Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cervus elaphus linnaeus soluble protein extract and extraction method and application thereof

A protein extract and extraction method technology, applied in the field of red deer antler soluble protein extract and its extraction, can solve the problems of protein extraction rate and the lack of activity of the extract, etc.

Active Publication Date: 2020-10-30
CHANGCHUN UNIV OF CHINESE MEDICINE
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the most widely used protein extraction processes mainly include hot water extraction, alkaline method, ultrasonic method, microwave method, etc. However, these methods are lacking in protein extraction rate and activity of the extract.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cervus elaphus linnaeus soluble protein extract and extraction method and application thereof
  • Cervus elaphus linnaeus soluble protein extract and extraction method and application thereof
  • Cervus elaphus linnaeus soluble protein extract and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The selection of colloidal grinding tooth gap (raw material slurry crushing degree)

[0026] Take 10g of deer antler fine powder as a group, a total of 7 groups, add 10 times the volume of water and mix well, then colloid mill homogenate for 30 minutes, adjust the colloid mill tooth gap to 10, 30, 50, 70, 90, 110, 130 μm , 4000r / min centrifugation for 10min to obtain the extract, and determine the protein extraction rate. Each experiment was repeated three times and the average value was calculated.

[0027] Calculation method of velvet antler protein extraction rate: refer to Bradford method, accurately measure 1mg / ml bovine serum albumin (BSA) standard protein solution 0, 10, 20, 40, 60, 80, 100μL, distilled water to 100μL, shake Evenly, prepare BSA reference substance solutions with mass concentrations of 0.1, 0.2, 0.4, 0.6, 0.8, and 1.0 mg / ml, respectively. 5 mL of Coomassie Brilliant Blue G-250 staining solution was added for staining, and after reac...

Embodiment 2

[0029] The selection of embodiment 2 colloid mill grinding time

[0030] Take 10g of deer antler fine powder as a group, a total of 7 groups, add 10 times the volume of water and mix well, adjust the gap between the teeth of the colloid mill to 50μm, and the homogenization time of the colloid mill is 10, 15, 20, 25, 30, 35 , 40min later, centrifuge at 4000r / min for 10min to obtain the extract, and measure the protein extraction rate. The calculation method of velvet protein extraction rate is the same as Example 1. Each experiment was repeated three times and the average value was calculated.

[0031] The gap between the colloid grinding teeth is 50 μm, and the results of investigating the effect of different grinding times on the extraction rate of velvet antler protein are as follows: figure 2 shown. Depend on figure 2 It can be seen that when the grinding time is 30 min, the protein extraction rate is the highest, and when the time continues to prolong, the increase o...

Embodiment 3

[0032] Example 3 Enzymatic extraction of velvet protein technology single factor test

[0033] Take 10ml of extract solution from each group, adjust the pH of the solution to 6.5, add a certain amount or different amount of papain, bathe in water for a certain or different time at different temperatures or at the optimum temperature of the enzyme at 55°C, centrifuge at 4000r / min for 10 minutes to obtain enzymatic extraction solution, the protein content was determined and the protein extraction rate was calculated. The calculation method of velvet protein extraction rate is the same as Example 1. Each time a single-factor variable test is done, the best parameters obtained from the previous single-factor verification are used as the quantitative parameters of the next single-factor test, and the different levels of each factor in the orthogonal test are determined.

[0034] Take 10ml extract from each group, adjust the pH to 6.5, add papain 10, 20, 30, 40, 50, 60mg / g respecti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cervus elaphus linnaeus soluble protein extract and an extraction method and application thereof. Soluble protein in pilose antler is extracted by adopting a colloid mill grinding combined with an enzyme method; the cornua cervi pantotrichum protein extraction rate is used as an evaluation index to investigate the influence of enzymolysis time, temperature, enzyme addition amount and the like on a protein extraction process, the optimal process for extracting the cervus elaphus linnaeus soluble protein by the colloid mill assisted enzyme is obtained, and the non-specific immunocompetence of the protein extract is further studied; the phagocytic index of the mononuclear macrophage is determined through a carbon clearance test; indexes of serum lysozyme, acid phosphatase and the like show that the pilose antler soluble protein has an obvious immunoregulation effect and good immunocompetence, and provides a scientific basis for deep research and development of the precious medicinal material pilose antler in the aspect of preparation of medicines for treating low immunocompetence caused by cyclophosphamide.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a soluble protein extract of red deer antler and its extraction method and application. Background technique [0002] Antler is the unossified and densely hairy young horns of the male deer (Cervus nippon Temminck) or red deer (Cervus elaphus Temminck) of the deer family. It is a precious Chinese medicinal material. Antler contains phospholipids, glycolipids, gelatin, hormones, fatty acids, amino acids, proteins, calcium, phosphorus, magnesium, sodium and other ingredients, of which amino acids account for more than half of the total ingredients. Ancient doctors believed that the essence of deer lies in the horns, and the velvet is the bud of the horns, the gas is all but not vented, so the power of nourishing yang and blood is the strongest. Li Shizhen of the Ming Dynasty said in the "Compendium of Materia Medica" that velvet antler "is good at nourishing the kidney ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/01A61P37/04
CPCC12P21/06A61K38/015A61P37/04
Inventor 高文义贡济宇蔡广知陶雪慧王康宇李妍黄琼陶贵斌
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com