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Application of gut microbiota in the diagnosis of dystonia

A technology for gut microbes and dystonia, applied in the field of medicine and biology, can solve problems such as unknown relationships

Active Publication Date: 2021-10-19
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the relationship between gut microbiota and dystonia is unknown

Method used

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  • Application of gut microbiota in the diagnosis of dystonia
  • Application of gut microbiota in the diagnosis of dystonia
  • Application of gut microbiota in the diagnosis of dystonia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, detection process

[0068] 1. 16S rRNA genome targeted amplification sequencing analysis of intestinal microbiome in patients with isolated dystonia

[0069] 1. Collect fresh excrement from patients with isolated dystonia and their family members, quickly freeze and extract microbiome DNA,

[0070] 2. Use QIAamp Fast DNA Stool Mini Kit kit from Qiangen to extract microbial nucleic acid, quantify and adjust the final concentration to 1ng / ml,

[0071] 3. Use specific primers for the V4 region of the bacterial 16S rRNA gene to amplify, recover and purify the PCR fragments,

[0072] 4. Using Illumina's Hiseq 2500 for high-throughput sequencing,

[0073] 5. Processing of sequencing data, including quality control of raw data, purification, de-redundancy, etc., and finally high-quality sequence data that can be used for subsequent bioinformatics analysis,

[0074] 6. Bioinformatics analysis, including standardizing or normalizing the data of different samples...

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Abstract

The invention belongs to the field of medical biology and relates to the application of intestinal microorganisms in the diagnosis of dystonia. The purpose of the present invention is to provide the application of the reagent for detecting the expression level of intestinal microorganisms in the preparation of a test kit for diagnosing dystonia, wherein said intestinal microorganisms include: Eubacterium hallii (Eubacterium hallii), Braut's ovale Blautia obeum, Dorea longicatena, Bacteroides vulgatus and Bacteroides plebeius.

Description

technical field [0001] The invention belongs to the field of medical biology and relates to the application of intestinal microorganisms in the diagnosis of dystonia. Background technique [0002] Dystonia is a complex, highly variable neurological movement disorder characterized by continuous or intermittent muscle contractions. According to statistics, the prevalence of dystonia is 16 per 100,000, second only to essential tremor and Parkinson's disease, and is the third most common movement disorder. Dystonia can be a manifestation of many neurological disorders, isolated dystonia or a comorbid symptom of other disorders. Isolated dystonia is a dystonic syndrome in which dystonia is the only phenotype other than tremor. Currently, several genes have been shown to be associated with childhood and adolescent-onset dystonia, but most common adult-onset dystonias are often sporadic and have no clear etiology. [0003] The gut microbiome is a complex community of microorgani...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/689C12Q1/6869C12Q1/04G01N30/02G16B50/10G16B30/10A61K45/00A61P21/00A23L33/10
CPCA23V2002/00A61K45/00A23L33/10A61P21/00C12Q1/6869C12Q1/6883C12Q1/689C12Q2600/158G01N30/02G16B30/10G16B50/10C12Q2535/122C12Q2537/165C12Q2531/113A23V2200/316
Inventor 胡永峰马凌燕程敏杨帆金奇
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI