Preparation method for improving yield of beta-cyclodextrin

Abstract

The invention provides a preparation method for improving the yield of beta-cyclodextrin, and belongs to the technical field of cyclodextrin production. Immobilized cyclodextrin glucosyltransferase and immobilized cyclodextrin glucosyltransferase producing bacteria are prepared by using a reversible soluble pH sensitive polymer carrier Eudragit S-100, and the prepared immobilized cyclodextrin glucosyltransferase and immobilized cyclodextrin glucosyltransferase producing bacteria are used for producing beta-cyclodextrin, so that the separation of enzyme and bacteria from products is realized, and the stability and reusability of the enzyme and bacteria are improved; in addition, the cyclodextrin glucosyltransferase producing bacteria are added into a reaction system, glucose generated by enzyme reaction can be utilized, ethanol is generated, the coupling reaction and hydrolysis reaction of the cyclodextrin glucosyltransferase are inhibited, and the cyclization reaction of the cyclodextrin glucosyltransferase is promoted; and meanwhile, the cyclization reaction of the cyclodextrin glucosyltransferase and the growth of the cyclodextrin glucosyltransferase producing bacteria to generate the cyclodextrin glucosyltransferase are synergistic, so that the yield of p-cyclodextrin is improved.

Description

technical field [0001] The invention relates to the technical field of cyclodextrin production, in particular to a preparation method for improving the yield of β-cyclodextrin. Background technique [0002] Cyclodextrin is produced by D-glucopyranose. - Cyclic compounds linked by 1,4-glucoside bonds, among which α-, β- and γ-cyclodextrins composed of 6, 7 and 8 glucose units are the most common. Cyclodextrin can form clathrates with many hydrophobic molecules, thereby changing their physical and chemical properties. Therefore, it is widely used in food, medicine, chemical industry, agriculture and other fields. α, β and γ cyclodextrins are all white crystal powders, among which β-cyclodextrin has the lowest water solubility, so it is easy to crystallize and purify, so β-cyclodextrin is currently produced and widely used in industry. [0003] The industrialized production of cyclodextrin mainly adopts enzymatic process, that is, under the catalysis of cyclodextrin glucosylt...

AI Technical Summary

Problems solved by technology

Although the addition of organic solvents can reduce product inhibition and increase the yield of cyclodextrins, the industrial application of cyclodextrins is limited to a certain extent due to its toxicity and danger.

Moreover, cyclodextrin glucosyltransferase has the functions of catalyzing cyclization, coupling and hydrolysis at the same time. It means that cyclodextrin will be hydrolyzed when the cyclization is not dominant, and the presence of small molecular sugars inhibits the cyclization reaction and promotes the coupling and hydrolysis reactions, thereby affecting the final yield

In addition, the enzymatic process has the disadvantages of one-time use of free enzymes, which cannot be recycled and reused, and the free enzymes are mixed with the product, which increases the burden of separation and purification.

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