Polystyrene hydrophilic fluorescent microsphere preparation method, prepared microsphere and application
A technology of polystyrene microspheres and fluorescent microspheres, applied in the field of medical materials, can solve the problems of weak reaction signal, influence detection sensitivity, low protein loading and the like, achieve simple raw materials, high detection sensitivity and stability, and process steps. simple effect
Active Publication Date: 2020-12-08
南京基蛋生物医药有限公司
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Problems solved by technology
[0005] This application provides the preparation method of polystyrene hydrophilic fluorescent microspheres, the prepared microspheres and their applications, in order to solve the problem of large amounts of non-specific adsorption and low protein loading when existing carboxyl fluorescent microspheres are used for the detection of amino biomolecules. The reaction signal is weak during the detection process, which affects the detection sensitivity
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[0033] figure 1 It is the flow chart of the example of the polystyrene hydrophilic fluorescent microsphere preparation method of the present application, such as figure 1 Shown, the preparation method of polystyrene hydrophilic fluorescent microspheres comprises:
[0034] Step S100, preparing polystyrene microspheres modified with glycidyl methacrylate (hereinafter abbreviated as GMA).
[0035] In the present application, step S100, preparing GMA-modified polystyrene microspheres, specifically includes,
[0036] Step S110, adding polystyrene microspheres to the mixed reagent of concentrated sulfuric acid and nitric acid, stirring and reacting at 20-40°C for 15-25h, so that the surface of the polystyrene microspheres bears nitro groups, and washing by centrifugation to produce Get nitro microspheres. In this example, the mass ratio of polystyrene microspheres, concentrated sulfuric acid, and nitric acid is 1:(1-1.5):(2-3). It should be noted that those skilled in the art ca...
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Abstract
The invention discloses a preparation method of a polystyrene hydrophilic fluorescent microsphere, the prepared microsphere and application thereof. The preparation method comprises the steps that GMAsurface modification is conducted on the polystyrene microspheres, then fluorescent dyeing is conducted, a polymerization reaction is conducted by adding methacrylic acid, and the polystyrene hydrophilic fluorescent microsphere of which the surface is provided with a carboxyl polymerization coating with a long-chain structure is formed. The preparation method of the polystyrene hydrophilic fluorescent microsphere has the advantages of simple process steps, simple and accessible raw materials and low cost, and is easy to widely popularize and use. A cTnI content detection card prepared from the polystyrene hydrophilic fluorescent microsphere has higher detection sensitivity and stability, and has higher practicability.
Description
technical field [0001] The application relates to the technical field of medical materials, in particular to a preparation method of polystyrene hydrophilic fluorescent microspheres, the prepared microspheres and applications. Background technique [0002] As a new type of functionalized solid carrier, polystyrene fluorescent microspheres have the characteristics of solid carrier and fluorescent material, and have been widely used in detection devices such as flow cytometry, biosensors, and immunodiagnostic reagents. Polystyrene fluorescent microspheres can emit different wavelengths of fluorescence under the action of external light, so that the target can be analyzed and detected through the fluorescence signal. The surface of polystyrene fluorescent microspheres is modified with various functional groups, which are coupled with target biomolecules (such as proteins, enzymes, nucleic acids, etc.) through covalent bonding of functional groups. [0003] Carboxyl group is a ...
Claims
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Login to View More IPC IPC(8): C08J7/16C08J7/14C08J7/12C08J7/00C08F257/02C08F220/06C09K11/06G01N21/64C08L25/06
CPCC08J7/16C08J7/14C08J7/12C08J7/00C08F257/02C09K11/06G01N21/6402C09K2211/14C08J2325/06C08F220/06
Inventor 周国庆童鎏耿娅妮
Owner 南京基蛋生物医药有限公司