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Test strip and method for detecting procymidone

A technology of procymidone and test strips, which is applied to the detection of procymidone residues in vegetables and fruits, and the field of colloidal gold test strips, can solve the problem of difficult to meet the rapid detection of a large number of samples and on-site samples, cumbersome and time-consuming processing, and detection costs. High-level problems, to achieve the effect of short detection time, easy promotion and use, and high sensitivity

Pending Publication Date: 2020-11-20
BEIJING KWINBON BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods must be operated under laboratory conditions. The sample pretreatment is cumbersome and time-consuming, and expensive instruments and equipment are required. The detection cost is high, time-consuming, and the operation is complicated. There are great limitations in the actual application process, and it is difficult to meet The need for rapid detection of a large number of samples and on-site samples

Method used

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  • Test strip and method for detecting procymidone
  • Test strip and method for detecting procymidone
  • Test strip and method for detecting procymidone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 detects the preparation of the test paper strip of procymidone

[0043] The preparation method of this test strip mainly comprises the following steps:

[0044] 1) Prepare a conjugate release pad sprayed with procymidone monoclonal antibody-colloidal gold marker;

[0045] 2) preparing a reaction membrane with a detection line coated with procymidone hapten I-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0046] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0047] The following step-by-step detailed description:

[0048] 1. Synthesis of procymidone hapten Ⅰ (see the appendix for the synthetic route figure 2 )

[0049]Dissolve 2.99g of Pythium hydroxypyridium in 80mL of pyridine, add 1.1g of succinic anhydride, heat in an oil bath, and react at 75°C for 6h. Extract, collect...

Embodiment 2

[0090] The detection of procymidone in embodiment 2 vegetables and fruits

[0091] 1. Sample pretreatment

[0092] Return the sample to room temperature (20-30°C) before testing, take a fresh sample to wipe off the soil, and cut it into pieces smaller than 1cm square; weigh (1.00±0.05)g sample into a 15mL polystyrene centrifuge tube, then add 6mL of extraction solution, cover the lid, shake manually for 30s, and let it stand for 1min to obtain the sample solution; according to the detection requirements, dilute according to the following table to obtain the sample solution to be tested.

[0093] sample name National standard limit (mg / kg) Sample solution (μL) + extract solution (μL) Chinese chives 0.2 No need to dilute, it is the sample to be tested tomato, cucumber 2 100+900 eggplant, peppers, grapes 5 50+950 strawberry 10 20+660

[0094] The extract is 0.02mol / L phosphate buffer solution with pH 7.0.

[0095] 2. Test with test...

Embodiment 3

[0101] Example 3 sample detection example

[0102] 1. Detection limit test

[0103] Take blank samples of leek, tomato, cucumber, eggplant, pepper, grape, and strawberry, add procymidone to them respectively until the final concentration is 1 / 2, 1 time, and 2 times the limit of the national standard, and take a test strip for detection. Samples were measured in triplicate.

[0104] When testing samples of leek, tomato, cucumber, eggplant, pepper, grape, and strawberry with a test strip, when there is no procymidone in it and the concentration is 1 / 2 of the national standard limit, the T line color ratio will be displayed on the test strip The color of the C line is dark or consistent with the color of the C line, which is negative; when the added concentration of procymidone is 1 and 2 times the national standard limit, the color of the T line is lighter than that of the C line on the test strip Or the T line does not show color and is positive, indicating that the detection...

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Abstract

The invention discloses a test strip and a method for detecting procymidone. The test strip comprises a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottom plate, wherein the reaction film is provided with a detection line coated with a procymidone hapten I-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody; a procymidone monoclonal antibody-colloidal gold marker is sprayed on the conjugate release pad, and the procymidone monoclonal antibody is prepared by taking a procymidone hapten II-carrier protein conjugate as an immunogen. The invention also provides a method for detecting procymidone in vegetables and fruits by using the test strip. The test strip provided by the invention has the advantages of simplicity in operation, high sensitivity, high detection speed, low cost, suitability for large-batch sample screening and the like, and can meet the requirements of food supervision departments in China for carrying out on-site monitoring and detection work.

Description

technical field [0001] The invention relates to a test strip and a method for detecting procymidone, in particular to a colloidal gold test strip for detecting procymidone, which is particularly suitable for detecting procymidone residues in vegetables and fruits. Background technique [0002] Procymidone is a broad-spectrum systemic low-toxic fungicide, mainly used for the prevention and treatment of botrytis, sclerotinia, brown rot, large leaf spot and scab on fruits, vegetables, flowers and other plants . Procymidone has a strong stimulating effect on the normal synthesis and metabolism of the skin and eyes. Once it enters the human body, it will interfere or inhibit the endocrine system of the substance and have an impact on human health. my country's national standard GB 2763-2019 "National Food Safety Standard Maximum Residue Limits of Pesticides in Food" stipulates that the residue limit of procymidone in vegetables and fruits is 0.2-10mg / kg. [0003] The reported d...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N33/532G01N33/53
CPCG01N33/577G01N33/558G01N33/543G01N33/532G01N33/5308
Inventor 吴小胜马玉华崔海峰王健朱亮亮刘玉梅何方洋冯才伟
Owner BEIJING KWINBON BIOTECH
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