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A method for separating and purifying soybean straw polysaccharide for inhibiting human hepatoma cells and its application

A technology of soybean straw and liver cancer cells, which is applied in the direction of drug combination and antineoplastic drugs, can solve the problems of ineffective application of polysaccharide components and low utilization rate of soybean straw, and achieve the effect of increasing extraction rate and deproteinization rate

Active Publication Date: 2022-06-28
綦峥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problem that the existing soybean straw utilization rate is low and its polysaccharide components have not been effectively used, and provides a soybean straw polysaccharide separation and purification method for inhibiting human liver cancer cells and its application

Method used

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  • A method for separating and purifying soybean straw polysaccharide for inhibiting human hepatoma cells and its application
  • A method for separating and purifying soybean straw polysaccharide for inhibiting human hepatoma cells and its application
  • A method for separating and purifying soybean straw polysaccharide for inhibiting human hepatoma cells and its application

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specific Embodiment approach 1

[0043] Embodiment 1: This embodiment is a method for separating and purifying soybean straw polysaccharide for inhibiting human hepatoma cells, which is specifically carried out according to the following steps:

[0044] 1. Preparation of soybean straw crude polysaccharide;

[0045] ①Dry the soybean straw, crush it with a pulverizer, and then sieve it to obtain coarse straw powder;

[0046] ② Soak the coarse straw powder in water for 12 to 14 hours, and then reflux in a hot water bath to obtain an extract; then filter and concentrate the obtained filtrate under reduced pressure to obtain a concentrate, and the volume ratio of the concentrate to the filtrate is 1:5 ;

[0047] ③ Cool the concentrated solution, add absolute ethanol, precipitate for 12-14 hours, and then centrifuge to obtain a precipitate;

[0048] ④ Freeze-dry the precipitate to obtain soybean straw crude polysaccharide powder;

[0049] 2. Add soybean straw crude polysaccharide powder into distilled water to obt...

specific Embodiment approach 2

[0053] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass concentration of soybean straw crude polysaccharide in the polysaccharide solution in step 2 is 0.25 mg / mL. Others are the same as the first embodiment.

specific Embodiment approach 3

[0054] Embodiment 3: This embodiment is different from Embodiment 1 or 2 in that: in step 2, the mass-volume ratio of crude polysaccharide of soybean straw to Savage reagent is 50 mg: 20 mL; the mass-volume ratio of crude polysaccharide of soybean straw to TCA solution is 50mg: 10mL, the volume percentage of TCA solution is 1%. Others are the same as the first embodiment.

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Abstract

A separation and purification method and application of soybean stalk polysaccharide for inhibiting human liver cancer cells. The invention relates to the field of separation and purification method and application of polysaccharide. The invention aims to solve the technical problem that the utilization rate of the existing soybean stalk is low and the polysaccharide component thereof has not been effectively used. Methods: Preparation of crude polysaccharide; deproteinization; decolorization; separation by DEAE‑52 cellulose column chromatography; separation by Sephadex G‑100 gel column chromatography. The present invention optimizes the separation and purification method of soybean straw polysaccharides, uses the Savage method combined with the trichloroacetic acid method to carry out deproteinization treatment on soybean straw polysaccharides, improves the deproteinization rate, and greatly improves the yield of soybean straw polysaccharides through subsequent separation and purification operations extraction rate. Applying soybean straw polysaccharides to antineoplastic drugs for inhibiting liver cancer cells can also improve the utilization rate of soybean straw and reduce environmental pollution. The isolated and purified soybean stalk polysaccharide of the invention is used as a bioactive component for inhibiting liver cancer cells to prepare antitumor drugs.

Description

technical field [0001] The invention relates to a polysaccharide separation and purification method and application field. Background technique [0002] my country is a big agricultural country, in which the soybean industry is developing rapidly, and the output is increasing year by year. However, while bringing benefits, it will also generate a large amount of straw that is difficult to handle. At present, the utilization rate of soybean straw is less than 3%, which shows that my country's soybean straw resources are still large. Potential to use. In particular, the polysaccharide components contained in soybean straw have not been deeply studied. The biological activity of polysaccharides is affected by the configuration of polysaccharides at all levels, and will show different active states, thereby affecting life activities. Development and utilization are of great value. Soybean straw is often discarded as waste, and the structural characteristics and pharmacological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00
CPCC08B37/0003C08B37/006A61P35/00
Inventor 綦峥于淼王冰
Owner 綦峥