Glucose control process for increasing yield of L-isoleucine
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A technology of isoleucine and glucose, applied in the field of glucose control technology, can solve problems such as difficulty in product purification
Inactive Publication Date: 2020-12-04
TIANJIN UNIV OF SCI & TECH
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However, as long as there is residual sugar in the tank, the by-products will increase synergistically with the production of L-isoleucine, and the by-products will increase with the increase of the main acid L-isoleucine. bring difficulties
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Embodiment 1
[0026] A glucose control process for increasing the output of L-isoleucine, the specific steps are as follows:
[0027] (1) Activation culture: take out the bacteria preservation tube (Corynebacterium glutamate YILM1504, provided by Metabolic Engineering Laboratory of Tianjin University of Science and Technology) from the -80°C refrigerator, pass two generations on the slope, and perform activation culture to obtain the activated strain .
[0028] The slant medium used includes: peptone 5g / L, beef extract 10g / L, yeast powder 5g / L, corn steep liquor dry powder 20g / L, KH 2 PO 4 1 g / L, MgSO 4 0.2 g / L, NaCl1 g / L, agar powder 25g / L, lysine 0.2g / L, pH=6.8~7.0;
[0029] (2) Seed cultivation: all the activated strains were inoculated into seed tanks to obtain seed liquid.
[0030] The seed medium used includes: glucose 35g / L, MgSO 4 0.6 g / L, KH 2 PO 4 1.5 g / L, (NH4 ) 2 SO 4 1g / L, yeast extract powder 5g / L, corn steep liquor dry powder 35g / L, V B1 0.3mg / L, foam enemy 0.2g / L, ...
Embodiment 2
[0036] A glucose control process for increasing the yield of L-isoleucine, referring to Example 1, the difference is that: the initial base material glucose concentration is 60g / L, and the glucose is added at the maximum sugar supplement rate.
Embodiment 3
[0038] A glucose control process for increasing the yield of L-isoleucine, referring to Example 1, the difference is that the initial bottom material glucose concentration is 90g / L, and the glucose is added at the maximum sugar supplement rate.
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Abstract
The invention provides a glucose control process for increasing the yield of L-isoleucine. The glucose control process is a glucose sub-moderate sugar supplementing process; thalli are divided into four stages, namely an adaptation stage, a thalli expanding culture stage, an acid production peak stage and a decay stage; the thalli are Corynebacterium glutamate, have lysine defect types, and are fermented by adopting a base material high initial sugar; namely, 80-120 g / L of glucose is added to a base material at a time, a low-sugar feeding process is adopted in the middle and later periods, namely the feeding acceleration rate ranges from 5.20 g / (L.h) to 7.47 g / (L.h); and the thallus fermentation condition is dynamically and accurately regulated and controlled, and fermentation conversion of thalli is achieved, According to the method, the problems that the thallus adaptation period is long, the acid production peak period is short, the thallus enters the decay period in advance and thelike are solved, the glucose utilization rate is optimized, the saccharic acid conversion rate is increased, accumulation of by-products is reduced, and the heteroacid content is greatly reduced on the premise that the biomass and the acid production amount are not reduced.
Description
technical field [0001] The invention relates to an amino acid production process, in particular to a glucose control process for increasing the output of L-isoleucine. Background technique [0002] L-isoleucine is an essential amino acid for the human body and one of the three branched-chain amino acids. Because of its special structure and function, it occupies a particularly important position in the metabolism of human life. As one of the amino acid raw materials, it is mainly used for compound amino acid infusion, three-branched chain amino acid infusion, amino acid oral liquid, etc. It is also used for the treatment of diseases such as liver disease, hepatic coma, and weakness. It is a relatively expensive amino acid raw material drug. one. [0003] The industrial production processes of L-isoleucine mainly include: protein hydrolysis, chemical synthesis and fermentation. The production of isoleucine by microbial fermentation can realize large-scale industrial product...
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