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Exosome mouth wash as well as preparation method and application thereof

An exosome and mouthwash technology, applied in the field of oral ulcer medicine, can solve problems such as bacterial invasion, normal oral flora imbalance, and lower human immunity, so as to promote epithelial cell regeneration, shorten the time for oral mucosal tissue repair, Effects that contribute to a normal diet

Inactive Publication Date: 2021-06-04
陕西中鸿科瑞再生医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Antibiotics are used most frequently in the medicines for the treatment of oral ulcers in the prior art. Although antibiotics are beneficial to reduce the amount of bacterial reproduction and give full play to the disinfection and sterilization effect, long-term use will affect the normal flora imbalance of the oral cavity, which will reduce human immunity in the long run and increase The danger of bacterial invasion

Method used

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  • Exosome mouth wash as well as preparation method and application thereof
  • Exosome mouth wash as well as preparation method and application thereof
  • Exosome mouth wash as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A preparation method of exosome mouthwash, comprising the steps of:

[0034] (1) Preparation of mesenchymal stem cell exosome solution:

[0035] S1. Sampling: Take the isolated umbilical cord tissue in a sterile state, wash it with PBS with a volume fraction of 1% double antibody, wash away the blood stains in the isolated umbilical cord tissue, remove the arteries and veins in the tissue, and peel off the Huahua in the tissue. Tong’s jelly, until the umbilical cord tissue’s HBV, EBV, HIV and CMV test results are qualified, shredded to 1mm 3 , to obtain tissue block samples;

[0036] S2. Adhesive culture: culture the tissue block sample in step S1, place the tissue block sample in 15 mL of α-MEM medium with a volume fraction of 10% FBS, at 37°C, 5% CO 2 Cultivate in a cell incubator for 12-15 days to obtain adherent cells;

[0037] S3. Subculturing: Discard the cell supernatant in step S2, wash the cells with PBS containing 1% double antibody by volume, the double an...

Embodiment 2

[0043] The preparation steps are the same as in Example 1, except that the ratio of raw materials is replaced by 96% of mesenchymal stem cell exosome solution, vitamin B 2 1.7%, lidocaine 2.3%, the exosome concentration in the mesenchymal stem cell exosome solution is 0.2mg / mL.

Embodiment 3

[0045] The preparation steps are the same as in Example 1, except that the ratio of raw materials is replaced by 96% of mesenchymal stem cell exosome solution, vitamin B 2 2%, lidocaine 2%, and the exosome concentration in the mesenchymal stem cell exosome solution is 0.3mg / mL.

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Abstract

The invention relates to the technical field of oral ulcer medicines, in particular to exosome mouth wash as well as a preparation method and application thereof. The exosome mouth wash is prepared from raw materials in parts by weight: 95-97% of a mesenchymal stem cell exosome solution, 1.5-2.5% of vitamin B2 and 1.5-2.5% of lidocaine. The mesenchymal stem cell exosome solution extracted from umbilical cord mesenchymal stem cells is compounded with vitamin B2 and lidocaine, so that the adhesion effect of the mesenchymal stem cell exosome can be improved, the local microenvironment of the oral cavity is effectively improved, and the repairing time of oral mucosa tissues is shortened.

Description

technical field [0001] The invention relates to the technical field of oral ulcer drugs, in particular to an exosome mouthwash and its preparation method and application. Background technique [0002] Oral ulcers are very common clinically, also known as "oral sores". Patients suffering from this disease will clinically show adverse symptoms such as loss of appetite, congested ulcers, and local swelling and pain. The recurrence rate of this disease is high and it is not easy to recover If the long-term treatment effect is poor, it may induce chronic ulcers, and even affect the patient's normal diet and expression functions. In severe cases, it may induce precancerous lesions, and even damage the patient's life safety. [0003] The oropharynx is the channel connecting the oral cavity, nasopharynx, lower respiratory tract, esophagus, etc., and communicates with the external environment. It is the physiological site where many bacteria invade the lower respiratory tract and lun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/98A61K8/67A61K8/42A61Q11/00A61K35/28A61P1/02A61K31/525A61K31/167
CPCA61K8/99A61K8/982A61K8/673A61K8/42A61Q11/00A61K35/28A61K31/525A61K31/167A61P1/02A61K2800/591
Inventor 马嘉丽明磊国王清霞
Owner 陕西中鸿科瑞再生医学研究院有限公司
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