A kind of microecological preparation and preparation method of coupled fermentation of bacteriophage and probiotics
A microbial preparation and bacteriophage technology, applied in the field of microbial preparations, can solve problems such as in-depth technical research, and achieve the effects of reducing production costs, good application prospects, and strengthening synergy.
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[0052] For the preparation method of probiotics coupled with phage and probiotic fermentation, see figure 1 , the steps are as follows:
[0053] Step 1, preparation of seed solution: insert the activated host strain into TSB medium, the inoculation ratio is 1-20%, the culture temperature is 25-40°C, the rotation speed is 20-240rpm, and the culture time is 4-30h, and the host is obtained Bacteria seed liquid; insert the activated probiotic strains into the seed medium, the inoculation ratio is 1-20%, the culture temperature is 25-40°C, the rotation speed is 20-240rpm, and the culture time is 4-30h, to obtain the probiotic seed liquid .
[0054] Step 2, fermentation culture: inoculate the phage and host bacteria seed liquid into the fermentation medium, and ferment for 4-30 hours to obtain the phage fermentation liquid. The fermentation liquid is sterilized by a 0.22 μm membrane, and the probiotic seed liquid is inserted into the sterile phage In the fermentation liquid, ferme...
Embodiment 2
[0073] A microecological preparation and preparation method for coupled fermentation of Staphylococcus aureus phage and Enterococcus faecium, the steps of which are as follows:
[0074] Step 1, seed liquid preparation: insert the activated Staphylococcus aureus host strain into TSB medium, the inoculation ratio is 0.5%, the cultivation temperature is 32°C, the rotation speed is 200rpm, and the cultivation time is 6h, to obtain the Staphylococcus aureus host Bacteria seed solution: Inoculate the activated Enterococcus faecium strain into the seed medium with an inoculation ratio of 3%, culture temperature at 37° C., rotation speed at 190 rpm, and cultivation time for 18 hours to obtain Enterococcus faecium seed solution.
[0075] Step 2, fermentation culture: inoculate the Staphylococcus aureus phage and the seed liquid of the host bacteria into the fermentation medium, and ferment for 10 hours to obtain the Staphylococcus aureus phage fermentation liquid, the fermentation liqui...
Embodiment 3
[0083] A kind of microecological preparation and preparation method of coliphage phage coupled fermentation with plantaractobacillus and bacillus licheniformis, its steps are as follows:
[0084] Step 1, seed liquid preparation: insert the activated Escherichia coli host strain into TSB medium, the inoculation ratio is 15%, the cultivation temperature is 34°C, the rotational speed is 210rpm, and the cultivation time is 8h, to obtain the Escherichia coli host strain seed liquid; Insert the activated Lactobacillus plantarum into the seed medium, the inoculation ratio is 12%, the culture temperature is 36°C, the rotation speed is 80rpm, and the culture time is 12h, to obtain the plantarum Lactobacillus and seed liquid; the activated Bacillus licheniformis The seeds were inserted into the seed medium, the inoculation ratio was 14%, the cultivation temperature was 34° C., the rotational speed was 180 rpm, and the cultivation time was 19 hours, so as to obtain the Bacillus lichenifor...
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