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A bionic intestinal organ chip and its preparation method and application

An organ chip and chip technology, applied in the field of organ bionics, can solve the problems that the uniformity of the villi structure cannot be guaranteed, the inconvenience of experiment data statistics and result comparison, etc., achieve good reproducibility, and reduce the difference in intestinal tissue structure Effect

Active Publication Date: 2022-02-22
SHENZHEN HUADA GENE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the disadvantage of the gut-on-a-chip in the prior art is that the uniformity of the villus structure spontaneously formed by the cells cannot be guaranteed, resulting in large differences between different batches of the chip, which is not convenient for the statistics of experimental data and the comparison of results.

Method used

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  • A bionic intestinal organ chip and its preparation method and application
  • A bionic intestinal organ chip and its preparation method and application
  • A bionic intestinal organ chip and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] This embodiment provides a bionic intestinal organ chip. The bionic intestinal organ chip has a three-layer structure, which simulates the dynamic microenvironment in the intestinal tract and is used for dynamic cultivation of intestinal cells. It is suitable for intestinal diseases, drug screening, and food safety. And so on.

[0026] like figure 2 As shown, there is a fluid channel in the bionic intestinal organ chip of this embodiment. Specifically, the chip includes an upper chip 210 and a lower chip 220 . The lower surface of the upper chip 210 has an open upper fluid channel, the upper surface of the lower chip 220 has an open lower fluid channel, the porous membrane 230 is sealed between the upper chip 210 and the lower chip 220, and the porous membrane 230 is surrounded by the upper chip 210. The upper layer fluid channel 211 is synthesized, and the lower layer fluid channel 221 is surrounded by the porous membrane 230 and the lower layer chip 220 . Several ...

Embodiment 2

[0033] This embodiment provides a method for preparing a bionic intestinal organ chip. The preparation method mainly adopts soft lithography technology to prepare the bionic intestinal organ chip in the first embodiment above.

[0034] like image 3 As shown, the preparation method of the bionic intestinal organ chip of this embodiment mainly includes the following steps:

[0035] S100: preparing an upper chip and a lower chip;

[0036] S200: preparing a porous membrane template;

[0037] S300: preparing a porous membrane and sealing and assembling.

[0038] In step S300, a porous membrane is prepared by using a porous membrane template, and the porous membrane is sealed between the upper chip and the lower chip to form a bionic intestinal organ chip. Wherein, the produced porous membrane is distributed with several protrusions for simulating the structure of intestinal villi and several through holes for simulating the intestinal absorption function.

[0039] In the prepa...

Embodiment 3

[0086] This embodiment provides a method for preparing a bionic intestinal organ. This preparation method is carried out through the bionic intestinal organ chip described in Example 1. This method is an application to the bionic intestinal organ chip.

[0087] In this example, three-dimensional intestinal microtissues were constructed and dynamically cultured in the bionic intestinal organ chip, such as Figure 8 As shown, it specifically includes the following steps:

[0088] S401: Sterilize the bionic intestinal organ chip;

[0089] Using 70% alcohol and ultraviolet rays to sterilize the bionic intestinal organ chip described in Example 1;

[0090] S402: injecting the extracellular matrix solution into the fluid channel, modifying the porous membrane, and cleaning the fluid channel after modification;

[0091] Inject the extracellular matrix (type I collagen, matrigel, etc.) solution from the inlet of the upper chip into the channel to modify the PDMS porous membrane, and...

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Abstract

The application discloses a bionic intestinal organ chip and its preparation method and application. The bionic intestinal organ chip has a fluid channel, and a porous membrane is arranged in the fluid channel, and the porous membrane separates the fluid channel into an upper fluid channel and a lower fluid channel , the porous membrane is distributed with several protrusions for simulating the villus structure of the intestine and several through holes for simulating the absorption function of the intestine. The upper fluid channel is used to cultivate intestinal cells, the lower layer is used to collect metabolites, the protrusions on the porous membrane are used to simulate the structure of intestinal villi, and the through holes are used to simulate the absorption function of the intestinal tract. Since several raised three-dimensional scaffold structures are distributed on the porous membrane in the chip, the difference in intestinal tissue structure between different batches of chips can be greatly reduced, and the chip has good reproducibility. The bionic intestinal organ chip can use perfusion to dynamically culture intestinal cells to simulate the dynamic microenvironment in the intestinal tract, and is suitable for research on intestinal diseases, drug screening, food safety, etc.

Description

technical field [0001] The present application relates to the technical field of organ bionics, in particular to a bionic intestinal organ chip and its preparation method and application. Background technique [0002] Microfluidics, as one of the important cutting-edge technologies in the 21st century, provides an important platform for simulating human metabolism models in vitro. It is mainly based on micro-nano processing technology, which forms a network of micron-scale channels and runs through the entire system with controllable fluids, which can realize the routine functions of biology and chemistry laboratories. Because of its micron-sized components that match the size of the cells, various cell cultures and fluid stimulation can be performed in the microchannel of the chip, and a three-dimensional microenvironment that is close to the physiological environment and has the characteristics of time and space resolution has become an important tool in the construction o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/22C12M1/00C12M1/16
CPCC12M1/16C12M1/00C12M3/00
Inventor 魏文博陈娟娟肖亮
Owner SHENZHEN HUADA GENE INST
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