Method for constructing lung cancer in-situ PDX model through percutaneous puncture inoculation

Abstract

The invention discloses a method for constructing a lung cancer in-situ PDX model through percutaneous puncture inoculation. The method comprises the following steps of (1) tumor sample collection; (2) tumor sample treatment; (3) mouse inoculation; (4) feeding and observation; (5) PET-CT inspection; and (6) pathological detection. According to the method, the method comprising the steps of preparation of a lung in-situ tissue inoculation sample of a percutaneous lung puncture tissue block, lung in-situ inoculation of an immunodeficient mouse and conventional feeding and observation of the immunodeficient mouse is adopted so that a human lung cancer tissue block can be inoculated into a lung tissue of the immunodeficient mouse so as to construct the human lung cancer in-situ PDX model, anda percutaneous puncture point, the angle of a puncture needle and the needle insertion depth are further optimized, so that the success rate of mouse inoculation is greatly improved; and the method fully reserves a microenvironment of the tumor tissue of a patient, further highly simulate the biological mode of human tumor, can better reproduce the pathological and physiological characteristics ofthe lung cancer patient, and has important scientific research, and medical value and economic value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing an orthotopic PDX model of lung cancer inoculated by percutaneous puncture. Background technique [0002] Human-derived tumor xenografting model (Patient-derived xenografting, PDX) refers to the in vivo animal model constructed by inoculating fresh tumor samples from tumor patients into immunodeficient mice, and the tumor samples are divided and proliferated in the physiological environment of the mice. The PDX model retains the heterogeneity, pathological characteristics, gene expression and variation of tumor patients, and has a high clinical correlation with patients, so it has advantages that other tumor models cannot match. Significant. However, low success rate, long cycle time, and high cost are important bottlenecks that hinder the widespread use of PDX models. [0003] The high morbidity and mortality of lung cancer make it one of the leading caus...

AI Technical Summary

Problems solved by technology

This method usually requires expensive small animal-specific imaging equipment (such as CT, ultrasound), and requires professional knowledge of small animal imaging and complex operations.

Ultrasound probes for small animals are relatively large, so they are not suitable for ultrasound-guided puncture of organs such as mice, which are relatively small and have poor ultrasound imaging.

Small animal CT detection needs to take immunodeficient animals out of the SPF animal room, and there is radioactivity, usually there is no suitable operating space, so the practical feasibility is very small

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