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Bifunctional foot-and-mouth disease immunopotentiator as well as preparation method and application thereof

A technology of immune enhancer and foot-and-mouth disease vaccine, which is applied in the field of biopharmaceuticals, can solve problems such as the absence of cellular immunity, and achieve the effects of improving the level of cellular immunity, improving humoral immune response, and improving efficacy

Inactive Publication Date: 2020-12-01
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no effective cellular immunity. Therefore, in the experiment of optimizing the formula, the efficacy of cellular immunity is emphasized. On the basis of optimizing the formula, adding chitosan can not only stimulate the body to produce antibodies in the early stage, but also significantly improve the immunity after vaccine immunity. The level of humoral and cellular immunity is conducive to the effective clearance of viruses in the presence of viral infections

Method used

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  • Bifunctional foot-and-mouth disease immunopotentiator as well as preparation method and application thereof
  • Bifunctional foot-and-mouth disease immunopotentiator as well as preparation method and application thereof
  • Bifunctional foot-and-mouth disease immunopotentiator as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044]Embodiment 1: different source chitosan immune efficacy comparison

[0045] 1. Experimental materials

[0046] Modified chitosan was purchased from Kuer Chemical Technology (Beijing) Co., Ltd. (product number DS261175); guanidinated chitosan (ZL106905443B); O-carboxymethyl-N-guanidinated chitosan.

[0047] Both muramyl dipeptide (abbreviated as MDP) and hyaluronic acid (500-800 kDa) were purchased from InvivoGen.

[0048] Triphenyl phosphate (TPP) was purchased from Sinopharm Group.

[0049] Astragalus polysaccharide was purchased from Wuhan Xinxinjiali Biotechnology Co., Ltd. (product number 89250-26-0).

[0050] 206 adjuvant was purchased from Sebec.

[0051] Inactivated porcine O-type foot-and-mouth disease virus liquid (the virus strain is porcine O-type foot-and-mouth disease virus Myanmar strain 98), provided by Inner Mongolia Jinyu Group, was inactivated by diethyleneimine, and the 146s content was 5.87 μg / mL.

[0052] The FMD antibody titer of 6-7 week-old he...

Embodiment 2

[0078] The screening of embodiment 2 immunopotentiator formula dosage

[0079] 1. Experimental materials

[0080] O-carboxymethyl-N-guanidine chitosan (prepared in Example 1).

[0081] Both muramyl dipeptide (abbreviated as MDP) and hyaluronic acid (500-800 kDa) were purchased from InvivoGen.

[0082] Triphenyl phosphate (TPP) was purchased from Sinopharm Group.

[0083] Astragalus polysaccharide was purchased from Wuhan Xinxinjiali Biotechnology Co., Ltd. (product number 89250-26-0).

[0084] 206 adjuvant was purchased from Sebec.

[0085] Inactivated porcine O-type foot-and-mouth disease virus liquid (the virus strain is porcine O-type foot-and-mouth disease virus Myanmar strain 98), provided by Inner Mongolia Jinyu Group, was inactivated by diethyleneimine, and the 146s content was 5.87 μg / mL.

[0086] The FMD antibody titer of 6-7 week-old healthy susceptible piglets was ≤1∶8 in liquid-phase blocking ELISA.

[0087] The invention adopts the FMD liquid phase blocking E...

Embodiment 3

[0105] Example 3 Evaluation of the Immune Efficacy of the Bifunctional Immunopotentiator

[0106] 1. Experimental materials

[0107] O-carboxymethyl-N-guanidine chitosan (prepared in Example 1).

[0108] Both muramyl dipeptide (abbreviated as MDP) and hyaluronic acid (500-800 kDa) were purchased from InvivoGen.

[0109] Triphenyl phosphate (TPP) was purchased from Sinopharm Group.

[0110] Astragalus polysaccharide was purchased from Wuhan Xinxinjiali Biotechnology Co., Ltd. (product number 89250-26-0).

[0111] ISA206 was purchased from Subec.

[0112] Inactivated porcine O-type foot-and-mouth disease virus liquid (the virus strain is porcine O-type foot-and-mouth disease virus Myanmar strain 98), provided by Inner Mongolia Jinyu Group, was inactivated by diethyleneimine, and the 146s content was 5.87 μg / mL.

[0113] The FMD antibody titer of 6-7 week-old healthy susceptible piglets was ≤1∶8 in liquid-phase blocking ELISA.

[0114] The invention adopts the FMD liquid pha...

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Abstract

The invention discloses O-carboxymethyl-N-guanidino chitosan, and also discloses a bifunctional immunopotentiator. The bifunctional immunopotentiator contains 10-500 [mu]g / mL of O-carboxymethyl-N-guanidino chitosan, 10-100 [mu]g / mL of muramyl dipeptide, 4-30 mg / mL of triphenyl phosphate, 1-6 [mu]g / mL of hyaluronic acid and 0.2-2 mg / mL of astragalus polysaccharide. The invention also discloses a preparation method and application of the bifunctional immunopotentiator. The invention further discloses a foot-and-mouth disease vaccine of the bifunctional immunopotentiator and a preparation methodof the foot-and-mouth disease vaccine. After the immunopotentiator is mixed with a foot-and-mouth disease inactivated vaccine for use, the efficacy of the vaccine can be effectively improved, high-level cytokines can be detected in 3 days after immunization, and high-level liquid-phase blocking antibodies can be generated in 7 days after immunization. The immunopotentiator not only can improve humoral immune response after vaccine immunization, but also can significantly improve the cellular immune level after vaccine immunization.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a bifunctional foot-and-mouth disease immune enhancer and its preparation method and application. Background technique [0002] Foot-and-mouth disease (FMD) is an acute and severe infectious disease of artiodactyls caused by foot-and-mouth disease virus. Vaccine immunization is the main method to control and prevent the disease, but the current FMD vaccines have the disadvantages of slow antibody production after vaccine immunization, low antibody level, short duration of antibody and poor protection against mutant strains. [0003] In view of the problems existing in the existing inactivated foot-and-mouth disease vaccines, without changing the manufacturing process of the existing vaccines, by adding an immune enhancer, the antibody level after immunization of the vaccines is improved, thereby optimizing the immune efficacy of the existing vaccines. In order to further reduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/135A61P31/14C08B37/08
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/55583A61P31/14C08B37/003
Inventor 侯立婷秦竹陈瑾郑其升杜露平于晓明张元鹏侯继波
Owner JIANGSU ACAD OF AGRI SCI
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