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A kind of g3bp2 polypeptide vaccine and its application

A polypeptide vaccine and vaccine technology, applied in the field of biomedicine, can solve the problems of no relevant reports on cardiovascular diseases, achieve the effects of lowering blood sugar levels, high safety, and reducing the level of inflammatory factors

Active Publication Date: 2021-08-27
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that G3bp2 is closely related to the initiation of cancer, viral infection, and the occurrence of neurological diseases, but there is no related report in cardiovascular diseases

Method used

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  • A kind of g3bp2 polypeptide vaccine and its application
  • A kind of g3bp2 polypeptide vaccine and its application
  • A kind of g3bp2 polypeptide vaccine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of immune antigen emulsion

[0036] Before immunization, 1 mg of KLH-G3bp2 or KLH was dissolved in 1 ml of sterile PBS buffer, and sterilized by filtering with a 0.2 μm filter head before use.

[0037] Specific preparation of antigen emulsion: Accurately draw 20 μl of KLH-G3bp2 or KLH solution, 120 μl of Freund’s adjuvant, and 120 μl of normal saline, add to a sterilized centrifuge tube and shake and emulsify for 30 minutes (emulsify for 20 seconds each time, then place on ice for 1 minute). After the emulsification is complete, draw a drop of liquid and drop it on the cold water surface. If it keeps the shape of a ball for a long time and does not disperse when shaken gently, it indicates that it has been fully emulsified. The first two immunizations were emulsified with Freund's complete adjuvant, and the last three times were emulsified with Freund's incomplete adjuvant.

Embodiment 2

[0039] Mouse immunization and harvesting

[0040] After the mice were fed with high fat for 8 weeks, blood was taken from the orbit, and then subcutaneous immunization was repeated 5 times in 12 subcutaneous locations on the abdomen and back. At the 4th week after the initial immunization, blood was taken from the eyeball after isoflurane anesthesia, and then the mice were killed by neck dislocation , and harvest aorta, heart, liver, spleen, lung, and kidney samples.

Embodiment 3

[0042] Synthetic peptide-coated sulfhydryl-binding plate and evaluation of immune effect

[0043] 1. Buffer preparation

[0044] 1) Coating buffer

[0045] Weigh 1.59g of sodium carbonate and 2.93g of disodium bicarbonate, dissolve them in 1000ml of double-distilled water, mix well, adjust the pH to 9.1, and store them at 4°C for later use.

[0046] 2) Wash buffer

[0047] Weigh 0.2g potassium dihydrogen phosphate, 0.2g disodium hydrogen phosphate dodecahydrate, 0.8g sodium chloride, 0.2g potassium chloride, measure 0.05% Tween-20 0.5ml, then add double distilled water to 1000ml .

[0048] 3) Blocking buffer

[0049] Weigh 5g of bovine serum albumin, add double distilled water to 100ml, mix well and store in 4°C for later use.

[0050] 4) Stop solution

[0051] Take 89.15ml of double distilled water, slowly add 10.85ml of 98% concentrated sulfuric acid into it dropwise along the wall, and stir gently while adding.

[0052]2. Operation steps

[0053] 1) Coating: Dilute ...

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Abstract

The invention belongs to the field of biomedicine, and in particular relates to a G3bp2 polypeptide vaccine and its application. The invention provides a G3bp2 polypeptide vaccine for subcutaneous immunization injection, which can stimulate the production of a large amount of specific antibodies against G3bp2 protein in mice, reduce the level of inflammatory factors in the blood, and can effectively inhibit the development of atherosclerosis. Current treatments of atherosclerosis offer new targets and approaches. In addition, the vaccine provided by the invention can also reduce the blood sugar level and the ratio of total hemoglobin to albumin, indicating that it may also have potential curative effect on diabetes and neurological diseases. Finally, the vaccine provided by the invention has high safety and important clinical value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a G3bp2 polypeptide vaccine and its application. Background technique [0002] G3BP (Ras-GTPase-activating protein SH3domain binding protein) is a highly conserved RasGAP-binding protein in the evolution of eukaryotes, mainly localized in the cytoplasm. It belongs to the family of RNA-binding proteins, of which there are at least three in mammals: G3bp1, G3bp2a, and G3bp2b. Many evidences have shown that G3BP is involved in various cell signaling pathways and RNA metabolism; it is overexpressed in various tumor tissues and cells, suggesting that G3BP may be a tumor marker and may be related to the occurrence and development of tumors. [0003] Stress granules (SG) are granules formed in the cytoplasm when eukaryotic cells are subjected to various physical and chemical stimuli, and are an adaptive protection mechanism. A large number of studies have found that the formatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K39/385A61K38/10A61P9/10A61P3/10A61P25/00
CPCA61K9/0019A61K38/10A61K39/00A61K39/385A61K2039/54A61K2039/6081A61P3/10A61P9/10A61P25/00
Inventor 王贵学李天函邱菊辉
Owner CHONGQING UNIV
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