Method for releasing bionic magnetic vesicles based on macrophages and application of bionic magnetic vesicles

A macrophage and magnetic technology, applied in the field of biomedical materials, can solve the problems of protein loss, affecting physiological characteristics, cell membrane structure damage, etc., and achieve the effect of simple conditions, good magnetic response performance, and good dispersion

Active Publication Date: 2020-11-27
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such a binding method will inevitably destroy the structure of the cell membrane, and at the same time, the protein embedded in the cell membrane will also be lost, which will affect the physiological characteristics.

Method used

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  • Method for releasing bionic magnetic vesicles based on macrophages and application of bionic magnetic vesicles
  • Method for releasing bionic magnetic vesicles based on macrophages and application of bionic magnetic vesicles
  • Method for releasing bionic magnetic vesicles based on macrophages and application of bionic magnetic vesicles

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Embodiment 1

[0048] A preferred embodiment of the present invention provides a method for releasing biomimetic magnetic vesicles based on macrophages, the specific steps are as follows:

[0049] (1) Superparamagnetic Fe3O4 nanoparticles (Fe 3 o 4 NPs) preparation

[0050] FeCl 3 ·6H 2 O was dissolved in ethylene glycol, and sodium citrate (Na 3 CT), and finally ammonium acetate (NH 4 Ac), after magnetically stirring for 1 h, the mixture was transferred to a stainless steel autoclave, and kept at 200° C. for 16 h. After cooling, the sediment was collected by magnetic separation, washed thoroughly with ethanol and deionized water 4 times, and finally magnetically separated to obtain Fe 3 o 4 Nanoparticles Superparamagnetic Fe3O4 nanoparticles; where FeCl 3 ·6H 2 O, Na 3 CT, NH 4 The mass ratio of Ac is 2.89:1:8.26. Determination of particle size and surface potential of drug-loaded hybrid nanoparticles by Malvern laser particle size analyzer, Fe 3 o 4 The particle size distri...

Embodiment 2

[0060] A preferred embodiment of the present invention provides a method for releasing biomimetic magnetic vesicles based on macrophages, the specific steps are as follows:

[0061] (1) Superparamagnetic Fe3O4 nanoparticles (Fe 3 o 4 NPs) preparation

[0062] FeCl 3 ·6H 2 O was dissolved in ethylene glycol, and sodium citrate (Na 3 CT), and finally ammonium acetate (NH 4 Ac), after magnetically stirring for 2 h, the mixture was transferred to a stainless steel autoclave, and kept at 200° C. for 18 h. After cooling, the sediment was collected by magnetic separation, washed thoroughly with ethanol and deionized water three times, and finally magnetically separated to obtain Fe 3 o 4 Nanoparticles Superparamagnetic Fe3O4 nanoparticles; where FeCl 3 ·6H 2 O, Na 3 CT, NH 4 The mass ratio of Ac is 2.93:1:8.51.

[0063] (2) Culture of mouse macrophages (J774A.1)

[0064] Mouse macrophage J774A.1 was cultured in DMEM complete medium containing 10wt% fetal bovine serum an...

Embodiment 3

[0072] A preferred embodiment of the present invention provides a method for releasing biomimetic magnetic vesicles based on macrophages, the specific steps are as follows:

[0073] (1) Superparamagnetic Fe3O4 nanoparticles (Fe 3 o 4 NPs) preparation

[0074] FeCl 3 ·6H 2 O was dissolved in ethylene glycol, and sodium citrate (Na 3 CT), and finally ammonium acetate (NH 4 Ac), after magnetically stirring for 1 h, the mixture was transferred to a stainless steel autoclave, and kept at 200° C. for 17 h. After cooling, the sediment was collected by magnetic separation, washed thoroughly with ethanol and deionized water 4 times, and finally magnetically separated to obtain Fe 3 o 4 Nanoparticles Superparamagnetic Fe3O4 nanoparticles; where FeCl 3 ·6H 2 O, Na 3 CT, NH 4 The mass ratio of Ac is 2.86:1:8.37.

[0075] (2) Culture of mouse macrophages (J774A.1)

[0076] Mouse macrophage J774A.1 was cultured in DMEM complete medium containing 10wt% fetal bovine serum and 1w...

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Abstract

The invention discloses a method for releasing bionic magnetic vesicles based on macrophages and application of the bionic magnetic vesicles. The method comprises the following steps: S1, preparing superparamagnetic ferroferric oxide nanoparticles; S2, culturing macrophages; S3, co-incubating the macrophages and the superparamagnetic ferroferric oxide nanoparticles; S4, continuing to culture the macrophages; and S5, collecting the bionic magnetic vesicles. After the superparamagnetic ferroferric oxide nanoparticles and the macrophages are incubated, the bionic magnetic vesicles are generated via the macrophages in a way similar to release of exosomes, the performance of a phospholipid membrane is shown outside the vesicles, and meanwhile, the bionic magnetic vesicles have good magnetic response performance and have the potential of being applied to the field of biomedicine.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a method and application for releasing bionic magnetic vesicles based on macrophages. Background technique [0002] Magnetic nanoparticles have attracted considerable attention in recent years for their wide-ranging applications in technology and biomedicine. Magnetic nanoparticles have broad application prospects in biomedical fields such as disease diagnosis, immunoassay, magnetic resonance imaging (MRI), bioseparation, and biocatalysis. However, on the one hand, when nanometers enter the physiological environment, they will quickly adsorb proteins and other biomacromolecules to form protein halos, thereby changing the size and surface properties of nanoparticles; on the other hand, nanoparticles will also non-specifically adsorb in the physiological environment Cells (such as blood cells) that make it have biological characteristics different from the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0786C01G49/08A61K49/08
CPCC12N5/0645C01G49/08A61K49/08C12N2509/10C01P2004/62
Inventor 吴尧康珂张宇佳朱南行李国浩易强英
Owner SICHUAN UNIV
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