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Kit for quantitatively detecting thyroid peroxidase and non-diagnostic purpose detection method

A technology of peroxidase and thyroid, which is applied in the direction of measuring devices, biomaterial analysis, instruments, etc., to achieve the effect of wide detection range

Pending Publication Date: 2020-12-08
ZHENGZHOU IMMUNO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Thyroid peroxidase (TPO), the core biological material that needs to be used in the detection of human thyroid peroxidase antibody, is currently used as the expression system of TPO in insect cells-baculovirus expression system, but how to prepare TPO Quantitatively detect the content of TPO, and then screen and optimize the culture conditions of highly expressed recombinant thyroid peroxidase, but there is no relevant report

Method used

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  • Kit for quantitatively detecting thyroid peroxidase and non-diagnostic purpose detection method
  • Kit for quantitatively detecting thyroid peroxidase and non-diagnostic purpose detection method
  • Kit for quantitatively detecting thyroid peroxidase and non-diagnostic purpose detection method

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Embodiment 1

[0062] Embodiment 1 Kit of the present invention and preparation method thereof

[0063] One, kit of the present invention comprises:

[0064] 1. 0.05M pH9.6 CB buffer formula:

[0065] item name Raw material requirements standard volume per liter purified water Self-made, qualified 1000mL Na 2 CO 3

Analytical pure 1.99g NaHCO 3

Analytical pure 2.53g

[0066] 2. Formula of Casein blocking solution:

[0067]

[0068]

[0069] 3. Formula of sample diluent (pH=7.4):

[0070]

[0071] 4. Formula of enzyme diluent (pH=7.4):

[0072] product name Raw material requirements Theoretical dosage purified water Self-made, qualified 1000mL PBST Qualified 20g bovine serum albumin Qualified 10g P-300 Qualified 1.0mL

[0073] 5. Mouse anti-human TPO antibody;

[0074] 6. Antigen standard (SDS-PAGE quantification, more than 95% purity);

[0075] 7. Horseradish peroxidase-label...

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Abstract

The invention relates to the technical field of biology, in particular to a kit for quantitatively detecting thyroid peroxidase and a non-diagnostic purpose detection method. The kit comprises a microwell plate, a buffer solution, a confining liquid, a sample diluent, an enzyme diluent, a thyroid peroxidase standard substance, a thyroid peroxidase antibody, a biological enzyme labeled thyroid peroxidase antibody and a luminous substrate, the sample diluent comprises a thyroid peroxidase antibody, PBST, newborn calf serum, a preservative and water. According to the invention, a double-antibodysandwich two-step method is established through an ELISA (Enzyme Linked Immuno Sorbent Assay) method, so that the content of human thyroid peroxidase can be conveniently, timely and accurately detected when the human thyroid peroxidase is researched, developed, recombined and naturally extracted, and the detection range is wide.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for quantitatively detecting thyroid peroxidase and a non-diagnostic detection method. Background technique [0002] Thyroid peroxidase (TPO) is the main antigenic component of thyroid microsomes, which is synthesized by thyroid follicular cells. It is a 10% glycated hemoglobin-like protein with a molecular weight of 103kD composed of 933 amino acid residues. The distribution of microvilli on the luminal surface of the follicle is the most abundant. As an important enzyme in the synthesis of thyroid hormones, TPO can catalyze the activation of iodine, the iodination of tyrosine residues on thyroglobulin and the coupling of iodinated tyrosine residues, and finally generate thyroxine (T3, T4), TPO is also the main antigenic component of thyroid microsomes. When thyroid disease occurs and the follicular cell structure is destroyed, TPO leaks from the edge of the thyroid follicula...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/573G01N33/543G01N33/535
CPCG01N33/577G01N33/573G01N33/54306G01N33/535G01N2333/908
Inventor 陈超周雷鸣孙静静王格于婷赵巧辉李桂林付光宇吴学炜杨增利
Owner ZHENGZHOU IMMUNO BIOTECH
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