Application of naringenin in preparation/protection of renal ischemia reperfusion injury through relieving of renal inflammatory response

A technology of ischemia-reperfusion and inflammatory response, applied in the field of biomedicine, can solve the problems of lack of strong evidence for drug effects, unconfirmed protective effects, and few reports of effects

Active Publication Date: 2020-11-24
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protective effect of naringenin on heart and cerebral ischemia injury has been widely reported, but the protective effect on renal ischemia-reperfusion injury has not been confirmed so far. The only research in the field of kidney is limited to diabetic nephropathy, drug Induced renal injury, rarely reported role

Method used

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  • Application of naringenin in preparation/protection of renal ischemia reperfusion injury through relieving of renal inflammatory response
  • Application of naringenin in preparation/protection of renal ischemia reperfusion injury through relieving of renal inflammatory response
  • Application of naringenin in preparation/protection of renal ischemia reperfusion injury through relieving of renal inflammatory response

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Naringenin pretreatment grouping

[0033] Dissolve the dry powder of naringenin in DMSO to make a mother solution, and dilute it with saline to make a working solution of naringenin. Thirty 8-week-old, healthy male SPF-grade Wistar rats weighing 250-300g were raised in isolated air-supplied cages, the temperature was controlled at 21°C±2°C, and the relative humidity was controlled at 50%±15%, day and night. The cycle is 12 hours, normal diet, and the litter is changed every 2 days. They were randomly divided into 4 groups, namely sham operation group, AKI group, naringenin+AKI group, and DMSO+AKI group, with 6 rats in each group. Rats in the treatment group (naringenin+AKI group) were given 50 mg / kg*d of naringenin by intraperitoneal injection 7 days before modeling; the control group (DMSO+AKI group) was injected in the same way DMSO saline solution with equal concentration and equal volume.

[0034] The AKI group, the naringenin+AKI group and the DMSO+...

Embodiment 2

[0035] Embodiment 2 Ischemia-reperfusion model and sham operation model making

[0036]The Wistar rats of 6-8 weeks were cultured under the conditions of 21°C±2°C, 50%±15% humidity, and 12 hours of light, and were fed with standard pellet feed and sterile water. Rats were kept in a cage of 6 and fasted for 12 hours before surgery. The high-pressure steam sterilizer is set at 120°C for 40 minutes, and the surgical instruments are put in for sterilization. After the rats in the AKI group, naringenin+AKI group and DMSO+AKI group were pretreated, they were anesthetized with pentobarbital sodium (30 mg / kg) by intraperitoneal injection, and the rats were in a state of complete muscle relaxation. Take the supine position upwards, gently fix the limbs with elastic ropes, and pull the elastic ropes to fix the incisors upwards. Because the anesthesia time cannot be determined, the rats may be awake during the operation. If the fixation is not good, the operator may be bitten. Lift the...

Embodiment 3

[0039] Embodiment 3 Rat blood creatinine, blood urea nitrogen monitoring

[0040] Put 5ml of the blood sample taken out above into a 5ml EP tube and let it stand for 1h, centrifuge at 3000rpm for 15min in a high-speed centrifuge, take the upper serum and transfer it to a new EP tube for the detection of serum creatinine and urea nitrogen, and store at 4°C. The specimens were sent to the laboratory department of the hospital within 24 hours, and the serum creatinine level (purchased from Nanjing Jiancheng) was measured by the protein removal method. The test results are shown in Table 1.

[0041] Table 1 Rat serum creatinine (μmol / l) result

[0042]

[0043] According to the results in Table 1, the serum creatinine level in the AKI group was significantly higher than that in the sham operation group, reaching the level of acute kidney injury, indicating that the modeling of this experiment was successful. There was no significant difference in creatinine level between DMSO+...

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Abstract

The invention provides application of naringenin in preparation/protection of renal ischemia reperfusion injury through relieving of renal inflammatory response, and belongs to the field of biologicalmedicines. The application proves that the naringenin is capable of preventing/protecting human acute kidney injury caused due to ischemia reperfusion, and a protection effect of the naringenin is achieved through the relieving of the renal inflammatory response; the application further proves, through bioinformatics, that an action mechanism of the naringenin is relevant with direct bonding of NF-kB associated proteins; and finally, experiments prove that the expression amount of NF-kB is decreased after the application of the naringenin, so that a theoretical basis is provided for broadening the possible application range of the naringenin. The naringenin can be applied to theoretical researches and clinic treatments on the renal ischemia reperfusion injury, thereby providing an idea for exploration of prevention and/or protection effects of traditional Chinese medicines to the acute kidney injury caused due to the ischemia reperfusion.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of naringenin in preventing / protecting renal ischemia-reperfusion injury by alleviating renal inflammatory response. Background technique [0002] Acute kidney injury (Acute kidney injury, AKI) is caused by a variety of etiologies and causes a sudden decline in renal function within a short period of time, with a mortality rate as high as 50%-79%. Studies have shown that the incomplete repair of renal tissue after AKI injury leads to irreversible fibrosis of the kidney, and the proportion of survivors who progress to chronic renal failure reaches 35%-71%. Therefore, in order to slow down and reverse the progress of renal disease and improve renal function, early clinical treatment of renal injury is of great significance to improve the prognosis of patients with renal disease. [0003] Renal ischemia-reperfusion injury (Ischemia reperfusion, IR) is one of t...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P9/10A61P13/12
CPCA61K31/352A61P9/10A61P13/12Y02A50/30
Inventor 徐岩代杰杨成宇
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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