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A kind of cellulose degrading bacterium and application

A technology of cellulose-degrading bacteria and inoculants, applied in fungi, microorganisms, microorganisms, etc., can solve the problems of increasing pathogenic bacteria, seeds not being in good contact with soil, affecting germination and growth, and achieving good degradation effect.

Active Publication Date: 2021-09-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, during the planting process, the seeds cannot be in good contact with the soil. If watering is difficult or the weather is bad, it will cause the seeds, especially small particles like wheat, to fail to take root, affecting normal germination and growth.
At the same time, straw often carries pathogenic bacteria, and the residue of straw returned to the field increases the number of pathogenic bacteria, increases the chance of pathogenic bacteria spreading, and often leads to serious diseases

Method used

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  • A kind of cellulose degrading bacterium and application
  • A kind of cellulose degrading bacterium and application
  • A kind of cellulose degrading bacterium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Sampling

[0029] Soil samples from wheat-corn-wheat rotation fields in Henan Province and 10 parts, each 1000g, wheat-peanut-wheat rotation field soil samples (10 parts, each 1000g). Remove the weeds on the soil and ground, randomly take 20cm soil samples below the surface, put them in sterile bags, and store them at 4°C after sealing.

[0030] 2. Screening of cellulose degrading bacteria

[0031] Pour the cellulose screening medium (PSM) into a 9 cm diameter Petri dish and air dry it for later use. Prepare a soil suspension. Weigh 1g of each of the 20 kinds of soil samples, pour them into a clean test tube, add 9ml of sterile water, shake on a water shaker, and make 1×10 -1 g / L soil suspension, then serially diluted to 1×10 -2 , 1×10 -3 , 1×10 -4 and 1×10 -5 soil suspension. Take 200 μL of the serially diluted suspension and evenly spread it on the cellulose selection medium. Blow dry on a sterile operating bench and seal. Invert the medium coated with so...

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Abstract

The invention discloses a cellulose-degrading bacterium and its application. The cellulose-degrading bacterium is named Talaromyces amestolkiae, the strain number is P5, and the preservation number is CCTCC M 2020111. The present invention obtains a bacterial strain capable of efficiently degrading cellulose through screening, has a better degradation effect on crop straws, and can be used for biodegrading crop straws.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cellulose-degrading bacterium and its application. Background technique [0002] The largest proportion of crops is crop straw. After the crops are mature, after harvesting the above-ground seeds, the remaining residues, that is, straw. About 2 billion tons of straw are produced globally every year. Straw utilization can be divided into five categories (Bu Yujian et al., 2006), one is used as raw material for papermaking, the other is used as base material for cultivation of edible fungi, the third is used as feed for livestock, the fourth is used as fuel, and the fifth is used as fertilizer. Among them, the amount used for papermaking or mushroom cultivation substrate is very small, and the proportion is low. The straw used in animal husbandry is mainly the relatively high-quality part of the straw, and the amount used is not large. Straw has been used as living fuel for a lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 张敬泽刘欣冉郭赛赛何水平
Owner ZHEJIANG UNIV