Liquid composition containing high concentration of DNA fragment mixture and having fluidity and preparation method therefor

A composition and fluidity technology, applied in the field of liquid composition and preparation thereof, can solve the problems of undisclosed DNA fragment mixture gelation, undisclosed, etc., achieve high stability, no change in physical properties, and continuously maintain fluidity Effect

Active Publication Date: 2020-10-27
RES MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] As a prior art, Korean Registered Patent No. 1459185 discloses a nucleic acid delivery complex in which nucleic acid is bound to a spermine copolymer, but does not disclose or suggest at all the effect of dissolving a high-concentration DNA fragment mixture using the cationic additive of the present invention
In addition, Korean Laid-Open Patent No. 2011-0129969 discloses a preparation containing polyamine derivatives and nucleic acids, but does not disclose or suggest the effect of dissolving the high-concentration fragment mixture of the present invention at all
[0010] As a non-patent literature, Sung, B.K. (2012) discloses DNA condensation using spermine, but does not disclose the gelation of the high-concentration DNA fragment mixture and the effect of preventing gelation to dissolve the high-concentration DNA fragment mixture of the present invention

Method used

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  • Liquid composition containing high concentration of DNA fragment mixture and having fluidity and preparation method therefor
  • Liquid composition containing high concentration of DNA fragment mixture and having fluidity and preparation method therefor
  • Liquid composition containing high concentration of DNA fragment mixture and having fluidity and preparation method therefor

Examples

Experimental program
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Effect test

Embodiment 1

[0065]

[0066] The DNA fragment mixture of the present invention has utilized polydeoxynucleotide (product name: Pharmaceutical Research Products Co., Ltd., Korea), and the cationic additive utilizes spermine (spermine).

[0067] As a mixture of DNA fragments, the Add to 0.4% sodium chloride (sodium chloride, NaCl) buffer solution, and use a hot stirrer to dissolve at 80 ° C for 20 minutes to prepare Stock solution.

[0068] As a cationic additive, spermine was added into a 0.4% sodium chloride (sodium chloride, NaCl) buffer solution and dissolved at room temperature for more than one hour to prepare a spermine storage solution.

[0069] prepared above The stock solution and the spermine stock solution were mixed so as to become the concentration conditions shown in Table 1 below to prepare the compositions of Examples 1-1 to 1-12. At this point, stir at 80°C While storing the solution, slowly add the spermine storage solution and stir for more than 1 hour and mi...

Embodiment 2

[0077]

[0078] The stability and physical properties of a liquid composition greatly affect the development of formulations using it and the treatment method depending on the treatment site. Therefore, by analyzing whether the physical properties of the fluid liquid composition containing a high-concentration DNA fragment mixture of the present invention change according to external force or temperature, it can be confirmed whether the fluidity is stably maintained.

Embodiment 2-1

[0079] Example 2-1. Confirmation of physical properties using a rheometer (rheometer)

[0080] Viscosity is related to a fluid's internal resistance to motion, or fluidity, and is affected by temperature. Elasticity is the property of a substance deformed by an external force to return to its original shape when the force is removed from it. Liquids such as water do not deform elastically. Therefore, the absolute value and relative ratio of the viscosity and elasticity of a substance are important material functions that determine its response to external forces.

[0081] Thus, fluidity and stability were confirmed by analyzing the viscosity and elasticity changes of the fluidic liquid composition containing a high-concentration DNA fragment mixture of the present invention.

[0082] Using the liquid composition and water prepared in said Example 1 and Comparative Example 1 as a control, a rheometer is used to measure the storage modulus (storage modulus, G') according to t...

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Abstract

The present invention relates to a liquid composition containing a high concentration of a DNA fragment mixture and a preparation method therefor. A liquid composition containing a high concentrationof a DNA fragment mixture and having fluidity was prepared. The liquid composition that contains a high concentration of a DNA fragment mixture and has fluidity was identified to maintain a pharmaceutical activity, have high stability even during long-term storage, and be effectively injectable to the human body, whereby a high concentration of a DNA fragment mixture having fluidity is expected tobe applicable to the development of various formulations such as injections, liquid agents, creams, and the like.

Description

technical field [0001] The present invention relates to a liquid composition containing a DNA fragment mixture at a high concentration and having fluidity and a preparation method thereof. The liquid composition contained in the state. Background technique [0002] Nucleic acid (nucleic acid) is the DNA and RNA present in the nucleus, which is composed of purine bases (adenine, A; guanine, G) or pyrimidine bases (cytosine, C; thymine, T; Uracil, U), a five-carbon sugar, and a polymer of nucleotides composed of phosphoric acid. This type of nucleic acid has been considered as a substance for storing and transmitting genetic information, but recently, it has been known that nucleic acid is broken down into small pieces outside the cell and exerts selective binding to receptors on the cell surface to be activated into the cell The role of signaling and the movement of small pieces of nucleic acid into the cell induces DNA synthesis rapidly with very little energy, so new func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K47/18A61K47/34A61K47/42A61K47/36A61K48/00
CPCA61K47/42A61K47/34A61K47/36A61K47/18A61K9/08A61K31/711A61K35/60A61K45/06A61K2300/00A61K47/183A61K48/00A61K31/713
Inventor 金益洙李修渊
Owner RES MEDICAL
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