Dendrobium officinale seedling culture medium and seedling culture method

A technology of dendrobium candidum and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high production cost, restriction of dendrobium candidum and high technical requirements in tissue culture factories, so as to save the transfer link, Weak adhesiveness, the effect of solving a lot of pollution

Inactive Publication Date: 2020-10-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the industrial seedling cultivation system of Dendrobium candidum has been established, the high production cost and high technical requirements of tissue culture factories are still a major problem restricting the development of Dendrobium candidum industry

Method used

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  • Dendrobium officinale seedling culture medium and seedling culture method
  • Dendrobium officinale seedling culture medium and seedling culture method
  • Dendrobium officinale seedling culture medium and seedling culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Preparation of liquid medium, the formula of the liquid medium is: MS+0.3mg L -1 NAA+1.0mg·L -1 6-BA+5% Banana Juice+10% Potato Juice+5% Coconut Juice+30g·L -1 Add 100 g of dried coconut bran powder to every liter of prepared liquid culture medium with sucrose, and the pH is 5.8 to 5.9 to obtain a seedling culture medium for Dendrobium candidum.

Embodiment 2

[0042] Preparation of liquid medium, the formula of the liquid medium is: MS+0.4mg L -1 NAA+1.1mg·L -1 6-BA+6% Banana Juice+9% Potato Juice+4% Coconut Juice+29g·L -1 Add 90 g of dried coconut bran powder to every liter of prepared liquid culture medium, the pH is 5.8 to 5.9 to obtain the Dendrobium officinale seedling culture medium.

Embodiment 3

[0044] Preparation of liquid medium, the formula of the liquid medium is: MS+0.2mg L -1 NAA+1.2mg·L -1 6-BA+4% Banana Juice+11% Potato Juice+6% Coconut Juice+31g·L -1 Add 110 g of dried coconut bran powder to every liter of prepared liquid culture medium to obtain sucrose, and the pH is 5.8 to 5.9 to obtain a culture medium for dendrobium candidum.

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Abstract

The invention discloses a dendrobium officinale seedling culture medium and a seedling culture method, and relates to the technical field of traditional Chinese medicine seedling culture. The dendrobium officinale seedling culture medium is prepared from a liquid medium and coco coir by mixing. A dendrobium officinale seed suspension is seeded on the medium by spot sowing to carry out dendrobium officinale seedling culture. The coco coir has the characteristics of water absorption, air ventilation and sun shading, besides, the price is low, the production cost is saved, and obtained dendrobiumofficinale seedlings have high adaptability and high transplanting survival rate. The dendrobium officinale seeds are scattered into a nutrient solution, the seed suspension is prepared, the transferproblem caused by insufficient growing space of test-tube plantlets in the medium is solved by controlling density, the transfer link in a seedling production process is omitted, and labor cost is greatly saved; and meanwhile, the problem of a large amount of pollution of inoculated tissue culture seedlings due to pollution of the suspension in a previous production process is also solved.

Description

technical field [0001] The invention belongs to the technical field of raising seedlings of traditional Chinese medicinal materials, and relates to a seedling raising medium and a method for raising dendrobium officinale. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae. The traditional precious and bulk Chinese medicinal materials have a long history of application and have been known as "the gold in medicine" since ancient times. Modern pharmacological studies have shown that Dendrobium officinale polysaccharides, stilbenes, flavonoids, alkaloids and volatile components are its main medicinal substances, which have the functions of enhancing immunity, anti-tumor, anti-oxidation, anti-bacterial, anti-inflammatory, lowering blood sugar, and protecting the liver. Stomach and other functions. [0003] Dendrobium candidum has very small seeds, incomplete embryonic development, and no endosperm...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 向增旭王将罗丽娜张子璇
Owner NANJING AGRICULTURAL UNIVERSITY
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