[0044] The method is the same as in Example 1, the difference is that in the degradation process of step (2), the concentration of triphenyl phosphate in the liquid medium is 10 mg / L, the pH of the liquid medium is 6, and the culture conditions are: the temperature is 30 ° C, the shaker The rotating speed is 160rpm, and the incubation time is 5d. Finally, the degradation rate of triphenyl phosphate was 62.3%.
Embodiment 3
[0046] The method is the same as in Example 1, the difference is that in the degradation process of step (2), the concentration of triphenyl phosphate in the liquid medium is 10 mg / L, the pH of the liquid medium is 4, and the culture conditions are: the temperature is 30 ° C, the shaker The rotating speed is 160rpm, and the incubation time is 5d. Finally, the degradation rate of triphenyl phosphate by Microporus haemorrhoids was 60.8%.
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