A preparation method of nanometer drug-loaded micelles loaded with daptomycin and small-molecule hydrophobic drugs
A hydrophobic drug, daptomycin technology, applied in the field of biomedical nanomaterials, can solve the problems of difficult large-scale preparation and accurate preparation of carriers, complex synthesis process, complex structure, etc., and achieve excellent resistance to non-specific protein adsorption Performance, simple preparation conditions, and mild reaction conditions
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Embodiment 1
[0041] Doxorubicin is selected as the small molecule hydrophobic drug of this embodiment, and the method for preparing nano-drug-loaded micelles includes the following steps:
[0042] S1, prepare doxorubicin hydrochloride methanol solution;
[0043] Dissolve 1.5 mg of doxorubicin hydrochloride in 1 mL of methanol to prepare 1.5 mg / mL of doxorubicin hydrochloride methanol solution;
[0044] Preparation of doxorubicin methanol solution;
[0045] Take 400 μL of doxorubicin hydrochloride methanol solution, then add 16.2 μL of triethylamine, and sonicate for 1 h in the dark to obtain doxorubicin methanol solution;
[0046] S2, prepare daptomycin methanol solution;
[0047] According to the mass ratio of methanol to daptomycin 1:0.0076, prepare 6 mg / mL daptomycin methanol solution;
[0048] S3, preparing nano-drug loaded micelles;
[0049] Take 300 μL of doxorubicin methanol solution and 750 μL of daptomycin methanol solution in a 5 mL round-bottomed flask, and at room temperatu...
Embodiment 2
[0060] S1, prepare doxorubicin hydrochloride methanol solution;
[0061] Dissolve 1 mg doxorubicin hydrochloride in 1 mL methanol to prepare 1 mg / mL doxorubicin hydrochloride methanol solution;
[0062] Preparation of doxorubicin methanol solution;
[0063] Take 400 μL of doxorubicin hydrochloride methanol solution, then add 0.3 μL of triethylamine, and sonicate in the dark for 1 h to obtain doxorubicin methanol solution;
[0064] S2, prepare daptomycin methanol solution;
[0065] According to the mass ratio of methanol to daptomycin 1:0.001, prepare 1 mg / mL daptomycin methanol solution;
[0066] S3, preparing nano-drug loaded micelles;
[0067] Take 300 μL of doxorubicin methanol solution and 300 μL of daptomycin methanol solution in a 5 mL round-bottomed flask, at room temperature, in a water bath at a speed of 3000 rpm for 6 h in the dark and in a temperature bath, and then the mixed methanol solution was added to the flask. It was added dropwise to 12 mL of water at a ...
Embodiment 3
[0069] S1, prepare doxorubicin hydrochloride methanol solution;
[0070] Dissolve 10 mg of doxorubicin hydrochloride in 1 mL of methanol to prepare a 10 mg / mL doxorubicin methanol solution;
[0071] Preparation of doxorubicin methanol solution;
[0072] Take 200 μL of doxorubicin hydrochloride methanol solution, then add 32.4 μL of triethylamine, and sonicate for 1 h in the dark to obtain doxorubicin methanol solution;
[0073] S2, prepare daptomycin methanol solution;
[0074] According to the mass ratio of methanol to daptomycin 1:0.0126, prepare 10 mg / mL daptomycin methanol solution;
[0075] S3, preparing nano-drug loaded micelles;
[0076] Take 100 μL of doxorubicin methanol solution and 10 mL of daptomycin methanol solution in a 25 mL round-bottomed flask, at room temperature, heat in a water bath at a speed of 100 rpm for 6 h in the dark and in a warm bath, and then the mixed methanol solution is gradually added. It was added dropwise to 8 mL of water at a rate of 0...
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