Glucose control process for increasing yield of L-isoleucine

Abstract

The invention provides a glucose control process for increasing the yield of L-isoleucine. The glucose control process is a glucose sub-moderate sugar supplementing process; thalli are divided into four stages, namely an adaptation stage, a thalli expanding culture stage, an acid production peak stage and a decay stage; the thalli are Corynebacterium glutamate, have lysine defect types, and are fermented by adopting a base material high initial sugar; namely, 80-120 g / L of glucose is added to a base material at a time, a low-sugar feeding process is adopted in the middle and later periods, namely the feeding acceleration rate ranges from 5.20 g / (L.h) to 7.47 g / (L.h); and the thallus fermentation condition is dynamically and accurately regulated and controlled, and fermentation conversion of thalli is achieved, According to the method, the problems that the thallus adaptation period is long, the acid production peak period is short, the thallus enters the decay period in advance and thelike are solved, the glucose utilization rate is optimized, the saccharic acid conversion rate is increased, accumulation of by-products is reduced, and the heteroacid content is greatly reduced on the premise that the biomass and the acid production amount are not reduced.

Description

technical field [0001] The invention relates to an amino acid production process, in particular to a glucose control process for increasing the output of L-isoleucine. Background technique [0002] L-isoleucine is an essential amino acid for the human body and one of the three branched-chain amino acids. Because of its special structure and function, it occupies a particularly important position in the metabolism of human life. As one of the amino acid raw materials, it is mainly used for compound amino acid infusion, three-branched chain amino acid infusion, amino acid oral liquid, etc. It is also used for the treatment of diseases such as liver disease, hepatic coma, and weakness. It is a relatively expensive amino acid raw material drug. one. [0003] The industrial production processes of L-isoleucine mainly include: protein hydrolysis, chemical synthesis and fermentation. The production of isoleucine by microbial fermentation can realize large-scale industrial product...

AI Technical Summary

Problems solved by technology

However, as long as there is residual sugar in the tank, the by-products will increase synergistically with the production of L-isoleucine, and the by-products will increase with the increase of the main acid L-isoleucine. bring difficulties

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